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3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

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The microstructure of CS0 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.
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pone.0143713.g003: The microstructure of CS0 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.

Mentions: To better understand why CS5 presented the best mechanical strength at 1300°C, the surface topographies of specimens CS0, CS5 and CS9 were examined using SEM. When the heat treatment temperature for CS0 is set at 900°C, part of the SiO2 sol gradually recrystallizes and fills the pore space, covering the surface with massive SiO2 crystals, as shown in Fig 3a. At 1100°C, the SiO2 crystals on the surface of CS0 begin to agglomerate and SiO2 sol coats the SiO2 powder as shown in Fig 3b. At 1300°C, most of the SiO2 sol on the surface of CS0 agglomerates into larger pieces, as shown in Fig 3c. At 1500°C, the surface of CS0 appears to be a massive smooth structure and all of the SiO2 powder is coated by SiO2 sol, as shown in Fig 3d. The structure is denser than the structures produced at the other three heat treatment temperatures, resulting in a higher compressive strength. The compressive strength gradually increases along with the bonding strength of the SiO2 sol, as shown in Fig 2a.


3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

The microstructure of CS0 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664392&req=5

pone.0143713.g003: The microstructure of CS0 after heat treatment at various temperatures:(a) 900°C, (b) 1100°C, (c) 1300°C and (d) 1500°C.
Mentions: To better understand why CS5 presented the best mechanical strength at 1300°C, the surface topographies of specimens CS0, CS5 and CS9 were examined using SEM. When the heat treatment temperature for CS0 is set at 900°C, part of the SiO2 sol gradually recrystallizes and fills the pore space, covering the surface with massive SiO2 crystals, as shown in Fig 3a. At 1100°C, the SiO2 crystals on the surface of CS0 begin to agglomerate and SiO2 sol coats the SiO2 powder as shown in Fig 3b. At 1300°C, most of the SiO2 sol on the surface of CS0 agglomerates into larger pieces, as shown in Fig 3c. At 1500°C, the surface of CS0 appears to be a massive smooth structure and all of the SiO2 powder is coated by SiO2 sol, as shown in Fig 3d. The structure is denser than the structures produced at the other three heat treatment temperatures, resulting in a higher compressive strength. The compressive strength gradually increases along with the bonding strength of the SiO2 sol, as shown in Fig 2a.

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

Show MeSH
Related in: MedlinePlus