Limits...
Investigating Tissue Optical Properties and Texture Descriptors of the Retina in Patients with Multiple Sclerosis.

Varga BE, Gao W, Laurik KL, Tátrai E, Simó M, Somfai GM, Cabrera DeBuc D - PLoS ONE (2015)

Bottom Line: Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups.A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons.Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.

ABSTRACT

Purpose: To assess the differences in texture descriptors and optical properties of retinal tissue layers in patients with multiple sclerosis (MS) and to evaluate their usefulness in the detection of neurodegenerative changes using optical coherence tomography (OCT) image segmentation.

Patients and methods: 38 patients with MS were examined using Stratus OCT. The raw macular OCT data were exported and processed using OCTRIMA software. The enrolled eyes were divided into two groups, based on the presence of optic neuritis (ON) in the history (MSON+ group, n = 36 and MSON- group, n = 31). Data of 29 eyes of 24 healthy subjects (H) were used as controls. A total of seven intraretinal layers were segmented and thickness as well as optical parameters such as contrast, fractal dimension, layer index and total reflectance were measured. Mixed-model ANOVA analysis was used for statistical comparisons.

Results: Significant thinning of the retinal nerve fiber layer (RNFL), ganglion cell/inner plexiform layer complex (GCL+IPL) and ganglion cell complex (GCC, RNFL+GCL+IPL) was observed between study groups in all comparisons. Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups. Higher fractal dimension values were observed in GCL+IPL and INL layers when comparing H vs. MSON+ groups. A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons. A significant difference was observed in total reflectance in the RNFL, GCL+IPL and GCC layers between the three examination groups.

Conclusion: Texture and optical properties of the retinal tissue undergo pronounced changes in MS even without optic neuritis. Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration.

Show MeSH

Related in: MedlinePlus

Macular image segmentation results using OCTRIMA.(A) The image of a healthy macula scanned by Stratus OCT with the division of the macular regions used for the analysis (foveolar region (a) with a diameter of 0.375 mm, foveal region (b) with a diameter of 1.85 mm; parafoveal region (c) with a diameter of 2.85 mm and perifoveal region (d) with a diameter of 5.85 mm) (B) The same OCT scan processed with OCTRIMA. Abbreviations: Ch, choroid; GCL+IPL, ganglion cell layer and inner plexiform layer complex; INL, inner nuclear layer; ONL+IS, combined outer nuclear layer and inner segment of photoreceptors; OS, outer segment of photoreceptors; OPL, outer plexiform layer; RNFL, retinal nerve fiber layer; RPE, retinal pigment epithelial layer; V, vitreous.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664388&req=5

pone.0143711.g001: Macular image segmentation results using OCTRIMA.(A) The image of a healthy macula scanned by Stratus OCT with the division of the macular regions used for the analysis (foveolar region (a) with a diameter of 0.375 mm, foveal region (b) with a diameter of 1.85 mm; parafoveal region (c) with a diameter of 2.85 mm and perifoveal region (d) with a diameter of 5.85 mm) (B) The same OCT scan processed with OCTRIMA. Abbreviations: Ch, choroid; GCL+IPL, ganglion cell layer and inner plexiform layer complex; INL, inner nuclear layer; ONL+IS, combined outer nuclear layer and inner segment of photoreceptors; OS, outer segment of photoreceptors; OPL, outer plexiform layer; RNFL, retinal nerve fiber layer; RPE, retinal pigment epithelial layer; V, vitreous.

Mentions: The radial scans were exported and processed by a custom-built software (OCTRIMA) developed by Cabrera et al. [6]. This OCT image segmentation program is capable of segmenting 7 cellular layers of the retina based on their optical densities (see Fig 1): the RNFL, the ganglion cell and inner plexiform layer complex (GCL+IPL), the INL, the outer plexiform layer (OPL), the outer nuclear layer and inner photoreceptor segment (ONL+IS), outer photoreceptor segment (OS) and retinal pigment epithelium (RPE). [6] Because of the special arrangement of the retinal structure below the foveal pit where the inner retinal layers are displaced concentrally, the segmentation of the foveolar region was limited to the three outer retinal layers (ONL+IS, OS, RPE).


