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Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Related in: MedlinePlus

Microfluidic device design and workflow to analyze biomolecular signaling in angiogenesis. (A) Macrophages and the HUVEC/Primary neonatal normal human dermal fibroblast (NHDF) mixture were seeded in left and right channels, respectively. (B) An array of 14 microculture devices. (C) Workflow showing cell seeding, polarization, and ICC. Reprinted with permission from Theberge AB, Yu J, Young EW, Ricke WA, Bushman W, Beebe DJ. Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis. Anal Chem. 2015;87(6):3239–3246. Copyright 2015 American Chemical Society.
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Related In: Results  -  Collection


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f3-aci-10-2015-039: Microfluidic device design and workflow to analyze biomolecular signaling in angiogenesis. (A) Macrophages and the HUVEC/Primary neonatal normal human dermal fibroblast (NHDF) mixture were seeded in left and right channels, respectively. (B) An array of 14 microculture devices. (C) Workflow showing cell seeding, polarization, and ICC. Reprinted with permission from Theberge AB, Yu J, Young EW, Ricke WA, Bushman W, Beebe DJ. Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis. Anal Chem. 2015;87(6):3239–3246. Copyright 2015 American Chemical Society.

Mentions: A functional and perfusable 3D microvascular network on a chip was reported recently7 (Fig. 2). In that report, ECs were spatially controlled cocultured with stromal fibroblasts, pericytes, or cancer cells to generate normal microvessel development and tumor vasculature angiogenesis. Further research in the regulating angiogenesis mechanism was achieved. It was realized with the powerful microfluidic system that incorporated the whole milieu of soluble factors, produced by cells in situ and allowed potential drugs to target angiogenesis in disease8 (Fig. 3).


Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Microfluidic device design and workflow to analyze biomolecular signaling in angiogenesis. (A) Macrophages and the HUVEC/Primary neonatal normal human dermal fibroblast (NHDF) mixture were seeded in left and right channels, respectively. (B) An array of 14 microculture devices. (C) Workflow showing cell seeding, polarization, and ICC. Reprinted with permission from Theberge AB, Yu J, Young EW, Ricke WA, Bushman W, Beebe DJ. Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis. Anal Chem. 2015;87(6):3239–3246. Copyright 2015 American Chemical Society.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664205&req=5

f3-aci-10-2015-039: Microfluidic device design and workflow to analyze biomolecular signaling in angiogenesis. (A) Macrophages and the HUVEC/Primary neonatal normal human dermal fibroblast (NHDF) mixture were seeded in left and right channels, respectively. (B) An array of 14 microculture devices. (C) Workflow showing cell seeding, polarization, and ICC. Reprinted with permission from Theberge AB, Yu J, Young EW, Ricke WA, Bushman W, Beebe DJ. Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis. Anal Chem. 2015;87(6):3239–3246. Copyright 2015 American Chemical Society.
Mentions: A functional and perfusable 3D microvascular network on a chip was reported recently7 (Fig. 2). In that report, ECs were spatially controlled cocultured with stromal fibroblasts, pericytes, or cancer cells to generate normal microvessel development and tumor vasculature angiogenesis. Further research in the regulating angiogenesis mechanism was achieved. It was realized with the powerful microfluidic system that incorporated the whole milieu of soluble factors, produced by cells in situ and allowed potential drugs to target angiogenesis in disease8 (Fig. 3).

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


Related in: MedlinePlus