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Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

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Naringin suppressed NF-κ B signaling pathway activation.(A) The protein levels of p-I κ B α, I κ B α and NF-κ B in kidney tissues and HBZY-1 cells were determined by western blot. Results represent three independent experiments. (B) The distribution change of NF-κ B in HBZY-1 cells was observed by immunofluorescence assay. Results represent three independent experiments. (C) The DNA binding activities of NF-κ B in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5.a-c Means with different superscripts are significantly different (P< 0.05).
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pone.0143868.g007: Naringin suppressed NF-κ B signaling pathway activation.(A) The protein levels of p-I κ B α, I κ B α and NF-κ B in kidney tissues and HBZY-1 cells were determined by western blot. Results represent three independent experiments. (B) The distribution change of NF-κ B in HBZY-1 cells was observed by immunofluorescence assay. Results represent three independent experiments. (C) The DNA binding activities of NF-κ B in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5.a-c Means with different superscripts are significantly different (P< 0.05).

Mentions: Western blot assay revealed that the expression of NF-κ B in the cytoplasm was decreased and that in the nuclei was increased in diabetic rats or high glucose treated HBZY-1 cells, which could be significantly reversed by naringin treatment (Fig 7A). The level of I κ B α protein was decreased and that of p-I κ B α was increased significantly induced by STZ or high glucose compared with control group. This change was significantly inhibited by naringin treatment. Subsequently, the distribution of NF-κ B was determined by immunofluorescence staining. As shown in Fig 7B, obvious distribution change of NF-κ B from cytoplasm to nucleus was induced by high glucose, whereas treatment with naringin significantly inhibited the distribution change of NF-κ B. To further confirm the effect of naringin on NF-κ B activation, the DNA binding activity of NF-κ B was detected by EMSA assay. As shown in Fig 7C, the DNA-binding activity of NF-κ B was increased (P<0.05) in diabetic rats compared with control group, which could be inhibited by treatment with naringin.


Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Naringin suppressed NF-κ B signaling pathway activation.(A) The protein levels of p-I κ B α, I κ B α and NF-κ B in kidney tissues and HBZY-1 cells were determined by western blot. Results represent three independent experiments. (B) The distribution change of NF-κ B in HBZY-1 cells was observed by immunofluorescence assay. Results represent three independent experiments. (C) The DNA binding activities of NF-κ B in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5.a-c Means with different superscripts are significantly different (P< 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664292&req=5

pone.0143868.g007: Naringin suppressed NF-κ B signaling pathway activation.(A) The protein levels of p-I κ B α, I κ B α and NF-κ B in kidney tissues and HBZY-1 cells were determined by western blot. Results represent three independent experiments. (B) The distribution change of NF-κ B in HBZY-1 cells was observed by immunofluorescence assay. Results represent three independent experiments. (C) The DNA binding activities of NF-κ B in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5.a-c Means with different superscripts are significantly different (P< 0.05).
Mentions: Western blot assay revealed that the expression of NF-κ B in the cytoplasm was decreased and that in the nuclei was increased in diabetic rats or high glucose treated HBZY-1 cells, which could be significantly reversed by naringin treatment (Fig 7A). The level of I κ B α protein was decreased and that of p-I κ B α was increased significantly induced by STZ or high glucose compared with control group. This change was significantly inhibited by naringin treatment. Subsequently, the distribution of NF-κ B was determined by immunofluorescence staining. As shown in Fig 7B, obvious distribution change of NF-κ B from cytoplasm to nucleus was induced by high glucose, whereas treatment with naringin significantly inhibited the distribution change of NF-κ B. To further confirm the effect of naringin on NF-κ B activation, the DNA binding activity of NF-κ B was detected by EMSA assay. As shown in Fig 7C, the DNA-binding activity of NF-κ B was increased (P<0.05) in diabetic rats compared with control group, which could be inhibited by treatment with naringin.

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

Show MeSH
Related in: MedlinePlus