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Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

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The chemical structure of naringin.The molecular formula of naringin is C27H32O14 and the molecular weight is 580.53.
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pone.0143868.g001: The chemical structure of naringin.The molecular formula of naringin is C27H32O14 and the molecular weight is 580.53.

Mentions: Naringin with purity of ≥ 95% was purchased from Sigma Chemical Co. (St Louis, USA) and dissolved in sterile normal saline as a stock concentration of 20 mg/ml and stored at −20°C in the dark. The chemical structural of naringin is showed in Fig 1. STZ was purchased from Solarbio Science and technology Co., Ltd (Beijing, China). STZ was dissolved in 0.1 M citrate buffer with a concentration of 32.5 mg/ml. The antibodies used in our study were as follows: Collagen I (Boster, China), MMP2 (Boster, China), TIMP-1 (Boster, China), TGF-β1 (Santa Cruz, USA), I κ B α (Bioss, China), p-I κ B α (Bioss, China), NF-κ B (Boster, China), Nrf2 (Boster, China), HO-1 (Santa Cruz, USA), β-actin (Santa Cruz, USA), Histone H3 (Bioss, China).


Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction.

Chen F, Zhang N, Ma X, Huang T, Shao Y, Wu C, Wang Q - PLoS ONE (2015)

The chemical structure of naringin.The molecular formula of naringin is C27H32O14 and the molecular weight is 580.53.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664292&req=5

pone.0143868.g001: The chemical structure of naringin.The molecular formula of naringin is C27H32O14 and the molecular weight is 580.53.
Mentions: Naringin with purity of ≥ 95% was purchased from Sigma Chemical Co. (St Louis, USA) and dissolved in sterile normal saline as a stock concentration of 20 mg/ml and stored at −20°C in the dark. The chemical structural of naringin is showed in Fig 1. STZ was purchased from Solarbio Science and technology Co., Ltd (Beijing, China). STZ was dissolved in 0.1 M citrate buffer with a concentration of 32.5 mg/ml. The antibodies used in our study were as follows: Collagen I (Boster, China), MMP2 (Boster, China), TIMP-1 (Boster, China), TGF-β1 (Santa Cruz, USA), I κ B α (Bioss, China), p-I κ B α (Bioss, China), NF-κ B (Boster, China), Nrf2 (Boster, China), HO-1 (Santa Cruz, USA), β-actin (Santa Cruz, USA), Histone H3 (Bioss, China).

Bottom Line: According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis.Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway.Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

View Article: PubMed Central - PubMed

Affiliation: Department of Geratology for Cadres, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China.

ABSTRACT
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.

Show MeSH
Related in: MedlinePlus