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Cadmium affects the mitochondrial viability and the acid soluble thiols concentration in liver, kidney, heart and gills of Ancistrus brevifilis (Eigenmann, 1920).

Velasquez-Vottelerd P, Anton Y, Salazar-Lugo R - Open Vet J (2015)

Bottom Line: The freshwater fish Ancistrus brevifilis, which is found in Venezuelan rivers, is considered a potential sentinel fish in ecotoxicological studies.We also detected a significant decrease in AST levels was in fishes exposed to Cd for 7 days in liver and kidney tissues; these results suggests that AST levels are elevated in some tissues may act as cytoprotective and adaptive alternative mechanism related to the ROS detoxification, maintenance redox status and mitochondrial viability.We conclude that the Cd exposure effect on AST levels and MV, vary across fish tissues and is related to the exposure duration, the molecule dynamics in different tissues, the organism and environmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de proteínas e inmunotoxicidad, Postgrado de Biología Aplicada, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.

ABSTRACT
The freshwater fish Ancistrus brevifilis, which is found in Venezuelan rivers, is considered a potential sentinel fish in ecotoxicological studies. The cadmium (Cd) effect on the mitochondrial viability (MV) and acid soluble thiols levels (AST) in A. brevifilis tissues (liver, kidney, heart, and gill) was evaluated. Forty-two fish with similar sizes and weights were randomly selected, of which 7 fish (with their respective replicate) were exposed for 7 and 30 days to a Cd sublethal concentration (0.1 mg.l(-1)). We determined the MV through a Janus Green B colorimetric assay and we obtained the concentration of AST by Ellman's method. Mitochondrial viability decreased in fish exposed to Cd for 30 days with the liver being the most affected tissue. We also detected a significant decrease in AST levels was in fishes exposed to Cd for 7 days in liver and kidney tissues; these results suggests that AST levels are elevated in some tissues may act as cytoprotective and adaptive alternative mechanism related to the ROS detoxification, maintenance redox status and mitochondrial viability. Organ-specifics variations were observed in both assays. We conclude that the Cd exposure effect on AST levels and MV, vary across fish tissues and is related to the exposure duration, the molecule dynamics in different tissues, the organism and environmental conditions.

No MeSH data available.


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(a): Mitochondrial viability (%) of A. brevifilis liver 7 and 30 days of Cd exposure. (b): AST levels of A. brevifilis liver control, and 7 and 30 days Cd exposed. *: significant differences (P<0.05); **: Very significant differences (P<0.01); ***: Highly significant differences (P<0.001).
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Figure 1: (a): Mitochondrial viability (%) of A. brevifilis liver 7 and 30 days of Cd exposure. (b): AST levels of A. brevifilis liver control, and 7 and 30 days Cd exposed. *: significant differences (P<0.05); **: Very significant differences (P<0.01); ***: Highly significant differences (P<0.001).

Mentions: We detected a decrease in MV of hepatocytes by Cd exposure (P≤0.05), and the lowest values were observed in fish exposed for 30 d (Fig. 1a). We also observed a significant difference (P≤0.01) in AST distribution in the liver between exposed and unexposed fishes in both treatment groups. We detected lower AST concentrations in fishes in the 7d Cd exposed group compared to the controls and higher concentrations in the 30d Cd exposed group when compared to the controls (Fig. 1b).


Cadmium affects the mitochondrial viability and the acid soluble thiols concentration in liver, kidney, heart and gills of Ancistrus brevifilis (Eigenmann, 1920).

Velasquez-Vottelerd P, Anton Y, Salazar-Lugo R - Open Vet J (2015)

(a): Mitochondrial viability (%) of A. brevifilis liver 7 and 30 days of Cd exposure. (b): AST levels of A. brevifilis liver control, and 7 and 30 days Cd exposed. *: significant differences (P<0.05); **: Very significant differences (P<0.01); ***: Highly significant differences (P<0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663803&req=5

Figure 1: (a): Mitochondrial viability (%) of A. brevifilis liver 7 and 30 days of Cd exposure. (b): AST levels of A. brevifilis liver control, and 7 and 30 days Cd exposed. *: significant differences (P<0.05); **: Very significant differences (P<0.01); ***: Highly significant differences (P<0.001).
Mentions: We detected a decrease in MV of hepatocytes by Cd exposure (P≤0.05), and the lowest values were observed in fish exposed for 30 d (Fig. 1a). We also observed a significant difference (P≤0.01) in AST distribution in the liver between exposed and unexposed fishes in both treatment groups. We detected lower AST concentrations in fishes in the 7d Cd exposed group compared to the controls and higher concentrations in the 30d Cd exposed group when compared to the controls (Fig. 1b).

Bottom Line: The freshwater fish Ancistrus brevifilis, which is found in Venezuelan rivers, is considered a potential sentinel fish in ecotoxicological studies.We also detected a significant decrease in AST levels was in fishes exposed to Cd for 7 days in liver and kidney tissues; these results suggests that AST levels are elevated in some tissues may act as cytoprotective and adaptive alternative mechanism related to the ROS detoxification, maintenance redox status and mitochondrial viability.We conclude that the Cd exposure effect on AST levels and MV, vary across fish tissues and is related to the exposure duration, the molecule dynamics in different tissues, the organism and environmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de proteínas e inmunotoxicidad, Postgrado de Biología Aplicada, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.

ABSTRACT
The freshwater fish Ancistrus brevifilis, which is found in Venezuelan rivers, is considered a potential sentinel fish in ecotoxicological studies. The cadmium (Cd) effect on the mitochondrial viability (MV) and acid soluble thiols levels (AST) in A. brevifilis tissues (liver, kidney, heart, and gill) was evaluated. Forty-two fish with similar sizes and weights were randomly selected, of which 7 fish (with their respective replicate) were exposed for 7 and 30 days to a Cd sublethal concentration (0.1 mg.l(-1)). We determined the MV through a Janus Green B colorimetric assay and we obtained the concentration of AST by Ellman's method. Mitochondrial viability decreased in fish exposed to Cd for 30 days with the liver being the most affected tissue. We also detected a significant decrease in AST levels was in fishes exposed to Cd for 7 days in liver and kidney tissues; these results suggests that AST levels are elevated in some tissues may act as cytoprotective and adaptive alternative mechanism related to the ROS detoxification, maintenance redox status and mitochondrial viability. Organ-specifics variations were observed in both assays. We conclude that the Cd exposure effect on AST levels and MV, vary across fish tissues and is related to the exposure duration, the molecule dynamics in different tissues, the organism and environmental conditions.

No MeSH data available.


Related in: MedlinePlus