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3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

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Related in: MedlinePlus

The compressive strength of specimens of CS0, CS5 and CS9 for different heat treatment temperatures:(a) compressive strength, (b) density, (c) volume expansion and (d) porosity.
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pone.0143713.g002: The compressive strength of specimens of CS0, CS5 and CS9 for different heat treatment temperatures:(a) compressive strength, (b) density, (c) volume expansion and (d) porosity.

Mentions: Fig 2a shows the compressive strength of these specimens tested after heat treatment at different temperatures from 900–1500°C. It can be seen that the compressive strength of CS0 is improved with increasing temperature, and it is 36 MPa for the temperature of 1500°C. CS5 and CS9 demonstrated higher compressive strength than CS0 at 900°C, but their compressive strengths did not show any obvious change when the heat treatment temperature was increased from 900°C to 1100°C. At 1100°C, CS5 demonstrated higher compressive strength than CS0, but CS9 showed similar compressive strength to CS0. At 1300°C, the compressive strength of CS5 was obviously improved. The highest compressive strength is 47 MPa, which represents a 30% improvement over CS0 and CS9. The compressive strength of CS9 shows no obvious improvement at 1300°C. When the temperature of the heat treatment was increased to 1500°C, the strengths of both CS5 and CS9 were dramatically decreased. The compressive strength of CS9 was lower than the compressive strength of CS0, whereas the compressive strength of CS5 was only 2 MPa higher than CS0 at 1500°C. The above data show that the mechanical property of the bone scaffold is clearly improved not only by the addition of CaCO3 but also by increasing the sintering temperature.


3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity.

Chang CH, Lin CY, Liu FH, Chen MH, Lin CP, Ho HN, Liao YS - PLoS ONE (2015)

The compressive strength of specimens of CS0, CS5 and CS9 for different heat treatment temperatures:(a) compressive strength, (b) density, (c) volume expansion and (d) porosity.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664392&req=5

pone.0143713.g002: The compressive strength of specimens of CS0, CS5 and CS9 for different heat treatment temperatures:(a) compressive strength, (b) density, (c) volume expansion and (d) porosity.
Mentions: Fig 2a shows the compressive strength of these specimens tested after heat treatment at different temperatures from 900–1500°C. It can be seen that the compressive strength of CS0 is improved with increasing temperature, and it is 36 MPa for the temperature of 1500°C. CS5 and CS9 demonstrated higher compressive strength than CS0 at 900°C, but their compressive strengths did not show any obvious change when the heat treatment temperature was increased from 900°C to 1100°C. At 1100°C, CS5 demonstrated higher compressive strength than CS0, but CS9 showed similar compressive strength to CS0. At 1300°C, the compressive strength of CS5 was obviously improved. The highest compressive strength is 47 MPa, which represents a 30% improvement over CS0 and CS9. The compressive strength of CS9 shows no obvious improvement at 1300°C. When the temperature of the heat treatment was increased to 1500°C, the strengths of both CS5 and CS9 were dramatically decreased. The compressive strength of CS9 was lower than the compressive strength of CS0, whereas the compressive strength of CS5 was only 2 MPa higher than CS0 at 1500°C. The above data show that the mechanical property of the bone scaffold is clearly improved not only by the addition of CaCO3 but also by increasing the sintering temperature.

Bottom Line: Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold.The compressive strength was 47 MPa, and the porosity was 34%.The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

Show MeSH
Related in: MedlinePlus