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Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

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Naringin inhibited inflammatory reaction in kidney tissues and HBZY-1 cells.The TNF-α(A), MCP-1(B), ICAM-1(C) and VCAM-1(D) levels in kidney tissues were determined by ELISA assay. Data were expressed as means ± SD, n = 6. The TNF-α(E), MCP-1(F), ICAM-1(G) and VCAM-1(H) levels in HBZY-1 cells were determined by ELISA assay. Data were expressed as means ± SD, n = 3.a-d Means with different superscripts are significantly different (P< 0.05).
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pone.0143868.g006: Naringin inhibited inflammatory reaction in kidney tissues and HBZY-1 cells.The TNF-α(A), MCP-1(B), ICAM-1(C) and VCAM-1(D) levels in kidney tissues were determined by ELISA assay. Data were expressed as means ± SD, n = 6. The TNF-α(E), MCP-1(F), ICAM-1(G) and VCAM-1(H) levels in HBZY-1 cells were determined by ELISA assay. Data were expressed as means ± SD, n = 3.a-d Means with different superscripts are significantly different (P< 0.05).

Mentions: To assess the effect of naringin on inflammatory reaction, some representative inflammatory markers and related molecules were measured by ELISA. As shown in Fig 6, the levels of TNF-α, MCP-1, ICAM-1 and VCAM-1 were obviously increased (P<0.05) both in diabetic rats and in high glucose treated HBZY-1 cells. However, the levels of these markers were decreased significantly by naringin treatment.


Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Naringin inhibited inflammatory reaction in kidney tissues and HBZY-1 cells.The TNF-α(A), MCP-1(B), ICAM-1(C) and VCAM-1(D) levels in kidney tissues were determined by ELISA assay. Data were expressed as means ± SD, n = 6. The TNF-α(E), MCP-1(F), ICAM-1(G) and VCAM-1(H) levels in HBZY-1 cells were determined by ELISA assay. Data were expressed as means ± SD, n = 3.a-d Means with different superscripts are significantly different (P< 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664292&req=5

pone.0143868.g006: Naringin inhibited inflammatory reaction in kidney tissues and HBZY-1 cells.The TNF-α(A), MCP-1(B), ICAM-1(C) and VCAM-1(D) levels in kidney tissues were determined by ELISA assay. Data were expressed as means ± SD, n = 6. The TNF-α(E), MCP-1(F), ICAM-1(G) and VCAM-1(H) levels in HBZY-1 cells were determined by ELISA assay. Data were expressed as means ± SD, n = 3.a-d Means with different superscripts are significantly different (P< 0.05).
Mentions: To assess the effect of naringin on inflammatory reaction, some representative inflammatory markers and related molecules were measured by ELISA. As shown in Fig 6, the levels of TNF-α, MCP-1, ICAM-1 and VCAM-1 were obviously increased (P<0.05) both in diabetic rats and in high glucose treated HBZY-1 cells. However, the levels of these markers were decreased significantly by naringin treatment.

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

Show MeSH
Related in: MedlinePlus