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Heme oxygenase effect on mesenchymal stem cells action on experimental Alzheimer's disease.

Abdel Aziza MT, Atta HM, Samer H, Ahmed HH, Rashed LA, Sabry D, Abdel Raouf ER, Alkaffas MA - EXCLI J (2013)

Bottom Line: MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain.Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level.MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT
The objective is to evaluate the effect of heme oxygenase-1 (HO-1) enzyme inducer and inhibitor on Mesenchymal Stem Cells (MSCs) in Alzheimer disease. 70 female albino rats were divided equally into 7 groups as follows: group 1: healthy control; group 2: Aluminium chloride induced Alzheimer disease; group 3: induced Alzheimer rats that received intravenous injection of MSCs; group 4: induced Alzheimer rats that received MSCs and HO inducer cobalt protoporphyrin; group 5: induced Alzheimer rats that received MSCs and HO inhibitor zinc protoporphyrin; group 6: induced Alzheimer rats that received HO inducer; group7: induced Alzheimer rats that received HO inhibitor. Brain tissue was collected for HO-1, seladin-1 gene expression by real time polymerase chain reaction, heme oxygenase activity, cholesterol estimation and histopathological examination. MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain. Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level. MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

No MeSH data available.


Related in: MedlinePlus

Morphological and histological staining of BM-MSCs differentiated into chondrocytes(A) (×20) Arrows for differentiated MSCs chondrocytes after addition of growth factors(B) (×200) MSCs differentiated into chondrocytes stained with Alcian blue stain
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Figure 2: Morphological and histological staining of BM-MSCs differentiated into chondrocytes(A) (×20) Arrows for differentiated MSCs chondrocytes after addition of growth factors(B) (×200) MSCs differentiated into chondrocytes stained with Alcian blue stain

Mentions: Isolated and cultured undifferentiated MSCs reached 70-80 % confluence in 14 days. In vitro osteogenic and chondrogenic differentiation of MSCs were confirmed by morphological changes and special stains (Figure 1A, B(Fig. 1) and Figure 2A, B(Fig. 2) respectively). In addition MSCs were identified by surface marker CD29 (+) by PCR (Figure 3(Fig. 3)). MSCs labeled with PKH26 fluorescent dye were detected in the brain tissues confirming that these cells homed into the brain tissues (Figure 4(Fig. 4)).


Heme oxygenase effect on mesenchymal stem cells action on experimental Alzheimer's disease.

Abdel Aziza MT, Atta HM, Samer H, Ahmed HH, Rashed LA, Sabry D, Abdel Raouf ER, Alkaffas MA - EXCLI J (2013)

Morphological and histological staining of BM-MSCs differentiated into chondrocytes(A) (×20) Arrows for differentiated MSCs chondrocytes after addition of growth factors(B) (×200) MSCs differentiated into chondrocytes stained with Alcian blue stain
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663655&req=5

Figure 2: Morphological and histological staining of BM-MSCs differentiated into chondrocytes(A) (×20) Arrows for differentiated MSCs chondrocytes after addition of growth factors(B) (×200) MSCs differentiated into chondrocytes stained with Alcian blue stain
Mentions: Isolated and cultured undifferentiated MSCs reached 70-80 % confluence in 14 days. In vitro osteogenic and chondrogenic differentiation of MSCs were confirmed by morphological changes and special stains (Figure 1A, B(Fig. 1) and Figure 2A, B(Fig. 2) respectively). In addition MSCs were identified by surface marker CD29 (+) by PCR (Figure 3(Fig. 3)). MSCs labeled with PKH26 fluorescent dye were detected in the brain tissues confirming that these cells homed into the brain tissues (Figure 4(Fig. 4)).

Bottom Line: MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain.Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level.MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT
The objective is to evaluate the effect of heme oxygenase-1 (HO-1) enzyme inducer and inhibitor on Mesenchymal Stem Cells (MSCs) in Alzheimer disease. 70 female albino rats were divided equally into 7 groups as follows: group 1: healthy control; group 2: Aluminium chloride induced Alzheimer disease; group 3: induced Alzheimer rats that received intravenous injection of MSCs; group 4: induced Alzheimer rats that received MSCs and HO inducer cobalt protoporphyrin; group 5: induced Alzheimer rats that received MSCs and HO inhibitor zinc protoporphyrin; group 6: induced Alzheimer rats that received HO inducer; group7: induced Alzheimer rats that received HO inhibitor. Brain tissue was collected for HO-1, seladin-1 gene expression by real time polymerase chain reaction, heme oxygenase activity, cholesterol estimation and histopathological examination. MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain. Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level. MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

No MeSH data available.


Related in: MedlinePlus