Limits...
Human Staphylococcus aureus lineages among Zoological Park residents in Greece.

Drougka E, Foka A, Posantzis D, Giormezis N, Anastassiou ED, Petinaki E, Spiliopoulou I - Open Vet J (2015)

Bottom Line: Clones were defined by Multilocus Sequence Typing (MLST), spa type and Pulsed-Field Gel Electrophoresis (PFGE).Seven S. aureus isolates were recovered from four animals and one from an employee.Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, University of Patras, Patras, Greece ; National Reference Laboratory for Staphylococci, University of Patras, Patras, Greece.

ABSTRACT
Staphylococcus aureus is a part of the microbiota flora in many animal species. The clonal spread of S. aureus among animals and personnel in a Zoological Park was investigated. Samples were collected from colonized and infected sites among 32 mammals, 11 birds and eight humans. The genes mecA, mecC, lukF/lukS-PV (encoding Panton-Valentine leukocidin, PVL) and tst (toxic shock syndrome toxin-1) were investigated by PCR. Clones were defined by Multilocus Sequence Typing (MLST), spa type and Pulsed-Field Gel Electrophoresis (PFGE). Seven S. aureus isolates were recovered from four animals and one from an employee. All were mecA, mecC and tst-negative, whereas, one carried the PVL genes and was isolated from an infected Squirrel monkey. Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin. Even though a variety of genotypes were identified among S. aureus strains colonizing zoo park residents, our results indicate that colonization with human lineages has indeed occurred.

No MeSH data available.


Related in: MedlinePlus

Dendogram of PFGE banding patterns of the isolated S. aureus strains and the respective MLST and spa types. One methicillin-resistant S. aureus strain (MRSA) of human origin, ST80-t053 and PFGE type. C is included as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4663800&req=5

Figure 1: Dendogram of PFGE banding patterns of the isolated S. aureus strains and the respective MLST and spa types. One methicillin-resistant S. aureus strain (MRSA) of human origin, ST80-t053 and PFGE type. C is included as control.

Mentions: All isolates were tested by PCR for the presence of mecA, mecC (Stegger et al., 2012), the genes encoding Panton-Valentine leukocidin (PVL) (lukF/lukS-PV) and toxic shock syndrome toxin-1 (TSST-1, tst) (Jarraud et al., 2002), using the reference strains ATCC49775, LGA251, ATCC49775 and Fri913 as positive controls, respectively. DNA extraction was performed into agarose disks, whereas pulsed-field gel electrophoresis (PFGE) of SmaI DNA digests was carried out using a CHEF DR III apparatus (Bio-Rad Laboratories, Richmond, CA, USA). Visual interpretation of PFGE banding patterns and assignments of types were performed according to the published criteria (Tenover et al., 1995). PFGE types were named by capital letters and compared to previously identified pulsotypes among humans. A dendrogram was computed comparing molecular weights of strains’ of DNA fragments by using FPQuest software (Bio-Rad, Cat.Num:1709300). Clustering was based on ≥ 80% similarity (Fig. 1). Multi Locus Sequence Typing (MLST) was performed according to published protocols (http://saureus.mlst.net/misc/info.asp) (Enright et al., 2000). The polymorphic X-region of protein A gene (spa) was analyzed (Stegger et al., 2012) and the resulting spa types and clonal clusters were assigned using the software Ridom StaphType (Ridom GmbH, Wurzburg, Germany).


Human Staphylococcus aureus lineages among Zoological Park residents in Greece.

Drougka E, Foka A, Posantzis D, Giormezis N, Anastassiou ED, Petinaki E, Spiliopoulou I - Open Vet J (2015)

Dendogram of PFGE banding patterns of the isolated S. aureus strains and the respective MLST and spa types. One methicillin-resistant S. aureus strain (MRSA) of human origin, ST80-t053 and PFGE type. C is included as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663800&req=5

Figure 1: Dendogram of PFGE banding patterns of the isolated S. aureus strains and the respective MLST and spa types. One methicillin-resistant S. aureus strain (MRSA) of human origin, ST80-t053 and PFGE type. C is included as control.
Mentions: All isolates were tested by PCR for the presence of mecA, mecC (Stegger et al., 2012), the genes encoding Panton-Valentine leukocidin (PVL) (lukF/lukS-PV) and toxic shock syndrome toxin-1 (TSST-1, tst) (Jarraud et al., 2002), using the reference strains ATCC49775, LGA251, ATCC49775 and Fri913 as positive controls, respectively. DNA extraction was performed into agarose disks, whereas pulsed-field gel electrophoresis (PFGE) of SmaI DNA digests was carried out using a CHEF DR III apparatus (Bio-Rad Laboratories, Richmond, CA, USA). Visual interpretation of PFGE banding patterns and assignments of types were performed according to the published criteria (Tenover et al., 1995). PFGE types were named by capital letters and compared to previously identified pulsotypes among humans. A dendrogram was computed comparing molecular weights of strains’ of DNA fragments by using FPQuest software (Bio-Rad, Cat.Num:1709300). Clustering was based on ≥ 80% similarity (Fig. 1). Multi Locus Sequence Typing (MLST) was performed according to published protocols (http://saureus.mlst.net/misc/info.asp) (Enright et al., 2000). The polymorphic X-region of protein A gene (spa) was analyzed (Stegger et al., 2012) and the resulting spa types and clonal clusters were assigned using the software Ridom StaphType (Ridom GmbH, Wurzburg, Germany).

Bottom Line: Clones were defined by Multilocus Sequence Typing (MLST), spa type and Pulsed-Field Gel Electrophoresis (PFGE).Seven S. aureus isolates were recovered from four animals and one from an employee.Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, University of Patras, Patras, Greece ; National Reference Laboratory for Staphylococci, University of Patras, Patras, Greece.

ABSTRACT
Staphylococcus aureus is a part of the microbiota flora in many animal species. The clonal spread of S. aureus among animals and personnel in a Zoological Park was investigated. Samples were collected from colonized and infected sites among 32 mammals, 11 birds and eight humans. The genes mecA, mecC, lukF/lukS-PV (encoding Panton-Valentine leukocidin, PVL) and tst (toxic shock syndrome toxin-1) were investigated by PCR. Clones were defined by Multilocus Sequence Typing (MLST), spa type and Pulsed-Field Gel Electrophoresis (PFGE). Seven S. aureus isolates were recovered from four animals and one from an employee. All were mecA, mecC and tst-negative, whereas, one carried the PVL genes and was isolated from an infected Squirrel monkey. Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin. Even though a variety of genotypes were identified among S. aureus strains colonizing zoo park residents, our results indicate that colonization with human lineages has indeed occurred.

No MeSH data available.


Related in: MedlinePlus