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3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

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A 3D bioceramic part with inter-porous structure and dimensions of Ø 15 × 6.5 mm produced using laser-aided gelling:(a) isometric view, (b) top view and (c) cross section.
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pone.0143713.g008: A 3D bioceramic part with inter-porous structure and dimensions of Ø 15 × 6.5 mm produced using laser-aided gelling:(a) isometric view, (b) top view and (c) cross section.

Mentions: The optimal material prescription and heat treatment temperature were used to produce an inter-porous bioceramic model for use in bone scaffolds. The inter-porous bioceramic scaffold model, designed using the Solidworks software, has dimensions of Ø 15x6.5 mm, a pore size of 0.8x0.8 mm and a pore distance of 0.8 mm. The forming conditions were a laser scanning velocity of 100 mm/s, a laser power of 3.5 W, a scanning pitch of 0.1 mm, a laser frequency of 10 kHz and a thickness per layer of 0.1 mm, for 65 layers. Fig 8a shows the 3D bio-ceramic model produced by laser-aided gelling. The pores in the scaffolds were connected, as shown in Fig 8b and 8c shows an inner channel in horizontal section, demonstrating that all channels in the scaffold structure were connected, based on the light emitted from the porous parts. The use of LAG to form scaffolds allows allowed the pore shape, size and arrangement to be controlled.


3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

A 3D bioceramic part with inter-porous structure and dimensions of Ø 15 × 6.5 mm produced using laser-aided gelling:(a) isometric view, (b) top view and (c) cross section.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664392&req=5

pone.0143713.g008: A 3D bioceramic part with inter-porous structure and dimensions of Ø 15 × 6.5 mm produced using laser-aided gelling:(a) isometric view, (b) top view and (c) cross section.
Mentions: The optimal material prescription and heat treatment temperature were used to produce an inter-porous bioceramic model for use in bone scaffolds. The inter-porous bioceramic scaffold model, designed using the Solidworks software, has dimensions of Ø 15x6.5 mm, a pore size of 0.8x0.8 mm and a pore distance of 0.8 mm. The forming conditions were a laser scanning velocity of 100 mm/s, a laser power of 3.5 W, a scanning pitch of 0.1 mm, a laser frequency of 10 kHz and a thickness per layer of 0.1 mm, for 65 layers. Fig 8a shows the 3D bio-ceramic model produced by laser-aided gelling. The pores in the scaffolds were connected, as shown in Fig 8b and 8c shows an inner channel in horizontal section, demonstrating that all channels in the scaffold structure were connected, based on the light emitted from the porous parts. The use of LAG to form scaffolds allows allowed the pore shape, size and arrangement to be controlled.

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

Show MeSH
Related in: MedlinePlus