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3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

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The microstructure of CS5 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.
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pone.0143713.g004: The microstructure of CS5 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.

Mentions: When the heat treatment temperature for CS5 is 900°C, the surface separates out CaSiO3 if CaCO3 is added and, as observed in Fig 4a, the surface is evenly covered with rhombic-like crystals with a size of approximately 1 μm. When the temperature is set to 1100°C, the surface begins to grow needle-like crystals characteristic, as shown in Fig 4b. Long noted that needle-like crystal was β-CaSiO3 [27]. When the heat treatment temperature is 1300°C, β-CaSiO3 crystals are also observed on the CS5 surface, and the needle-like crystals are larger than those at 1100°C. The needle-like crystals have a lattice structure and can resist stress rupture, resulting in a significant improvement in compressive strength, as shown in Figs 2a and 4c. When the temperature is 1500°C, the needle-like crystals disappear and oval-like crystals characteristic of α-CaSiO3 begin to precipitate, as shown in Fig 4d. It has been previously demonstrated that oval-like crystal is α-CaSiO3 [28].


3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

The microstructure of CS5 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664392&req=5

pone.0143713.g004: The microstructure of CS5 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.
Mentions: When the heat treatment temperature for CS5 is 900°C, the surface separates out CaSiO3 if CaCO3 is added and, as observed in Fig 4a, the surface is evenly covered with rhombic-like crystals with a size of approximately 1 μm. When the temperature is set to 1100°C, the surface begins to grow needle-like crystals characteristic, as shown in Fig 4b. Long noted that needle-like crystal was β-CaSiO3 [27]. When the heat treatment temperature is 1300°C, β-CaSiO3 crystals are also observed on the CS5 surface, and the needle-like crystals are larger than those at 1100°C. The needle-like crystals have a lattice structure and can resist stress rupture, resulting in a significant improvement in compressive strength, as shown in Figs 2a and 4c. When the temperature is 1500°C, the needle-like crystals disappear and oval-like crystals characteristic of α-CaSiO3 begin to precipitate, as shown in Fig 4d. It has been previously demonstrated that oval-like crystal is α-CaSiO3 [28].

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

Show MeSH
Related in: MedlinePlus