Limits...
Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

Show MeSH

Related in: MedlinePlus

Pigment contents among the seven coral species studied.Variability in concentration of pigment groups among species. Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664284&req=5

pone.0143709.g002: Pigment contents among the seven coral species studied.Variability in concentration of pigment groups among species. Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.

Mentions: When considering the contribution of the endosymbiotic dinoflagellates to the coral pigment complexes, chlorophylls were the dominant pigment group (both in percentage and concentration) in all the corals with the exception of Siderastrea siderea, where carotenes dominated (particularly peridinin) (Fig 2). There were significant differences between species in terms of chlorophylls concentration (One-Way ANOVA, F = 5.04, p = 0.001) and percentage (Kruskal-Wallis, H = 27.24, p<0.0001), carotenes concentration (One-Way ANOVA, F = 10.43, p<0.0001) and percentage (Kruskal-Wallis, H = 30.49, p<0.0001), and xanthophylls concentration (One-Way ANOVA, F = 6.22, p<0.0001) and percentage (Kruskal-Wallis, H = 13.53, p = 0.035). This is consistent with past findings where the variability among pigment percentages was smaller than that of their respective concentrations within colonies of the same species living at similar depths [12]. Additionally, our results coincide with those of Myers et al [10] who found that “healthy” colonies of A. cervicornis had the lowest relative total pigment, chl a and peridinin density among the coral species studied which included M. (Orbicella) annularis and P. astreoides.


Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Pigment contents among the seven coral species studied.Variability in concentration of pigment groups among species. Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664284&req=5

pone.0143709.g002: Pigment contents among the seven coral species studied.Variability in concentration of pigment groups among species. Coral species abbreviations: A cerv—Acropora cervicornis; C nat—Colpophyllia natans; O ann—Orbicella annularis; P astr—Porites astreoides; P furc—Porites furcata; P stri—Pseudodiploria strigosa; S side—Siderastrea siderea. Error bars indicate ±1SD.
Mentions: When considering the contribution of the endosymbiotic dinoflagellates to the coral pigment complexes, chlorophylls were the dominant pigment group (both in percentage and concentration) in all the corals with the exception of Siderastrea siderea, where carotenes dominated (particularly peridinin) (Fig 2). There were significant differences between species in terms of chlorophylls concentration (One-Way ANOVA, F = 5.04, p = 0.001) and percentage (Kruskal-Wallis, H = 27.24, p<0.0001), carotenes concentration (One-Way ANOVA, F = 10.43, p<0.0001) and percentage (Kruskal-Wallis, H = 30.49, p<0.0001), and xanthophylls concentration (One-Way ANOVA, F = 6.22, p<0.0001) and percentage (Kruskal-Wallis, H = 13.53, p = 0.035). This is consistent with past findings where the variability among pigment percentages was smaller than that of their respective concentrations within colonies of the same species living at similar depths [12]. Additionally, our results coincide with those of Myers et al [10] who found that “healthy” colonies of A. cervicornis had the lowest relative total pigment, chl a and peridinin density among the coral species studied which included M. (Orbicella) annularis and P. astreoides.

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

Show MeSH
Related in: MedlinePlus