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Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.


SEM image for the thick layer of cardiac tissue attached to the tubing surface after remodeling. Primary and hESC-derived cardiomyocytes were used to generate cardiac biowires and beat spontaneously. Reproduced from Xiao Y, Zhang B, Liu H, et al. Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle. Lab Chip. 2014;14(5):869–882, with permission from the Royal Society of Chemistry.
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Related In: Results  -  Collection


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f1-aci-10-2015-039: SEM image for the thick layer of cardiac tissue attached to the tubing surface after remodeling. Primary and hESC-derived cardiomyocytes were used to generate cardiac biowires and beat spontaneously. Reproduced from Xiao Y, Zhang B, Liu H, et al. Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle. Lab Chip. 2014;14(5):869–882, with permission from the Royal Society of Chemistry.

Mentions: In Xiao’s report,4 human embryonic stem cell (hESC) and primary neonatal rat cardiomyocytes were used to generate a microfabricated cardiac biowire bioreactor that was attractive for pharmaceutical testing. The spontaneous beating of the cardiac biowires could be retarded by the treatment of nitric oxide, which was carried by the medium into the chamber. Furthermore, the integrated carbon rod electrodes offered electrical stimulation for further improving the phenotype of cardiomyocytes (Fig. 1). Nguyen and colleagues reported a heart-on-chip methodology with which an accurate controllable physiologic mechanical stimulation was managed to rebuild a simulative physiological condition that holds significant promise for immature cardiomyocytes for generating functional cardiac patches in vitro for replacement of injured cardiac tissues.5 In another example,6 a microfluidic device that generates cardiac-like flow in a continuous closed culture system was reported with advantages of miniaturization, low circulatory volume (2–3 μL).


Organ-on-a-Chip: New Platform for Biological Analysis.

An F, Qu Y, Liu X, Zhong R, Luo Y - Anal Chem Insights (2015)

SEM image for the thick layer of cardiac tissue attached to the tubing surface after remodeling. Primary and hESC-derived cardiomyocytes were used to generate cardiac biowires and beat spontaneously. Reproduced from Xiao Y, Zhang B, Liu H, et al. Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle. Lab Chip. 2014;14(5):869–882, with permission from the Royal Society of Chemistry.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664205&req=5

f1-aci-10-2015-039: SEM image for the thick layer of cardiac tissue attached to the tubing surface after remodeling. Primary and hESC-derived cardiomyocytes were used to generate cardiac biowires and beat spontaneously. Reproduced from Xiao Y, Zhang B, Liu H, et al. Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle. Lab Chip. 2014;14(5):869–882, with permission from the Royal Society of Chemistry.
Mentions: In Xiao’s report,4 human embryonic stem cell (hESC) and primary neonatal rat cardiomyocytes were used to generate a microfabricated cardiac biowire bioreactor that was attractive for pharmaceutical testing. The spontaneous beating of the cardiac biowires could be retarded by the treatment of nitric oxide, which was carried by the medium into the chamber. Furthermore, the integrated carbon rod electrodes offered electrical stimulation for further improving the phenotype of cardiomyocytes (Fig. 1). Nguyen and colleagues reported a heart-on-chip methodology with which an accurate controllable physiologic mechanical stimulation was managed to rebuild a simulative physiological condition that holds significant promise for immature cardiomyocytes for generating functional cardiac patches in vitro for replacement of injured cardiac tissues.5 In another example,6 a microfluidic device that generates cardiac-like flow in a continuous closed culture system was reported with advantages of miniaturization, low circulatory volume (2–3 μL).

Bottom Line: Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy.The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip".In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China. ; State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.

ABSTRACT
Direct detection and analysis of biomolecules and cells in physiological microenvironment is urgently needed for fast evaluation of biology and pharmacy. The past several years have witnessed remarkable development opportunities in vitro organs and tissues models with multiple functions based on microfluidic devices, termed as "organ-on-a-chip". Briefly speaking, it is a promising technology in rebuilding physiological functions of tissues and organs, featuring mammalian cell co-culture and artificial microenvironment created by microchannel networks. In this review, we summarized the advances in studies of heart-, vessel-, liver-, neuron-, kidney- and Multi-organs-on-a-chip, and discussed some noteworthy potential on-chip detection schemes.

No MeSH data available.