Limits...
Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7.

Jorjão AL, de Oliveira FE, Leão MV, Carvalho CA, Jorge AO, de Oliveira LD - ScientificWorldJournal (2015)

Bottom Line: The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%.All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0.05).In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, 777 Jardim São Dimas, 12245-000 São José dos Campos, SP, Brazil.

ABSTRACT
This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0.05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0.05) but not IL-6 (P > 0.05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0.05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.

No MeSH data available.


Related in: MedlinePlus

Cytokines secreted by macrophages after exposure to different suspensions: LPS (positive control); negative control (saline solution); LLR: live L. rhamnosus; HKLR: heat-killed L. rhamnosus; and SHKLR: supernatant of heat-killed L. rhamnosus. Mean values (pg/mL) ± standard deviation of (a) TNF-α, (b) IL-6, (c) IL-1β, (d) IL-12, (e) IL-10, and (f) IL-4.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4663741&req=5

fig1: Cytokines secreted by macrophages after exposure to different suspensions: LPS (positive control); negative control (saline solution); LLR: live L. rhamnosus; HKLR: heat-killed L. rhamnosus; and SHKLR: supernatant of heat-killed L. rhamnosus. Mean values (pg/mL) ± standard deviation of (a) TNF-α, (b) IL-6, (c) IL-1β, (d) IL-12, (e) IL-10, and (f) IL-4.

Mentions: The suspensions containing live L. rhamnosus (LLR) or heat-killed L. rhamnosus (HKLR) were able to induce significant production of TNF-α in the same amounts as LPS (P > 0.05). The suspensions with only the products of the microorganism (SHKLR) also significantly induced the production of this cytokine when compared with the negative control, although at a lower level than the other groups (LLR, HKLR, and LPS) (P < 0.05) (Figure 1(a)).


Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7.

Jorjão AL, de Oliveira FE, Leão MV, Carvalho CA, Jorge AO, de Oliveira LD - ScientificWorldJournal (2015)

Cytokines secreted by macrophages after exposure to different suspensions: LPS (positive control); negative control (saline solution); LLR: live L. rhamnosus; HKLR: heat-killed L. rhamnosus; and SHKLR: supernatant of heat-killed L. rhamnosus. Mean values (pg/mL) ± standard deviation of (a) TNF-α, (b) IL-6, (c) IL-1β, (d) IL-12, (e) IL-10, and (f) IL-4.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663741&req=5

fig1: Cytokines secreted by macrophages after exposure to different suspensions: LPS (positive control); negative control (saline solution); LLR: live L. rhamnosus; HKLR: heat-killed L. rhamnosus; and SHKLR: supernatant of heat-killed L. rhamnosus. Mean values (pg/mL) ± standard deviation of (a) TNF-α, (b) IL-6, (c) IL-1β, (d) IL-12, (e) IL-10, and (f) IL-4.
Mentions: The suspensions containing live L. rhamnosus (LLR) or heat-killed L. rhamnosus (HKLR) were able to induce significant production of TNF-α in the same amounts as LPS (P > 0.05). The suspensions with only the products of the microorganism (SHKLR) also significantly induced the production of this cytokine when compared with the negative control, although at a lower level than the other groups (LLR, HKLR, and LPS) (P < 0.05) (Figure 1(a)).

Bottom Line: The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%.All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0.05).In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, 777 Jardim São Dimas, 12245-000 São José dos Campos, SP, Brazil.

ABSTRACT
This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0.05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0.05) but not IL-6 (P > 0.05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0.05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.

No MeSH data available.


Related in: MedlinePlus