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Surface Deposition and Coalescence and Coacervation Phase Separation Methods: In Vitro Study and Compatibility Analysis of Eudragit RS30D, Eudragit RL30D, and Carbopol-PLA Loaded Metronidazole Microspheres.

Dewan I, Islam MM, Al-Hasan M, Nath J, Sultana S, Rana MS - J Pharm (Cairo) (2015)

Bottom Line: Metronidazole (MTZ) has extremely broad spectrum of protozoal and antimicrobial activity and is clinically effective in trichomoniasis, amoebic colitis, and giardiasis.The SEM and FTIR studies confirm good spheres and smooth surface as well as interaction between drug and polymers.Though release kinetic is uncertain, the best fit was obtained with the Korsmeyer kinetic model with release exponent (n) lying between 0.45 and 0.89.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka 1209, Bangladesh.

ABSTRACT
Metronidazole (MTZ) has extremely broad spectrum of protozoal and antimicrobial activity and is clinically effective in trichomoniasis, amoebic colitis, and giardiasis. This study was performed to formulate and evaluate the MTZ loaded microspheres by coacervation phase separation and surface deposition and coalescence methods using different polymers like Gelatin, Carbopol 934P, Polylactic Acid (PLA), Eudragit RS30D, and Eudragit RL30D to acquire sustained release of drug. In vitro dissolution studies were carried out in phosphate buffer (pH 7.4) for 8 hours according to USP paddle method. The maximum and minimum release of MTZ from microspheres observed were 84.81% and 76.6% for coacervation and 95.07% and 80.07% for surface deposition method, respectively, after 8 hours. Release kinetics was studied in different mathematical release models. The SEM and FTIR studies confirm good spheres and smooth surface as well as interaction between drug and polymers. Though release kinetic is uncertain, the best fit was obtained with the Korsmeyer kinetic model with release exponent (n) lying between 0.45 and 0.89. In vitro studies showed that MTZ microspheres with different polymers might be a good candidate as sustained drug delivery system to treat bacterial infections.

No MeSH data available.


Related in: MedlinePlus

FTIR spectrum of (a) pure drug metronidazole, (b) formulation F26, (c) formulation F28, Eudragit RS30D, and Eudragit RL30D, respectively. %  T: % transmittance or absorbance.
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fig7: FTIR spectrum of (a) pure drug metronidazole, (b) formulation F26, (c) formulation F28, Eudragit RS30D, and Eudragit RL30D, respectively. %  T: % transmittance or absorbance.

Mentions: The FTIR technique is to measure the absorption of various infrared radiations by the target material and to produce an IR spectrum that can be used to identify functional groups and molecular structure in the sample shown in Figure 7. FTIR spectra of pure MTZ and formulated microspheres were recorded by using FTIR 8400S (SHIMADZU, Japan). Appropriate quantities of KBr and microspheres (in the ratio 100 : 2) were mixed by grinding in an agate mortar. Disk was made with about 100 mg mixture under hydraulic pressure of 600 kg. Then the FTIR spectra were recorded between 4000 and 400 cm−1. The resolution was 2 cm−1.


Surface Deposition and Coalescence and Coacervation Phase Separation Methods: In Vitro Study and Compatibility Analysis of Eudragit RS30D, Eudragit RL30D, and Carbopol-PLA Loaded Metronidazole Microspheres.

Dewan I, Islam MM, Al-Hasan M, Nath J, Sultana S, Rana MS - J Pharm (Cairo) (2015)

FTIR spectrum of (a) pure drug metronidazole, (b) formulation F26, (c) formulation F28, Eudragit RS30D, and Eudragit RL30D, respectively. %  T: % transmittance or absorbance.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663742&req=5

fig7: FTIR spectrum of (a) pure drug metronidazole, (b) formulation F26, (c) formulation F28, Eudragit RS30D, and Eudragit RL30D, respectively. %  T: % transmittance or absorbance.
Mentions: The FTIR technique is to measure the absorption of various infrared radiations by the target material and to produce an IR spectrum that can be used to identify functional groups and molecular structure in the sample shown in Figure 7. FTIR spectra of pure MTZ and formulated microspheres were recorded by using FTIR 8400S (SHIMADZU, Japan). Appropriate quantities of KBr and microspheres (in the ratio 100 : 2) were mixed by grinding in an agate mortar. Disk was made with about 100 mg mixture under hydraulic pressure of 600 kg. Then the FTIR spectra were recorded between 4000 and 400 cm−1. The resolution was 2 cm−1.

Bottom Line: Metronidazole (MTZ) has extremely broad spectrum of protozoal and antimicrobial activity and is clinically effective in trichomoniasis, amoebic colitis, and giardiasis.The SEM and FTIR studies confirm good spheres and smooth surface as well as interaction between drug and polymers.Though release kinetic is uncertain, the best fit was obtained with the Korsmeyer kinetic model with release exponent (n) lying between 0.45 and 0.89.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka 1209, Bangladesh.

ABSTRACT
Metronidazole (MTZ) has extremely broad spectrum of protozoal and antimicrobial activity and is clinically effective in trichomoniasis, amoebic colitis, and giardiasis. This study was performed to formulate and evaluate the MTZ loaded microspheres by coacervation phase separation and surface deposition and coalescence methods using different polymers like Gelatin, Carbopol 934P, Polylactic Acid (PLA), Eudragit RS30D, and Eudragit RL30D to acquire sustained release of drug. In vitro dissolution studies were carried out in phosphate buffer (pH 7.4) for 8 hours according to USP paddle method. The maximum and minimum release of MTZ from microspheres observed were 84.81% and 76.6% for coacervation and 95.07% and 80.07% for surface deposition method, respectively, after 8 hours. Release kinetics was studied in different mathematical release models. The SEM and FTIR studies confirm good spheres and smooth surface as well as interaction between drug and polymers. Though release kinetic is uncertain, the best fit was obtained with the Korsmeyer kinetic model with release exponent (n) lying between 0.45 and 0.89. In vitro studies showed that MTZ microspheres with different polymers might be a good candidate as sustained drug delivery system to treat bacterial infections.

No MeSH data available.


Related in: MedlinePlus