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Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

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Naringin restrained oxidative stress injury by activating Nrf2 antioxidant pathway.The concentration of MDA (A), the activity of SOD(B) and GSH-Px(C) in kidney tissues were detected. Data were expressed as means ± SD, n = 6. (D) The ROS production in HBZY-1 cells was evaluated by flow cytometry and ROS generation rates were shown. Data were expressed as means ± SD, n = 3. (E) The protein levels of Nrf2 and HO-1 in kidney tissues or HBZY-1 cells were detected by western blot. Results represent three independent experiments. (F) The DNA binding activities of Nrf2 in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5. (G) The HO-1 activities in kidney tissues were determined. Data were expressed as means ± SD, n = 6.a-d Means with different superscripts are significantly different (P< 0.05).
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pone.0143868.g005: Naringin restrained oxidative stress injury by activating Nrf2 antioxidant pathway.The concentration of MDA (A), the activity of SOD(B) and GSH-Px(C) in kidney tissues were detected. Data were expressed as means ± SD, n = 6. (D) The ROS production in HBZY-1 cells was evaluated by flow cytometry and ROS generation rates were shown. Data were expressed as means ± SD, n = 3. (E) The protein levels of Nrf2 and HO-1 in kidney tissues or HBZY-1 cells were detected by western blot. Results represent three independent experiments. (F) The DNA binding activities of Nrf2 in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5. (G) The HO-1 activities in kidney tissues were determined. Data were expressed as means ± SD, n = 6.a-d Means with different superscripts are significantly different (P< 0.05).

Mentions: To evaluate the oxidative stress injury, the levels or activities of MDA, SOD, GSH-Px, and ROS were determined. As shown in Fig 5A–5D, the levels of ROS and MDA were significantly elevated and activities of SOD and GSH-Px were reduced (P<0.05) in kidney tissues of diabetic rats or high glucose-induced HBZY-1 cells. However, treatment with naringin could result in decreased levels of ROS and MDA and increased activities of SOD and GSH-Px. Moreover, the expression and activity of Nrf2, the key regulator of the antioxidative signaling pathway, and its downstream target HO-1 was observed. As assayed by western blot and shown in Fig 5E, Nrf2 expression in the nuclei was increased induced by STZ in vivo and high glucose in vitro, which could be promoted by treatment with naringin. Moreover, the expression and activity of HO-1 was accordingly increased (P<0.05) by treatment with naringin (Fig 5E and 5G). As shown in Fig 5F, the DNA binding activity of Nrf2 was significantly increased by naringin treatment (P<0.05).


Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Naringin restrained oxidative stress injury by activating Nrf2 antioxidant pathway.The concentration of MDA (A), the activity of SOD(B) and GSH-Px(C) in kidney tissues were detected. Data were expressed as means ± SD, n = 6. (D) The ROS production in HBZY-1 cells was evaluated by flow cytometry and ROS generation rates were shown. Data were expressed as means ± SD, n = 3. (E) The protein levels of Nrf2 and HO-1 in kidney tissues or HBZY-1 cells were detected by western blot. Results represent three independent experiments. (F) The DNA binding activities of Nrf2 in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5. (G) The HO-1 activities in kidney tissues were determined. Data were expressed as means ± SD, n = 6.a-d Means with different superscripts are significantly different (P< 0.05).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4664292&req=5

pone.0143868.g005: Naringin restrained oxidative stress injury by activating Nrf2 antioxidant pathway.The concentration of MDA (A), the activity of SOD(B) and GSH-Px(C) in kidney tissues were detected. Data were expressed as means ± SD, n = 6. (D) The ROS production in HBZY-1 cells was evaluated by flow cytometry and ROS generation rates were shown. Data were expressed as means ± SD, n = 3. (E) The protein levels of Nrf2 and HO-1 in kidney tissues or HBZY-1 cells were detected by western blot. Results represent three independent experiments. (F) The DNA binding activities of Nrf2 in kidney tissues were assessed by EMSA assay. Data were expressed as means ± SD, n = 5. (G) The HO-1 activities in kidney tissues were determined. Data were expressed as means ± SD, n = 6.a-d Means with different superscripts are significantly different (P< 0.05).
Mentions: To evaluate the oxidative stress injury, the levels or activities of MDA, SOD, GSH-Px, and ROS were determined. As shown in Fig 5A–5D, the levels of ROS and MDA were significantly elevated and activities of SOD and GSH-Px were reduced (P<0.05) in kidney tissues of diabetic rats or high glucose-induced HBZY-1 cells. However, treatment with naringin could result in decreased levels of ROS and MDA and increased activities of SOD and GSH-Px. Moreover, the expression and activity of Nrf2, the key regulator of the antioxidative signaling pathway, and its downstream target HO-1 was observed. As assayed by western blot and shown in Fig 5E, Nrf2 expression in the nuclei was increased induced by STZ in vivo and high glucose in vitro, which could be promoted by treatment with naringin. Moreover, the expression and activity of HO-1 was accordingly increased (P<0.05) by treatment with naringin (Fig 5E and 5G). As shown in Fig 5F, the DNA binding activity of Nrf2 was significantly increased by naringin treatment (P<0.05).

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

Show MeSH
Related in: MedlinePlus