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In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.

Manivannan A, Soundararajan P, Park YG, Jeong BR - Biomed Res Int (2015)

Bottom Line: The regenerated plants displayed successful survival ratio (95%) in the greenhouse.The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts.Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

No MeSH data available.


Related in: MedlinePlus

Phytochemical contents of in vitro and in vivo extracts. (a-b) Total phenol and total flavonoid contents present in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis. Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.
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fig2: Phytochemical contents of in vitro and in vivo extracts. (a-b) Total phenol and total flavonoid contents present in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis. Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.

Mentions: The characterization of phytochemical contents of different tissues of S. kakudensis is crucial to determine its pharmaceutical importance. The total phenol and flavonoid contents of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extract (CE), in vivo shoot extract (SE), and in vivo root extract (RE) were shown in Figure 2. Among the tissues, shoot contained larger amount of total phenol (Figure 2(a)) and flavonoid contents (Figure 2(b)) than root extracts. According to Amoo et al. [29] the aerial parts of plants are often suitable for the synthesis and accumulation of secondary metabolites in abundance compared to the underground parts. Moreover, the results indicated the accumulation levels of total phenols and flavonoids were nonsignificantly different in ISE and SE. Furthermore, IRE and RE displayed nonsignificant increase in phenol content and RE consisted of higher flavonoids than IRE. Moreover, the CE was also noted with the significant amount of total phenol (31.12 μg GAE·mg−1 FW) and flavonoid phytochemical contents (14.88 μg quercetin·mg−1 FW). The variation in the production of bioactive compounds in different tissues can be due to the differences in the tissue dependent synthesis and accumulation of phytochemicals, physiological condition of the plant part, and endogenous hormone levels [30]. This result denoted that the contents of phytochemicals in seed derived and in vitro derived plants were similar and the in vitro plantlets can be considered as the alternate for the seed grown plants in terms of therapeutic values.


In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.

Manivannan A, Soundararajan P, Park YG, Jeong BR - Biomed Res Int (2015)

Phytochemical contents of in vitro and in vivo extracts. (a-b) Total phenol and total flavonoid contents present in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis. Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663745&req=5

fig2: Phytochemical contents of in vitro and in vivo extracts. (a-b) Total phenol and total flavonoid contents present in vitro shoot extract (ISE), in vitro root extract (IRE), callus extracts (CEs), in vivo shoot extract (SE), and in vivo root extract (RE) of S. kakudensis. Different letters in one measurement indicate statistically significant difference at P ≤ 0.05 by Duncan multiple range test.
Mentions: The characterization of phytochemical contents of different tissues of S. kakudensis is crucial to determine its pharmaceutical importance. The total phenol and flavonoid contents of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extract (CE), in vivo shoot extract (SE), and in vivo root extract (RE) were shown in Figure 2. Among the tissues, shoot contained larger amount of total phenol (Figure 2(a)) and flavonoid contents (Figure 2(b)) than root extracts. According to Amoo et al. [29] the aerial parts of plants are often suitable for the synthesis and accumulation of secondary metabolites in abundance compared to the underground parts. Moreover, the results indicated the accumulation levels of total phenols and flavonoids were nonsignificantly different in ISE and SE. Furthermore, IRE and RE displayed nonsignificant increase in phenol content and RE consisted of higher flavonoids than IRE. Moreover, the CE was also noted with the significant amount of total phenol (31.12 μg GAE·mg−1 FW) and flavonoid phytochemical contents (14.88 μg quercetin·mg−1 FW). The variation in the production of bioactive compounds in different tissues can be due to the differences in the tissue dependent synthesis and accumulation of phytochemicals, physiological condition of the plant part, and endogenous hormone levels [30]. This result denoted that the contents of phytochemicals in seed derived and in vitro derived plants were similar and the in vitro plantlets can be considered as the alternate for the seed grown plants in terms of therapeutic values.

Bottom Line: The regenerated plants displayed successful survival ratio (95%) in the greenhouse.The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts.Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

No MeSH data available.


Related in: MedlinePlus