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Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

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Relationship between the area under the reflectance curve and total pigment concentration (symbiont + other contributors).The x-axis represents the integration of the reflectance curve from 400–700 nm. The lower right graph shows the pooled data from all seven species.
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pone.0143709.g007: Relationship between the area under the reflectance curve and total pigment concentration (symbiont + other contributors).The x-axis represents the integration of the reflectance curve from 400–700 nm. The lower right graph shows the pooled data from all seven species.

Mentions: Our previous work with Acropora cervicornis and Porites porites showed the potential use of reflectance to estimate pigment. Here, we applied a similar technique, but using Rrs instead, to a larger number of species. An integration of the area under the Rrs curve was used to evaluate its use within the visible range (400–700 nm) as a proxy for estimating total pigment concentration in reef corals. Fig 6 shows that based on the total symbiont pigments composition and depending on the species, we found 79.5–98.5% predictability with an overall prediction effectiveness of 62% when all the species data is pooled. The prediction effectiveness diminishes when the pigments from additional sources are added (62–95% with an overall of 52%) (Fig 7). While we were limited in the number of colonies that we could sample, a higher sample size (hence, potentially more variability in symbiont and pigment concentration), either from the same sites or from additional sites, may produce different results. Nevertheless, our data shows a persistent pattern exemplified by the high Rrs area and pigment concentration correlations in all species. This can be used as a baseline for suture more comprehensive studies.


Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Relationship between the area under the reflectance curve and total pigment concentration (symbiont + other contributors).The x-axis represents the integration of the reflectance curve from 400–700 nm. The lower right graph shows the pooled data from all seven species.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664284&req=5

pone.0143709.g007: Relationship between the area under the reflectance curve and total pigment concentration (symbiont + other contributors).The x-axis represents the integration of the reflectance curve from 400–700 nm. The lower right graph shows the pooled data from all seven species.
Mentions: Our previous work with Acropora cervicornis and Porites porites showed the potential use of reflectance to estimate pigment. Here, we applied a similar technique, but using Rrs instead, to a larger number of species. An integration of the area under the Rrs curve was used to evaluate its use within the visible range (400–700 nm) as a proxy for estimating total pigment concentration in reef corals. Fig 6 shows that based on the total symbiont pigments composition and depending on the species, we found 79.5–98.5% predictability with an overall prediction effectiveness of 62% when all the species data is pooled. The prediction effectiveness diminishes when the pigments from additional sources are added (62–95% with an overall of 52%) (Fig 7). While we were limited in the number of colonies that we could sample, a higher sample size (hence, potentially more variability in symbiont and pigment concentration), either from the same sites or from additional sites, may produce different results. Nevertheless, our data shows a persistent pattern exemplified by the high Rrs area and pigment concentration correlations in all species. This can be used as a baseline for suture more comprehensive studies.

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

Show MeSH
Related in: MedlinePlus