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Heme oxygenase effect on mesenchymal stem cells action on experimental Alzheimer's disease.

Abdel Aziza MT, Atta HM, Samer H, Ahmed HH, Rashed LA, Sabry D, Abdel Raouf ER, Alkaffas MA - EXCLI J (2013)

Bottom Line: MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain.Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level.MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT
The objective is to evaluate the effect of heme oxygenase-1 (HO-1) enzyme inducer and inhibitor on Mesenchymal Stem Cells (MSCs) in Alzheimer disease. 70 female albino rats were divided equally into 7 groups as follows: group 1: healthy control; group 2: Aluminium chloride induced Alzheimer disease; group 3: induced Alzheimer rats that received intravenous injection of MSCs; group 4: induced Alzheimer rats that received MSCs and HO inducer cobalt protoporphyrin; group 5: induced Alzheimer rats that received MSCs and HO inhibitor zinc protoporphyrin; group 6: induced Alzheimer rats that received HO inducer; group7: induced Alzheimer rats that received HO inhibitor. Brain tissue was collected for HO-1, seladin-1 gene expression by real time polymerase chain reaction, heme oxygenase activity, cholesterol estimation and histopathological examination. MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain. Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level. MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

No MeSH data available.


Related in: MedlinePlus

A) Box plots analysis of heme oxygenase-1, B) Box plots analysis of seladin-1 gene expression by real time PCR in different groups
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Figure 5: A) Box plots analysis of heme oxygenase-1, B) Box plots analysis of seladin-1 gene expression by real time PCR in different groups

Mentions: Concerning gene expression, there was a significant increase in the heme oxygenase-1expression and decrease in the seladin-1 gene expression in the AD group compared to the control group (P<0.05). Following MSC injection, the HO-1 expression was insignificantly increased (P=1.000), while seladin-1 expression increased significantly (P<0.05) compared to the AD group. Following MSC injection with HO inducer, the HO-1 expression increased significantly (P<0.05) compared to the AD group and AD with MSCs, the Seladin-1 expression increased significantly (P<0.05) compared to the AD group but insignificantly compared to AD with MSCs group (P=1.000). Following MSC injection with HO inhibitor, H0-1 expression decreased significantly (P<0.05) compared to the AD group, AD with MSCs and AD with MSCs with HO inducer group while the seladin-1 expression increased significantly (P<0.05) compared to the AD group, AD with MSCs group and AD with MSCs with HO inducer group. Following HO inducer, the HO-1 expression increased significantly (P<0.05) compared to all other groups while the seladin-1 expression decreased significantly (P<0.05) compared to the AD with MSC, AD with MSCs with HO inducer group, AD with MSCs with HO inhibitor group but was insignificantly decreased compared to the AD group (p=0.923). Following HO inhibitor, the HO-1 expression decreased significantly (P<0.05) compared to all other groups while the seladin-1 expression increased significantly (P<0.05) compared to the AD with MSCs, AD with MSCs with HO inducer and the AD with MSC with HO inhibitor group, but was insignificantly decreased compared to AD group (p=0.949) and the AD with HO inducer group (P=0.872 (Figure 5A, B(Fig. 5))).


Heme oxygenase effect on mesenchymal stem cells action on experimental Alzheimer's disease.

Abdel Aziza MT, Atta HM, Samer H, Ahmed HH, Rashed LA, Sabry D, Abdel Raouf ER, Alkaffas MA - EXCLI J (2013)

A) Box plots analysis of heme oxygenase-1, B) Box plots analysis of seladin-1 gene expression by real time PCR in different groups
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663655&req=5

Figure 5: A) Box plots analysis of heme oxygenase-1, B) Box plots analysis of seladin-1 gene expression by real time PCR in different groups
Mentions: Concerning gene expression, there was a significant increase in the heme oxygenase-1expression and decrease in the seladin-1 gene expression in the AD group compared to the control group (P<0.05). Following MSC injection, the HO-1 expression was insignificantly increased (P=1.000), while seladin-1 expression increased significantly (P<0.05) compared to the AD group. Following MSC injection with HO inducer, the HO-1 expression increased significantly (P<0.05) compared to the AD group and AD with MSCs, the Seladin-1 expression increased significantly (P<0.05) compared to the AD group but insignificantly compared to AD with MSCs group (P=1.000). Following MSC injection with HO inhibitor, H0-1 expression decreased significantly (P<0.05) compared to the AD group, AD with MSCs and AD with MSCs with HO inducer group while the seladin-1 expression increased significantly (P<0.05) compared to the AD group, AD with MSCs group and AD with MSCs with HO inducer group. Following HO inducer, the HO-1 expression increased significantly (P<0.05) compared to all other groups while the seladin-1 expression decreased significantly (P<0.05) compared to the AD with MSC, AD with MSCs with HO inducer group, AD with MSCs with HO inhibitor group but was insignificantly decreased compared to the AD group (p=0.923). Following HO inhibitor, the HO-1 expression decreased significantly (P<0.05) compared to all other groups while the seladin-1 expression increased significantly (P<0.05) compared to the AD with MSCs, AD with MSCs with HO inducer and the AD with MSC with HO inhibitor group, but was insignificantly decreased compared to AD group (p=0.949) and the AD with HO inducer group (P=0.872 (Figure 5A, B(Fig. 5))).

Bottom Line: MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain.Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level.MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT
The objective is to evaluate the effect of heme oxygenase-1 (HO-1) enzyme inducer and inhibitor on Mesenchymal Stem Cells (MSCs) in Alzheimer disease. 70 female albino rats were divided equally into 7 groups as follows: group 1: healthy control; group 2: Aluminium chloride induced Alzheimer disease; group 3: induced Alzheimer rats that received intravenous injection of MSCs; group 4: induced Alzheimer rats that received MSCs and HO inducer cobalt protoporphyrin; group 5: induced Alzheimer rats that received MSCs and HO inhibitor zinc protoporphyrin; group 6: induced Alzheimer rats that received HO inducer; group7: induced Alzheimer rats that received HO inhibitor. Brain tissue was collected for HO-1, seladin-1 gene expression by real time polymerase chain reaction, heme oxygenase activity, cholesterol estimation and histopathological examination. MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain. Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level. MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

No MeSH data available.


Related in: MedlinePlus