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Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Related in: MedlinePlus

Experimental models to study MGE neurons migration from the explants of mouse embryos. (A) Brains of the E15 embryos isolated from female mice and sliced to dissect MGE explants (B). MGE explants were placed in a Matrigel coated glass dish (C) and neurons migrated out after 48 hours (D). MGE explants and neurons were costained with neuronal marker βIII-tubulin (red), centromere marker pericentrin (green), and the nuclear marker ToPro3(blue) (E). MGE explant and cortical explant were placed in one of the wells of a microfluidic device, respectively (F). Neurons migrated away from MGE explant toward the cortical explant within microchannels filled with Matrigel (G–I). Reproduced from Nery FC, da Hora CC, Yaqub U, et al. New methods for investigation of neuronal migration in embryonic brain explants. J Neurosci Methods. 2015;239:80–84, with permission from Elsevier.
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Related In: Results  -  Collection


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f5-aci-10-2015-039: Experimental models to study MGE neurons migration from the explants of mouse embryos. (A) Brains of the E15 embryos isolated from female mice and sliced to dissect MGE explants (B). MGE explants were placed in a Matrigel coated glass dish (C) and neurons migrated out after 48 hours (D). MGE explants and neurons were costained with neuronal marker βIII-tubulin (red), centromere marker pericentrin (green), and the nuclear marker ToPro3(blue) (E). MGE explant and cortical explant were placed in one of the wells of a microfluidic device, respectively (F). Neurons migrated away from MGE explant toward the cortical explant within microchannels filled with Matrigel (G–I). Reproduced from Nery FC, da Hora CC, Yaqub U, et al. New methods for investigation of neuronal migration in embryonic brain explants. J Neurosci Methods. 2015;239:80–84, with permission from Elsevier.

Mentions: On a microfluidic device fabricated by Shamloo and coworkers,37 rat NSPCs were isolated and cultured within varying densities of collagen matrices, and then, the effect of varying concentrations of nerve growth factor on migration and differentiation was detected.37 Moreover, a method for observing migration out of the embryonic medial ganglionic eminence (MGE) in real-time was described38 (Fig. 5). Live neurons were costained with different intracellular probes to monitor the distribution of cellular organelles, including Golgi and nuclear. Then, an efficient method to transfect cells in MGE explants with adeno-associated virus expressing reporter proteins was established. In another work, NSPCs were cultured on an indium tin oxide surface, and cell differentiation, process development, and functionality of differentiated neuron were successfully controlled by the electrical stimulation.39


Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Experimental models to study MGE neurons migration from the explants of mouse embryos. (A) Brains of the E15 embryos isolated from female mice and sliced to dissect MGE explants (B). MGE explants were placed in a Matrigel coated glass dish (C) and neurons migrated out after 48 hours (D). MGE explants and neurons were costained with neuronal marker βIII-tubulin (red), centromere marker pericentrin (green), and the nuclear marker ToPro3(blue) (E). MGE explant and cortical explant were placed in one of the wells of a microfluidic device, respectively (F). Neurons migrated away from MGE explant toward the cortical explant within microchannels filled with Matrigel (G–I). Reproduced from Nery FC, da Hora CC, Yaqub U, et al. New methods for investigation of neuronal migration in embryonic brain explants. J Neurosci Methods. 2015;239:80–84, with permission from Elsevier.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664205&req=5

f5-aci-10-2015-039: Experimental models to study MGE neurons migration from the explants of mouse embryos. (A) Brains of the E15 embryos isolated from female mice and sliced to dissect MGE explants (B). MGE explants were placed in a Matrigel coated glass dish (C) and neurons migrated out after 48 hours (D). MGE explants and neurons were costained with neuronal marker βIII-tubulin (red), centromere marker pericentrin (green), and the nuclear marker ToPro3(blue) (E). MGE explant and cortical explant were placed in one of the wells of a microfluidic device, respectively (F). Neurons migrated away from MGE explant toward the cortical explant within microchannels filled with Matrigel (G–I). Reproduced from Nery FC, da Hora CC, Yaqub U, et al. New methods for investigation of neuronal migration in embryonic brain explants. J Neurosci Methods. 2015;239:80–84, with permission from Elsevier.
Mentions: On a microfluidic device fabricated by Shamloo and coworkers,37 rat NSPCs were isolated and cultured within varying densities of collagen matrices, and then, the effect of varying concentrations of nerve growth factor on migration and differentiation was detected.37 Moreover, a method for observing migration out of the embryonic medial ganglionic eminence (MGE) in real-time was described38 (Fig. 5). Live neurons were costained with different intracellular probes to monitor the distribution of cellular organelles, including Golgi and nuclear. Then, an efficient method to transfect cells in MGE explants with adeno-associated virus expressing reporter proteins was established. In another work, NSPCs were cultured on an indium tin oxide surface, and cell differentiation, process development, and functionality of differentiated neuron were successfully controlled by the electrical stimulation.39

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Related in: MedlinePlus