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Antibacterial and Toxic Effect of Hydrogen Peroxide Combined with Different Concentrations of Chlorhexidine in Comparison with Sodium Hypochlorite.

Mirhadi H, Abbaszadegan A, Ranjbar MA, Azar MR, Geramizadeh B, Torabi S, Sadat Aleyasin Z, Gholami A - J Dent (Shiraz) (2015)

Bottom Line: The cytocompatibility and antibacterial activity of 0.1, 0.2, 1 and 2% CHX combined with 3% H2O2 were evaluated and compared with 5.25 and 2.5% NaOCl.The viability of PDL cells was lower when treated with 5.25 or 2.5% NaOCl than all combinations of CHX and H2O2.There was no significant difference in the antibacterial activity of the solutions against E.faecalis, except for the 0.1% CHX + 3% H2O2 combination, which had significantly lower efficacy than other groups.All combinations of CHX and H2O2 (used in this study)except 0.1% CHX + 3% H2O2 were efficient irrigants against planktonic E.faecalis and had a better cytocompatibility with PDL cells than 5.25 and 2.5% NaOCl.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Endodontics, School of Dentistry, Shiraz University of Medical Sciences; Shiraz, Iran.

ABSTRACT

Statement of the problem: Hydrogen peroxide (H2O2) has been suggested to be used in sequence or in combination with chlorhexidine (CHX) to enhance the antibacterial activity against Enterococcus faecalis, but there is no research in the literature on the safety and effectiveness of this irrigation protocol.

Purpose: This study aimed to assess the cytocompatibility and antibacterial activity of different concentrations of CHX combined with H2O2in comparison with the activity of 5.25 and 2.5% sodium hypochlorite (NaOCl).

Materials and method: Different concentrations of H2O2 (10, 5, 3 and 1%) were exposed to the PDL cells. Then, the solution with minimal cytotoxicity was selected (3% H2O2). The cytocompatibility and antibacterial activity of 0.1, 0.2, 1 and 2% CHX combined with 3% H2O2 were evaluated and compared with 5.25 and 2.5% NaOCl. The differences in the mean viability of PDL cells were evaluated by one-way ANOVA. Kruskal-Wallis and post-hoc Dunn's tests were adopted to compare the antibacterial activity of the solutions against E.faecalis.

Results: The viability of PDL cells was lower when treated with 5.25 or 2.5% NaOCl than all combinations of CHX and H2O2.There was no significant difference in the antibacterial activity of the solutions against E.faecalis, except for the 0.1% CHX + 3% H2O2 combination, which had significantly lower efficacy than other groups.

Conclusion: All combinations of CHX and H2O2 (used in this study)except 0.1% CHX + 3% H2O2 were efficient irrigants against planktonic E.faecalis and had a better cytocompatibility with PDL cells than 5.25 and 2.5% NaOCl.

No MeSH data available.


The mean cell viability of the PDL fibroblasts treated with different experimental solutions. Having equal letters denotes a lack of statistically significant difference (p> 0.05)
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Figure 1: The mean cell viability of the PDL fibroblasts treated with different experimental solutions. Having equal letters denotes a lack of statistically significant difference (p> 0.05)

Mentions: All the tested solutions had a negative effect on the viability of PDL cells after 20 min of contact. It was found that 35% H2O2, as positive control, caused 98.16% cellular death. The mean cell viability values of PDL cells in the first and second phase of the study are summarized in Table 1 and Figure 1. Statistical analyses showed that the mean cell viability of 5.25% and 2.5% NaOCl were significantly lower than all combinations of CHX and H2O2 exceptfor 2% CHX combined with 3% H2O2. Although the mixture of 2% CHX and 3% H2O2 was the most toxic combination of these two irrigants, its relevant cytocompatibility result was not significantly different from that of 5.25 and 2.5 % NaOCl.


Antibacterial and Toxic Effect of Hydrogen Peroxide Combined with Different Concentrations of Chlorhexidine in Comparison with Sodium Hypochlorite.

Mirhadi H, Abbaszadegan A, Ranjbar MA, Azar MR, Geramizadeh B, Torabi S, Sadat Aleyasin Z, Gholami A - J Dent (Shiraz) (2015)

The mean cell viability of the PDL fibroblasts treated with different experimental solutions. Having equal letters denotes a lack of statistically significant difference (p> 0.05)
© Copyright Policy
Related In: Results  -  Collection

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Figure 1: The mean cell viability of the PDL fibroblasts treated with different experimental solutions. Having equal letters denotes a lack of statistically significant difference (p> 0.05)
Mentions: All the tested solutions had a negative effect on the viability of PDL cells after 20 min of contact. It was found that 35% H2O2, as positive control, caused 98.16% cellular death. The mean cell viability values of PDL cells in the first and second phase of the study are summarized in Table 1 and Figure 1. Statistical analyses showed that the mean cell viability of 5.25% and 2.5% NaOCl were significantly lower than all combinations of CHX and H2O2 exceptfor 2% CHX combined with 3% H2O2. Although the mixture of 2% CHX and 3% H2O2 was the most toxic combination of these two irrigants, its relevant cytocompatibility result was not significantly different from that of 5.25 and 2.5 % NaOCl.

Bottom Line: The cytocompatibility and antibacterial activity of 0.1, 0.2, 1 and 2% CHX combined with 3% H2O2 were evaluated and compared with 5.25 and 2.5% NaOCl.The viability of PDL cells was lower when treated with 5.25 or 2.5% NaOCl than all combinations of CHX and H2O2.There was no significant difference in the antibacterial activity of the solutions against E.faecalis, except for the 0.1% CHX + 3% H2O2 combination, which had significantly lower efficacy than other groups.All combinations of CHX and H2O2 (used in this study)except 0.1% CHX + 3% H2O2 were efficient irrigants against planktonic E.faecalis and had a better cytocompatibility with PDL cells than 5.25 and 2.5% NaOCl.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Endodontics, School of Dentistry, Shiraz University of Medical Sciences; Shiraz, Iran.

ABSTRACT

Statement of the problem: Hydrogen peroxide (H2O2) has been suggested to be used in sequence or in combination with chlorhexidine (CHX) to enhance the antibacterial activity against Enterococcus faecalis, but there is no research in the literature on the safety and effectiveness of this irrigation protocol.

Purpose: This study aimed to assess the cytocompatibility and antibacterial activity of different concentrations of CHX combined with H2O2in comparison with the activity of 5.25 and 2.5% sodium hypochlorite (NaOCl).

Materials and method: Different concentrations of H2O2 (10, 5, 3 and 1%) were exposed to the PDL cells. Then, the solution with minimal cytotoxicity was selected (3% H2O2). The cytocompatibility and antibacterial activity of 0.1, 0.2, 1 and 2% CHX combined with 3% H2O2 were evaluated and compared with 5.25 and 2.5% NaOCl. The differences in the mean viability of PDL cells were evaluated by one-way ANOVA. Kruskal-Wallis and post-hoc Dunn's tests were adopted to compare the antibacterial activity of the solutions against E.faecalis.

Results: The viability of PDL cells was lower when treated with 5.25 or 2.5% NaOCl than all combinations of CHX and H2O2.There was no significant difference in the antibacterial activity of the solutions against E.faecalis, except for the 0.1% CHX + 3% H2O2 combination, which had significantly lower efficacy than other groups.

Conclusion: All combinations of CHX and H2O2 (used in this study)except 0.1% CHX + 3% H2O2 were efficient irrigants against planktonic E.faecalis and had a better cytocompatibility with PDL cells than 5.25 and 2.5% NaOCl.

No MeSH data available.