Limits...
P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas.

Jaafari-Ashkavandi Z, Geramizadeh B, Ranjbar MA - J Dent (Shiraz) (2015)

Bottom Line: Its expression with Ki-67 proliferation marker was also compared.There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Statement of the problem: P63 gene is a member of TP53 and its homologous gene family. Its expression was observed in some odontogenic lesions, more expression in aggressive lesions.

Purpose: This study aimed to investigate the possible diagnostic impact of P63 protein on dentigerous cysts and various types of ameloblastoma. Its expression with Ki-67 proliferation marker was also compared.

Materials and method: This cross-sectional retrospective study was enrolled on 25 cases of dentigerous cyst including 21 unicystic ameloblastomas and 17 conventional ameloblastomas. The expression of P63 and Ki-67 was assessed by immunohistochemical (IHC) examinations. Data were analyzed by employing Mann-Whitney and correlation coefficient tests.

Results: P63 expression was significantly higher in ameloblastoma than unicystic ameloblastoma and dentigerous cysts. There was no significant difference between unicystic ameloblastoma and dentigerous cyst in P63 expression. A 90% cut-off point was obtained for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions from others. There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.

Conclusion: More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63. Moreover, the expression of P63 protein had not any correlation with Ki-67 protein in dentigerous cysts and ameloblastomas.

No MeSH data available.


Related in: MedlinePlus

a: P63 expression in ameloblastoma (×400 magnification). b: Squamous metaplasia does not show P63 expression.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664029&req=5

Figure 2: a: P63 expression in ameloblastoma (×400 magnification). b: Squamous metaplasia does not show P63 expression.

Mentions: The unicystic ameloblastomas consisted of 4 cases of luminal and 17 cases of mural types. The pattern of P63 expression was similar to dentigerous cysts. Basal layer showed more positive cells; however, the intensity of staining was higher (Table 2, Figure 1b, c). There was no significant difference between the cystic lining of luminal and mural ameloblastomas (p= 0.38 by Mann-Whitney test). The ameloblastoma group included 10 follicular and 7 plexiform subtypes. P63 positive cells were found in most peripheral and central cells of ameloblastic nests and in both histological subtypes (Table 1, Figure 2a). However, squamous metaplasia and lining of microcysts were not labeled (Figure 2b). Follicular and plexiform ameloblastomas did not show significant difference in P63 expression.


P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas.

Jaafari-Ashkavandi Z, Geramizadeh B, Ranjbar MA - J Dent (Shiraz) (2015)

a: P63 expression in ameloblastoma (×400 magnification). b: Squamous metaplasia does not show P63 expression.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664029&req=5

Figure 2: a: P63 expression in ameloblastoma (×400 magnification). b: Squamous metaplasia does not show P63 expression.
Mentions: The unicystic ameloblastomas consisted of 4 cases of luminal and 17 cases of mural types. The pattern of P63 expression was similar to dentigerous cysts. Basal layer showed more positive cells; however, the intensity of staining was higher (Table 2, Figure 1b, c). There was no significant difference between the cystic lining of luminal and mural ameloblastomas (p= 0.38 by Mann-Whitney test). The ameloblastoma group included 10 follicular and 7 plexiform subtypes. P63 positive cells were found in most peripheral and central cells of ameloblastic nests and in both histological subtypes (Table 1, Figure 2a). However, squamous metaplasia and lining of microcysts were not labeled (Figure 2b). Follicular and plexiform ameloblastomas did not show significant difference in P63 expression.

Bottom Line: Its expression with Ki-67 proliferation marker was also compared.There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Statement of the problem: P63 gene is a member of TP53 and its homologous gene family. Its expression was observed in some odontogenic lesions, more expression in aggressive lesions.

Purpose: This study aimed to investigate the possible diagnostic impact of P63 protein on dentigerous cysts and various types of ameloblastoma. Its expression with Ki-67 proliferation marker was also compared.

Materials and method: This cross-sectional retrospective study was enrolled on 25 cases of dentigerous cyst including 21 unicystic ameloblastomas and 17 conventional ameloblastomas. The expression of P63 and Ki-67 was assessed by immunohistochemical (IHC) examinations. Data were analyzed by employing Mann-Whitney and correlation coefficient tests.

Results: P63 expression was significantly higher in ameloblastoma than unicystic ameloblastoma and dentigerous cysts. There was no significant difference between unicystic ameloblastoma and dentigerous cyst in P63 expression. A 90% cut-off point was obtained for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions from others. There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.

Conclusion: More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63. Moreover, the expression of P63 protein had not any correlation with Ki-67 protein in dentigerous cysts and ameloblastomas.

No MeSH data available.


Related in: MedlinePlus