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Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Related in: MedlinePlus

Fabrication and operation of a multilayer microfluidic device (MMD) for efficient culture and analysis of renal tubular cells. (A) Sandwich structure of MMD. Photograph (B) and schematic of the device (C) on a culture dish containing outside tubular fluid. (D) Microscope image of IMCD cells grown confluently after seeding three days within the MMD. Reproduced from Jang KJ, Suh KY. A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells. Lab Chip. 2010;10(1):36–42, with permission from the Royal Society of Chemistry.
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f6-aci-10-2015-039: Fabrication and operation of a multilayer microfluidic device (MMD) for efficient culture and analysis of renal tubular cells. (A) Sandwich structure of MMD. Photograph (B) and schematic of the device (C) on a culture dish containing outside tubular fluid. (D) Microscope image of IMCD cells grown confluently after seeding three days within the MMD. Reproduced from Jang KJ, Suh KY. A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells. Lab Chip. 2010;10(1):36–42, with permission from the Royal Society of Chemistry.

Mentions: There were some early works exploring on nephrotoxicity screening platform-based microfluidics. With the similar PDMS champing porous membrane structure, Jang and Suh used rats, collecting duct epithelial cells to build kidney tubules on chip and observed their transportation ability on the chip40 (Fig. 6). In an independent report, Ferrell used opossum kidney epithelial cells to build the chip and analyzed the mechanism of albumin transportation.41 Also, Jang KJ et al applied human primary renal tubules epithelial cells on their microfluidic device and measured a number of important parameters, such as albumin transport, glucose reabsorption, brush border alkaline phosphatase activity, cisplatin toxicity, and P-gp efflux transporter activity42 on chip. All of these works used only one kind of cells in forming partial physiological environment, and then characterized their systems with a series of biomarkers.


Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Fabrication and operation of a multilayer microfluidic device (MMD) for efficient culture and analysis of renal tubular cells. (A) Sandwich structure of MMD. Photograph (B) and schematic of the device (C) on a culture dish containing outside tubular fluid. (D) Microscope image of IMCD cells grown confluently after seeding three days within the MMD. Reproduced from Jang KJ, Suh KY. A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells. Lab Chip. 2010;10(1):36–42, with permission from the Royal Society of Chemistry.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664205&req=5

f6-aci-10-2015-039: Fabrication and operation of a multilayer microfluidic device (MMD) for efficient culture and analysis of renal tubular cells. (A) Sandwich structure of MMD. Photograph (B) and schematic of the device (C) on a culture dish containing outside tubular fluid. (D) Microscope image of IMCD cells grown confluently after seeding three days within the MMD. Reproduced from Jang KJ, Suh KY. A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells. Lab Chip. 2010;10(1):36–42, with permission from the Royal Society of Chemistry.
Mentions: There were some early works exploring on nephrotoxicity screening platform-based microfluidics. With the similar PDMS champing porous membrane structure, Jang and Suh used rats, collecting duct epithelial cells to build kidney tubules on chip and observed their transportation ability on the chip40 (Fig. 6). In an independent report, Ferrell used opossum kidney epithelial cells to build the chip and analyzed the mechanism of albumin transportation.41 Also, Jang KJ et al applied human primary renal tubules epithelial cells on their microfluidic device and measured a number of important parameters, such as albumin transport, glucose reabsorption, brush border alkaline phosphatase activity, cisplatin toxicity, and P-gp efflux transporter activity42 on chip. All of these works used only one kind of cells in forming partial physiological environment, and then characterized their systems with a series of biomarkers.

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Related in: MedlinePlus