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Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

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Naringin inhibited collagen production and renal interstitial fibrosis.(A) The collagen deposition and renal interstitial fibrosis was observed by Masson’s staining(200×). The percentages of fibrosis area were shown. (B) The expression of collagen I in kidney tissues was determined by immunohistochemical staining(400×). (C) The expressions of collagen I, MMP-2, TIMP-1 and TGF-β1 in kidney tissues were detected by western blot. Results represent three independent experiments. Data were expressed as means ± SD, n = 6.a-c Means with different superscripts are significantly different (P< 0.05).
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pone.0143868.g003: Naringin inhibited collagen production and renal interstitial fibrosis.(A) The collagen deposition and renal interstitial fibrosis was observed by Masson’s staining(200×). The percentages of fibrosis area were shown. (B) The expression of collagen I in kidney tissues was determined by immunohistochemical staining(400×). (C) The expressions of collagen I, MMP-2, TIMP-1 and TGF-β1 in kidney tissues were detected by western blot. Results represent three independent experiments. Data were expressed as means ± SD, n = 6.a-c Means with different superscripts are significantly different (P< 0.05).

Mentions: The collagen production and renal interstitial fibrosis was observed by Masson’s staining. As shown in Fig 3A, administration of naringin effectively alleviated (P<0.05) the collagen deposition and renal interstitial fibrosis in diabetic rats. In addition, the results of western blot and immunohistochemical staining assay suggested that the protein level of collagen type I was up-regulated in diabetic rats compared with control group. However, treatment with naringin could reduce the protein level of collagen type I (Fig 3B and 3C). Moreover, the collagen production and degradation related proteins were assessed by western blot. As shown in Fig 3C, the protein expressions of TIMP-1 and TGF-β1 were increased and that of MMP-2 was decreased in diabetic rats compared with control group, whereas treatment with naringin could inhibit the changes of these proteins.


Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Naringin inhibited collagen production and renal interstitial fibrosis.(A) The collagen deposition and renal interstitial fibrosis was observed by Masson’s staining(200×). The percentages of fibrosis area were shown. (B) The expression of collagen I in kidney tissues was determined by immunohistochemical staining(400×). (C) The expressions of collagen I, MMP-2, TIMP-1 and TGF-β1 in kidney tissues were detected by western blot. Results represent three independent experiments. Data were expressed as means ± SD, n = 6.a-c Means with different superscripts are significantly different (P< 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664292&req=5

pone.0143868.g003: Naringin inhibited collagen production and renal interstitial fibrosis.(A) The collagen deposition and renal interstitial fibrosis was observed by Masson’s staining(200×). The percentages of fibrosis area were shown. (B) The expression of collagen I in kidney tissues was determined by immunohistochemical staining(400×). (C) The expressions of collagen I, MMP-2, TIMP-1 and TGF-β1 in kidney tissues were detected by western blot. Results represent three independent experiments. Data were expressed as means ± SD, n = 6.a-c Means with different superscripts are significantly different (P< 0.05).
Mentions: The collagen production and renal interstitial fibrosis was observed by Masson’s staining. As shown in Fig 3A, administration of naringin effectively alleviated (P<0.05) the collagen deposition and renal interstitial fibrosis in diabetic rats. In addition, the results of western blot and immunohistochemical staining assay suggested that the protein level of collagen type I was up-regulated in diabetic rats compared with control group. However, treatment with naringin could reduce the protein level of collagen type I (Fig 3B and 3C). Moreover, the collagen production and degradation related proteins were assessed by western blot. As shown in Fig 3C, the protein expressions of TIMP-1 and TGF-β1 were increased and that of MMP-2 was decreased in diabetic rats compared with control group, whereas treatment with naringin could inhibit the changes of these proteins.

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

Show MeSH
Related in: MedlinePlus