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In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.

Manivannan A, Soundararajan P, Park YG, Jeong BR - Biomed Res Int (2015)

Bottom Line: The regenerated plants displayed successful survival ratio (95%) in the greenhouse.The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts.Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

No MeSH data available.


Related in: MedlinePlus

Free radical scavenging potentials of in vitro and in vivo extracts. (a–d) Nitric oxide scavenging, superoxide scavenging, hydrogen peroxide scavenging, and DPPH radical scavenging activities of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis and ascorbic acid (AA). Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.
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fig4: Free radical scavenging potentials of in vitro and in vivo extracts. (a–d) Nitric oxide scavenging, superoxide scavenging, hydrogen peroxide scavenging, and DPPH radical scavenging activities of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis and ascorbic acid (AA). Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.

Mentions: The results of NO scavenging assay revealed the substantial ability of the extracts to scavenge NO radical (Figure 4(a)). ISE scavenged 83.49% of NO radicals followed by SE (82.60%). Moreover, the CE exhibited 71.74% of NO inhibition followed by root extracts, respectively. In biological systems, the excess generation of NO radicals leads to various ailments such as chronic inflammatory disorders, carcinogenesis, and atherosclerosis [37]. Therefore, it is highly important to determine the ability of the extracts to inhibit the NO radicals to prevent dreadful diseases.


In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.

Manivannan A, Soundararajan P, Park YG, Jeong BR - Biomed Res Int (2015)

Free radical scavenging potentials of in vitro and in vivo extracts. (a–d) Nitric oxide scavenging, superoxide scavenging, hydrogen peroxide scavenging, and DPPH radical scavenging activities of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis and ascorbic acid (AA). Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663745&req=5

fig4: Free radical scavenging potentials of in vitro and in vivo extracts. (a–d) Nitric oxide scavenging, superoxide scavenging, hydrogen peroxide scavenging, and DPPH radical scavenging activities of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis and ascorbic acid (AA). Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.
Mentions: The results of NO scavenging assay revealed the substantial ability of the extracts to scavenge NO radical (Figure 4(a)). ISE scavenged 83.49% of NO radicals followed by SE (82.60%). Moreover, the CE exhibited 71.74% of NO inhibition followed by root extracts, respectively. In biological systems, the excess generation of NO radicals leads to various ailments such as chronic inflammatory disorders, carcinogenesis, and atherosclerosis [37]. Therefore, it is highly important to determine the ability of the extracts to inhibit the NO radicals to prevent dreadful diseases.

Bottom Line: The regenerated plants displayed successful survival ratio (95%) in the greenhouse.The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts.Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

No MeSH data available.


Related in: MedlinePlus