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Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

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Concentration of symbiont cells per coral tissue area per species.Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.
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pone.0143709.g003: Concentration of symbiont cells per coral tissue area per species.Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.

Mentions: The average concentration of symbiont cells within the coral tissues ranged from 985,705 to 5,001,700 cells per cm-2 of coral tissue and also differed significantly between species (Fig 3; One-Way ANOVA, F = 7.40, p<0.0001) with P. astreoides containing the highest average concentration of symbionts and C. natans the lowest. The overall high variability in symbiont concentration found within most studied species is reflective of the small sample size. Nonetheless, keeping in mind that all samples were collected at the same depth (1m), the range in symbiont concentration among species may reflect the influence of different skeletal arrangements and the consequent differences in light regime reaching the endosymbiotic dinoflagellates within the coral tissue [43]. This in turn affects the concentration and distribution of symbiont cells within the coral tissues and their respective photosysnthetic pigment array. For instance, a coral such as C. natans has a particularly thin meandriod skeletal structure with large polyps while P. astreoides has a denser smoother skeleton with small polyps. This is reflected in their differences in symbiont cocentration with the latter containing 5X that of the former.


Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Concentration of symbiont cells per coral tissue area per species.Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664284&req=5

pone.0143709.g003: Concentration of symbiont cells per coral tissue area per species.Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.
Mentions: The average concentration of symbiont cells within the coral tissues ranged from 985,705 to 5,001,700 cells per cm-2 of coral tissue and also differed significantly between species (Fig 3; One-Way ANOVA, F = 7.40, p<0.0001) with P. astreoides containing the highest average concentration of symbionts and C. natans the lowest. The overall high variability in symbiont concentration found within most studied species is reflective of the small sample size. Nonetheless, keeping in mind that all samples were collected at the same depth (1m), the range in symbiont concentration among species may reflect the influence of different skeletal arrangements and the consequent differences in light regime reaching the endosymbiotic dinoflagellates within the coral tissue [43]. This in turn affects the concentration and distribution of symbiont cells within the coral tissues and their respective photosysnthetic pigment array. For instance, a coral such as C. natans has a particularly thin meandriod skeletal structure with large polyps while P. astreoides has a denser smoother skeleton with small polyps. This is reflected in their differences in symbiont cocentration with the latter containing 5X that of the former.

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

Show MeSH
Related in: MedlinePlus