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P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas.

Jaafari-Ashkavandi Z, Geramizadeh B, Ranjbar MA - J Dent (Shiraz) (2015)

Bottom Line: Its expression with Ki-67 proliferation marker was also compared.There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Statement of the problem: P63 gene is a member of TP53 and its homologous gene family. Its expression was observed in some odontogenic lesions, more expression in aggressive lesions.

Purpose: This study aimed to investigate the possible diagnostic impact of P63 protein on dentigerous cysts and various types of ameloblastoma. Its expression with Ki-67 proliferation marker was also compared.

Materials and method: This cross-sectional retrospective study was enrolled on 25 cases of dentigerous cyst including 21 unicystic ameloblastomas and 17 conventional ameloblastomas. The expression of P63 and Ki-67 was assessed by immunohistochemical (IHC) examinations. Data were analyzed by employing Mann-Whitney and correlation coefficient tests.

Results: P63 expression was significantly higher in ameloblastoma than unicystic ameloblastoma and dentigerous cysts. There was no significant difference between unicystic ameloblastoma and dentigerous cyst in P63 expression. A 90% cut-off point was obtained for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions from others. There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.

Conclusion: More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63. Moreover, the expression of P63 protein had not any correlation with Ki-67 protein in dentigerous cysts and ameloblastomas.

No MeSH data available.


Related in: MedlinePlus

a: P63 expression in dentigerous cyst in only basal layer.  b, c: P63 expression in luminal (b) and mural (c) unicystic ameloblastoma. Intense immunostaining in lower layers (×400 magnification)
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Figure 1: a: P63 expression in dentigerous cyst in only basal layer.  b, c: P63 expression in luminal (b) and mural (c) unicystic ameloblastoma. Intense immunostaining in lower layers (×400 magnification)

Mentions: In dentigerous cyst group; P63- positive cells were mainly located in basal cell layer of epithelial lining and the layers above this area had a lower mean of expression (Figure 1a); however, Mann-Whitney test showed that this difference was not statistically significant (p= 0.3). Superficial cells did not show any staining. The mean of P63 expression and its intensity are summarized in tables 1 and 2. There were 5 cases with mild to moderate inflammation that showed a slightly lower P63-expression, but it was not significantly different with non-inflamed cysts (p> 0.05).


P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas.

Jaafari-Ashkavandi Z, Geramizadeh B, Ranjbar MA - J Dent (Shiraz) (2015)

a: P63 expression in dentigerous cyst in only basal layer.  b, c: P63 expression in luminal (b) and mural (c) unicystic ameloblastoma. Intense immunostaining in lower layers (×400 magnification)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664029&req=5

Figure 1: a: P63 expression in dentigerous cyst in only basal layer.  b, c: P63 expression in luminal (b) and mural (c) unicystic ameloblastoma. Intense immunostaining in lower layers (×400 magnification)
Mentions: In dentigerous cyst group; P63- positive cells were mainly located in basal cell layer of epithelial lining and the layers above this area had a lower mean of expression (Figure 1a); however, Mann-Whitney test showed that this difference was not statistically significant (p= 0.3). Superficial cells did not show any staining. The mean of P63 expression and its intensity are summarized in tables 1 and 2. There were 5 cases with mild to moderate inflammation that showed a slightly lower P63-expression, but it was not significantly different with non-inflamed cysts (p> 0.05).

Bottom Line: Its expression with Ki-67 proliferation marker was also compared.There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Statement of the problem: P63 gene is a member of TP53 and its homologous gene family. Its expression was observed in some odontogenic lesions, more expression in aggressive lesions.

Purpose: This study aimed to investigate the possible diagnostic impact of P63 protein on dentigerous cysts and various types of ameloblastoma. Its expression with Ki-67 proliferation marker was also compared.

Materials and method: This cross-sectional retrospective study was enrolled on 25 cases of dentigerous cyst including 21 unicystic ameloblastomas and 17 conventional ameloblastomas. The expression of P63 and Ki-67 was assessed by immunohistochemical (IHC) examinations. Data were analyzed by employing Mann-Whitney and correlation coefficient tests.

Results: P63 expression was significantly higher in ameloblastoma than unicystic ameloblastoma and dentigerous cysts. There was no significant difference between unicystic ameloblastoma and dentigerous cyst in P63 expression. A 90% cut-off point was obtained for basal layer which gave 88% sensitivity and 78% specificity to distinguish more invasive lesions from others. There was not any correlation between P63 and Ki-67 immunostaining in the three study groups.

Conclusion: More aggressiveness and more invasiveness of odontogenic lesions depicted higher rate and also more intensive expression of P63. Moreover, the expression of P63 protein had not any correlation with Ki-67 protein in dentigerous cysts and ameloblastomas.

No MeSH data available.


Related in: MedlinePlus