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Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

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Coral species studied.A) Acropora cervicornis; B) Colpophyllia natans; C) Orbicella annularis; D) Porites astreoides; E) Porites furcata; F) Pseudodiploria strigosa; G) Siderastrea siderea.
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pone.0143709.g001: Coral species studied.A) Acropora cervicornis; B) Colpophyllia natans; C) Orbicella annularis; D) Porites astreoides; E) Porites furcata; F) Pseudodiploria strigosa; G) Siderastrea siderea.

Mentions: Samples from seven Caribbean shallow-water coral species (Fig 1) (Acropora cervicornis, Colpophyllia natans, Pseudodiploria strigosa, Orbicella annularis (these two species formely known as Diploria strigosa and Montastraea annularis [19], respectively), Porites astreoides, Porites furcata and Siderastrea siderea) were collected. In the case of the branching corals A. cervicornis and P. furcata, one branch was collected from each of seven “visibly healthy” colonies per species at 1 m depth from the back-reef area of San Cristóbal Reef. Colonies sampled were spaced at least 10 m apart to avoid, as much as possible, pseudo-replication or sampling of genetically similar ramets. Here, we define a “visibly healthy” colony as one not showing any signs of disease, discoloration or bleaching [12] compared to the rest of the colony or its neighbors. In the case of massive corals, a 4 cm (diameter) sample was collected with a pneumatic drill attached to a SCUBA tank. Depending on the availability, 4–7 samples were collected from each of the five massive coral species studied. The collection permit from the Puerto Rico Department of Natural and Environmental Resources limited our sampling to ≤ 7 samples per species. The scar left by the drilling was covered with hydraulic cement to avoid colonization of turf algae or any other opportunistic organism. All the samples were transported in aerated seawater in sterilized Whirl-Pak plastic bags and transported to an outdoor aquarium located at the University of Puerto Rico’s Department of Marine Sciences Magueyes Island Field Station (UPR) for immediate processing. The whole transportation process took approximately 30–40 minutes.


Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

Torres-Pérez JL, Guild LS, Armstrong RA, Corredor J, Zuluaga-Montero A, Polanco R - PLoS ONE (2015)

Coral species studied.A) Acropora cervicornis; B) Colpophyllia natans; C) Orbicella annularis; D) Porites astreoides; E) Porites furcata; F) Pseudodiploria strigosa; G) Siderastrea siderea.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664284&req=5

pone.0143709.g001: Coral species studied.A) Acropora cervicornis; B) Colpophyllia natans; C) Orbicella annularis; D) Porites astreoides; E) Porites furcata; F) Pseudodiploria strigosa; G) Siderastrea siderea.
Mentions: Samples from seven Caribbean shallow-water coral species (Fig 1) (Acropora cervicornis, Colpophyllia natans, Pseudodiploria strigosa, Orbicella annularis (these two species formely known as Diploria strigosa and Montastraea annularis [19], respectively), Porites astreoides, Porites furcata and Siderastrea siderea) were collected. In the case of the branching corals A. cervicornis and P. furcata, one branch was collected from each of seven “visibly healthy” colonies per species at 1 m depth from the back-reef area of San Cristóbal Reef. Colonies sampled were spaced at least 10 m apart to avoid, as much as possible, pseudo-replication or sampling of genetically similar ramets. Here, we define a “visibly healthy” colony as one not showing any signs of disease, discoloration or bleaching [12] compared to the rest of the colony or its neighbors. In the case of massive corals, a 4 cm (diameter) sample was collected with a pneumatic drill attached to a SCUBA tank. Depending on the availability, 4–7 samples were collected from each of the five massive coral species studied. The collection permit from the Puerto Rico Department of Natural and Environmental Resources limited our sampling to ≤ 7 samples per species. The scar left by the drilling was covered with hydraulic cement to avoid colonization of turf algae or any other opportunistic organism. All the samples were transported in aerated seawater in sterilized Whirl-Pak plastic bags and transported to an outdoor aquarium located at the University of Puerto Rico’s Department of Marine Sciences Magueyes Island Field Station (UPR) for immediate processing. The whole transportation process took approximately 30–40 minutes.

Bottom Line: We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments.Additionally, pigments typically found in endolithic algae were also identified.Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

View Article: PubMed Central - PubMed

Affiliation: Bay Area Environmental Research Institute/NASA Ames Research Center, MS 245-4, Bldg 245, Rm. 120, Moffett Field, CA, 94035, United States of America.

ABSTRACT
Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

Show MeSH
Related in: MedlinePlus