Investigating Tissue Optical Properties and Texture Descriptors of the Retina in Patients with Multiple Sclerosis.

Varga BE, Gao W, Laurik KL, Tátrai E, Simó M, Somfai GM, Cabrera DeBuc D - PLoS ONE (2015)

Macular image segmentation results using OCTRIMA.(A) The image of a healthy macula scanned by Stratus OCT with the division of the macular regions used for the analysis (foveolar region (a) with a diameter of 0.375 mm, foveal region (b) with a diameter of 1.85 mm; parafoveal region (c) with a diameter of 2.85 mm and perifoveal region (d) with a diameter of 5.85 mm) (B) The same OCT scan processed with OCTRIMA. Abbreviations: Ch, choroid; GCL+IPL, ganglion cell layer and inner plexiform layer complex; INL, inner nuclear layer; ONL+IS, combined outer nuclear layer and inner segment of photoreceptors; OS, outer segment of photoreceptors; OPL, outer plexiform layer; RNFL, retinal nerve fiber layer; RPE, retinal pigment epithelial layer; V, vitreous.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664388&req=5

pone.0143711.g001: Macular image segmentation results using OCTRIMA.(A) The image of a healthy macula scanned by Stratus OCT with the division of the macular regions used for the analysis (foveolar region (a) with a diameter of 0.375 mm, foveal region (b) with a diameter of 1.85 mm; parafoveal region (c) with a diameter of 2.85 mm and perifoveal region (d) with a diameter of 5.85 mm) (B) The same OCT scan processed with OCTRIMA. Abbreviations: Ch, choroid; GCL+IPL, ganglion cell layer and inner plexiform layer complex; INL, inner nuclear layer; ONL+IS, combined outer nuclear layer and inner segment of photoreceptors; OS, outer segment of photoreceptors; OPL, outer plexiform layer; RNFL, retinal nerve fiber layer; RPE, retinal pigment epithelial layer; V, vitreous.
Mentions: The radial scans were exported and processed by a custom-built software (OCTRIMA) developed by Cabrera et al. [6]. This OCT image segmentation program is capable of segmenting 7 cellular layers of the retina based on their optical densities (see Fig 1): the RNFL, the ganglion cell and inner plexiform layer complex (GCL+IPL), the INL, the outer plexiform layer (OPL), the outer nuclear layer and inner photoreceptor segment (ONL+IS), outer photoreceptor segment (OS) and retinal pigment epithelium (RPE). [6] Because of the special arrangement of the retinal structure below the foveal pit where the inner retinal layers are displaced concentrally, the segmentation of the foveolar region was limited to the three outer retinal layers (ONL+IS, OS, RPE).

Bottom Line: Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups.A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons.Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.

ABSTRACT

Purpose: To assess the differences in texture descriptors and optical properties of retinal tissue layers in patients with multiple sclerosis (MS) and to evaluate their usefulness in the detection of neurodegenerative changes using optical coherence tomography (OCT) image segmentation.

Patients and methods: 38 patients with MS were examined using Stratus OCT. The raw macular OCT data were exported and processed using OCTRIMA software. The enrolled eyes were divided into two groups, based on the presence of optic neuritis (ON) in the history (MSON+ group, n = 36 and MSON- group, n = 31). Data of 29 eyes of 24 healthy subjects (H) were used as controls. A total of seven intraretinal layers were segmented and thickness as well as optical parameters such as contrast, fractal dimension, layer index and total reflectance were measured. Mixed-model ANOVA analysis was used for statistical comparisons.

Results: Significant thinning of the retinal nerve fiber layer (RNFL), ganglion cell/inner plexiform layer complex (GCL+IPL) and ganglion cell complex (GCC, RNFL+GCL+IPL) was observed between study groups in all comparisons. Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups. Higher fractal dimension values were observed in GCL+IPL and INL layers when comparing H vs. MSON+ groups. A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons. A significant difference was observed in total reflectance in the RNFL, GCL+IPL and GCC layers between the three examination groups.

Conclusion: Texture and optical properties of the retinal tissue undergo pronounced changes in MS even without optic neuritis. Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration.

Show MeSH
Related in: MedlinePlus