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In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.

Manivannan A, Soundararajan P, Park YG, Jeong BR - Biomed Res Int (2015)

Bottom Line: The regenerated plants displayed successful survival ratio (95%) in the greenhouse.The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts.Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

No MeSH data available.


Related in: MedlinePlus

Adventitious shoot organogenesis in S. kakudensis Franch. (a) Clump of green colored protuberances appeared from the nodal explant. (b) Early stage of adventitious shoot induction with small leaves indicated by an arrow. (c) Adventitious shoots induced after three weeks. (d) Shoot elongation. (e) In vitro root induction from the shoot base. (f) Acclimatized plants in a greenhouse.
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fig1: Adventitious shoot organogenesis in S. kakudensis Franch. (a) Clump of green colored protuberances appeared from the nodal explant. (b) Early stage of adventitious shoot induction with small leaves indicated by an arrow. (c) Adventitious shoots induced after three weeks. (d) Shoot elongation. (e) In vitro root induction from the shoot base. (f) Acclimatized plants in a greenhouse.

Mentions: In the present investigation, adventitious shoot organogenesis was established from nodal explants derived from the axenic in vitro grown shoots by different concentrations and combinations of PGRs. Initially, the seeds were germinated in vitro and the seedlings were grown in PGR-free MS medium. After three subcultures in four-week interval, the nodal explants were excised and inoculated on the hormone-free MS medium (control), MS medium containing different concentrations, and combinations of PGRs for shoot multiplication. Explants cultured on the PGR-free MS medium produced only a single shoot per explant. Similarly, the explant cultured on shoot induction medium supplemented with three different cytokinins produced several adventitious shoots. Initially, the nodal explants cultured on the cytokinins-fortified medium were bloated within 10–12 days and the adventitious shoots appeared as small multiple protuberances on the cut surfaces (Figure 1(a)). After two weeks, caulogenesis was identified with small leaf-like structures observed from the outgrowths which subsequently developed into adventitious shoots (Figure 1(b)).


In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.

Manivannan A, Soundararajan P, Park YG, Jeong BR - Biomed Res Int (2015)

Adventitious shoot organogenesis in S. kakudensis Franch. (a) Clump of green colored protuberances appeared from the nodal explant. (b) Early stage of adventitious shoot induction with small leaves indicated by an arrow. (c) Adventitious shoots induced after three weeks. (d) Shoot elongation. (e) In vitro root induction from the shoot base. (f) Acclimatized plants in a greenhouse.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663745&req=5

fig1: Adventitious shoot organogenesis in S. kakudensis Franch. (a) Clump of green colored protuberances appeared from the nodal explant. (b) Early stage of adventitious shoot induction with small leaves indicated by an arrow. (c) Adventitious shoots induced after three weeks. (d) Shoot elongation. (e) In vitro root induction from the shoot base. (f) Acclimatized plants in a greenhouse.
Mentions: In the present investigation, adventitious shoot organogenesis was established from nodal explants derived from the axenic in vitro grown shoots by different concentrations and combinations of PGRs. Initially, the seeds were germinated in vitro and the seedlings were grown in PGR-free MS medium. After three subcultures in four-week interval, the nodal explants were excised and inoculated on the hormone-free MS medium (control), MS medium containing different concentrations, and combinations of PGRs for shoot multiplication. Explants cultured on the PGR-free MS medium produced only a single shoot per explant. Similarly, the explant cultured on shoot induction medium supplemented with three different cytokinins produced several adventitious shoots. Initially, the nodal explants cultured on the cytokinins-fortified medium were bloated within 10–12 days and the adventitious shoots appeared as small multiple protuberances on the cut surfaces (Figure 1(a)). After two weeks, caulogenesis was identified with small leaf-like structures observed from the outgrowths which subsequently developed into adventitious shoots (Figure 1(b)).

Bottom Line: The regenerated plants displayed successful survival ratio (95%) in the greenhouse.The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts.Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.

No MeSH data available.


Related in: MedlinePlus