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Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Microfluidic encapsulation of hepatocyte pucks. (A) A microtissue of hepatocyte pucks and fibroblast were generated using a (B) microfluidic droplet generating device. (C) Phase image and viability staining of an individual microtissue. (D) Albumin secretion of different kinds of microtissue in 16 days. (E) Coculture array of microtissues seeded together onto micropatterned islands. Reproduced with permission from Li CY, Stevens KR, Schwartz RE, Alejandro BS, Huang JH, Bhatia SN. Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues. Tissue Eng Part A. 2014;20(15–16):2200–2212. The publisher for this copyrighted material is Mary Ann Liebert, Inc. publishers.
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Related In: Results  -  Collection


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f4-aci-10-2015-039: Microfluidic encapsulation of hepatocyte pucks. (A) A microtissue of hepatocyte pucks and fibroblast were generated using a (B) microfluidic droplet generating device. (C) Phase image and viability staining of an individual microtissue. (D) Albumin secretion of different kinds of microtissue in 16 days. (E) Coculture array of microtissues seeded together onto micropatterned islands. Reproduced with permission from Li CY, Stevens KR, Schwartz RE, Alejandro BS, Huang JH, Bhatia SN. Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues. Tissue Eng Part A. 2014;20(15–16):2200–2212. The publisher for this copyrighted material is Mary Ann Liebert, Inc. publishers.

Mentions: With the development of lab-on-a-chip technique, it has been demonstrated that a continuous medium flow allows a feasible exchange of oxygen and wastes, respectively, with the outside and creates a more physiological environment in stimulating the expression of detoxifying genes.14 Moreover, with a liver-on-a-chip method, appropriate interaction between cell–cell, cell–ECM, and cell-soluble factors contribute to stabilize primary hepatocyte functions.15,16 Also, micro-bioreactor based on hepatocytes is considered to be a promising in vitro model for artificial liver, in which hepatocytes were 3D cultured in different approaches, such as spheroids, sandwich gels, porous scaffolds, or encapsulation in natural or synthetic hydrogels.17–26 In another report, rat hepatocytes were isolated by Li and coworkers, and these cells were then encapsulated in polyethylene glycol diacrylate, which maintained liver-specific function for over 50 days while responding to prototypic small molecules and drug–drug interactions. Even more, this microtissue could be manufactured using a microfluidic droplet generator27 (Fig. 4).


Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Microfluidic encapsulation of hepatocyte pucks. (A) A microtissue of hepatocyte pucks and fibroblast were generated using a (B) microfluidic droplet generating device. (C) Phase image and viability staining of an individual microtissue. (D) Albumin secretion of different kinds of microtissue in 16 days. (E) Coculture array of microtissues seeded together onto micropatterned islands. Reproduced with permission from Li CY, Stevens KR, Schwartz RE, Alejandro BS, Huang JH, Bhatia SN. Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues. Tissue Eng Part A. 2014;20(15–16):2200–2212. The publisher for this copyrighted material is Mary Ann Liebert, Inc. publishers.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664205&req=5

f4-aci-10-2015-039: Microfluidic encapsulation of hepatocyte pucks. (A) A microtissue of hepatocyte pucks and fibroblast were generated using a (B) microfluidic droplet generating device. (C) Phase image and viability staining of an individual microtissue. (D) Albumin secretion of different kinds of microtissue in 16 days. (E) Coculture array of microtissues seeded together onto micropatterned islands. Reproduced with permission from Li CY, Stevens KR, Schwartz RE, Alejandro BS, Huang JH, Bhatia SN. Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues. Tissue Eng Part A. 2014;20(15–16):2200–2212. The publisher for this copyrighted material is Mary Ann Liebert, Inc. publishers.
Mentions: With the development of lab-on-a-chip technique, it has been demonstrated that a continuous medium flow allows a feasible exchange of oxygen and wastes, respectively, with the outside and creates a more physiological environment in stimulating the expression of detoxifying genes.14 Moreover, with a liver-on-a-chip method, appropriate interaction between cell–cell, cell–ECM, and cell-soluble factors contribute to stabilize primary hepatocyte functions.15,16 Also, micro-bioreactor based on hepatocytes is considered to be a promising in vitro model for artificial liver, in which hepatocytes were 3D cultured in different approaches, such as spheroids, sandwich gels, porous scaffolds, or encapsulation in natural or synthetic hydrogels.17–26 In another report, rat hepatocytes were isolated by Li and coworkers, and these cells were then encapsulated in polyethylene glycol diacrylate, which maintained liver-specific function for over 50 days while responding to prototypic small molecules and drug–drug interactions. Even more, this microtissue could be manufactured using a microfluidic droplet generator27 (Fig. 4).

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.