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P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas.

Jaafari-Ashkavandi Z, Geramizadeh B, Ranjbar MA - J Dent (Shiraz) (2015)

Bottom Line: Its expression with Ki-67 proliferation marker was also compared.There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Statement of the problem: P63 gene is a member of TP53 and its homologous gene family. Its expression was observed in some odontogenic lesions, more expression in aggressive lesions.

Purpose: This study aimed to investigate the possible diagnostic impact of P63 protein on dentigerous cysts and various types of ameloblastoma. Its expression with Ki-67 proliferation marker was also compared.

Materials and method: This cross-sectional retrospective study was enrolled on 25 cases of dentigerous cyst including 21 unicystic ameloblastomas and 17 conventional ameloblastomas. The expression of P63 and Ki-67 was assessed by immunohistochemical (IHC) examinations. Data were analyzed by employing Mann-Whitney and correlation coefficient tests.

Results: P63 expression was significantly higher in ameloblastoma than unicystic ameloblastoma and dentigerous cysts. There was no significant difference between unicystic ameloblastoma and dentigerous cyst in P63 expression. A 90% cut-off point was obtained for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions from others. There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.

Conclusion: More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63. Moreover, the expression of P63 protein had not any correlation with Ki-67 protein in dentigerous cysts and ameloblastomas.

No MeSH data available.


Related in: MedlinePlus

ROC curve for basal and superficial layers: evaluation of basal layer is better for differentiation of aggressive lesions
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Figure 3: ROC curve for basal and superficial layers: evaluation of basal layer is better for differentiation of aggressive lesions

Mentions: According to the receiver operating characteristic (ROC) curve analysis, we obtained a 90% cut-off point for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions including mural and solid ameloblast from others (dentigerous and luminal). The area under ROC curve was 0.84 with 95% confidence interval (CI). Cut-off point for suprabasal layers was 77% with 72% sensitivity and 77% specificity. The area under this curve was 0.75. This analysis demonstrated that basal layer was a better area than suprabasal layers to differentiate aggressiveness of the lesions (Figure 3).


P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas.

Jaafari-Ashkavandi Z, Geramizadeh B, Ranjbar MA - J Dent (Shiraz) (2015)

ROC curve for basal and superficial layers: evaluation of basal layer is better for differentiation of aggressive lesions
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664029&req=5

Figure 3: ROC curve for basal and superficial layers: evaluation of basal layer is better for differentiation of aggressive lesions
Mentions: According to the receiver operating characteristic (ROC) curve analysis, we obtained a 90% cut-off point for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions including mural and solid ameloblast from others (dentigerous and luminal). The area under ROC curve was 0.84 with 95% confidence interval (CI). Cut-off point for suprabasal layers was 77% with 72% sensitivity and 77% specificity. The area under this curve was 0.75. This analysis demonstrated that basal layer was a better area than suprabasal layers to differentiate aggressiveness of the lesions (Figure 3).

Bottom Line: Its expression with Ki-67 proliferation marker was also compared.There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Statement of the problem: P63 gene is a member of TP53 and its homologous gene family. Its expression was observed in some odontogenic lesions, more expression in aggressive lesions.

Purpose: This study aimed to investigate the possible diagnostic impact of P63 protein on dentigerous cysts and various types of ameloblastoma. Its expression with Ki-67 proliferation marker was also compared.

Materials and method: This cross-sectional retrospective study was enrolled on 25 cases of dentigerous cyst including 21 unicystic ameloblastomas and 17 conventional ameloblastomas. The expression of P63 and Ki-67 was assessed by immunohistochemical (IHC) examinations. Data were analyzed by employing Mann-Whitney and correlation coefficient tests.

Results: P63 expression was significantly higher in ameloblastoma than unicystic ameloblastoma and dentigerous cysts. There was no significant difference between unicystic ameloblastoma and dentigerous cyst in P63 expression. A 90% cut-off point was obtained for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions from others. There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.

Conclusion: More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63. Moreover, the expression of P63 protein had not any correlation with Ki-67 protein in dentigerous cysts and ameloblastomas.

No MeSH data available.


Related in: MedlinePlus