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Tomato (Lycopersicon esculentum) Supplementation Induces Changes in Cardiac miRNA Expression, Reduces Oxidative Stress and Left Ventricular Mass, and Improves Diastolic Function.

Pereira BL, Arruda FC, Reis PP, Felix TF, Santos PP, Rafacho BP, Gonçalves AF, Claro RT, Azevedo PS, Polegato BF, Okoshi K, Fernandes AA, Paiva SA, Zornoff LA, Minicucci MF - Nutrients (2015)

Bottom Line: Tomato intake was also associated with up-regulation of miR-107 and miR-486 and down-regulation of miR-350 and miR-872.In conclusion, tomato supplementation induces changes in miRNA expression and reduces oxidative stress.In addition, these alterations may be responsible for CSA reduction and diastolic function improvement.

View Article: PubMed Central - PubMed

Affiliation: Internal Medicine Department, Botucatu Medical School, Univ Estadual Paulista (UNESP), Botucatu 18618-970, Brazil. bruh_leticia@hotmail.com.

ABSTRACT
The aim of this study was to evaluate the effects of tomato supplementation on the normal rat heart and the role of oxidative stress in this scenario. Male Wistar rats were assigned to two groups: a control group (C; n = 16), in which animals received a control diet + 0.5 mL of corn oil/kg body weight/day, and a tomato group (T; n = 16), in which animals received a control diet supplemented with tomato +0.5 mL of corn oil/kg body weight/day. After three months, morphological, functional, and biochemical analyses were performed. Animals supplemented with tomato had a smaller left atrium diameter and myocyte cross-sectional area (CSA) compared to the control group (C group: 474 (415-539); T group: 273 (258-297) µm²; p = 0.004). Diastolic function was improved in rats supplemented with tomato. In addition, lipid hydroperoxide was lower (C group: 267 ± 46.7; T group: 219 ± 23.0 nmol/g; p = 0.039) in the myocardium of rats supplemented with tomato. Tomato intake was also associated with up-regulation of miR-107 and miR-486 and down-regulation of miR-350 and miR-872. In conclusion, tomato supplementation induces changes in miRNA expression and reduces oxidative stress. In addition, these alterations may be responsible for CSA reduction and diastolic function improvement.

No MeSH data available.


Related in: MedlinePlus

(A) Dendogram obtained by hierarchical clustering of 11 samples, six from experimental and five from control groups. Tree view shows data visualization representing miRNAs and samples. miRNA cluster shows over-expressed (red) and under-expressed (green) miRNAs. Black color means unchanged; and (B) volcano plot of differentially expressed miRNAs in tomato versus control group.
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nutrients-07-05493-f003: (A) Dendogram obtained by hierarchical clustering of 11 samples, six from experimental and five from control groups. Tree view shows data visualization representing miRNAs and samples. miRNA cluster shows over-expressed (red) and under-expressed (green) miRNAs. Black color means unchanged; and (B) volcano plot of differentially expressed miRNAs in tomato versus control group.

Mentions: Clustering results showed that samples from the tomato group clustered separately from control samples. Regarding LV miRNA expression, tomato intake was significantly associated with the up-regulation of miR-107 (p = 0.043) and miR-486 (p = 0.001) and the down-regulation of miR-350 (p = 0.035) and miR-872 (p = 0.037) (Figure 3).


Tomato (Lycopersicon esculentum) Supplementation Induces Changes in Cardiac miRNA Expression, Reduces Oxidative Stress and Left Ventricular Mass, and Improves Diastolic Function.

Pereira BL, Arruda FC, Reis PP, Felix TF, Santos PP, Rafacho BP, Gonçalves AF, Claro RT, Azevedo PS, Polegato BF, Okoshi K, Fernandes AA, Paiva SA, Zornoff LA, Minicucci MF - Nutrients (2015)

(A) Dendogram obtained by hierarchical clustering of 11 samples, six from experimental and five from control groups. Tree view shows data visualization representing miRNAs and samples. miRNA cluster shows over-expressed (red) and under-expressed (green) miRNAs. Black color means unchanged; and (B) volcano plot of differentially expressed miRNAs in tomato versus control group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663621&req=5

nutrients-07-05493-f003: (A) Dendogram obtained by hierarchical clustering of 11 samples, six from experimental and five from control groups. Tree view shows data visualization representing miRNAs and samples. miRNA cluster shows over-expressed (red) and under-expressed (green) miRNAs. Black color means unchanged; and (B) volcano plot of differentially expressed miRNAs in tomato versus control group.
Mentions: Clustering results showed that samples from the tomato group clustered separately from control samples. Regarding LV miRNA expression, tomato intake was significantly associated with the up-regulation of miR-107 (p = 0.043) and miR-486 (p = 0.001) and the down-regulation of miR-350 (p = 0.035) and miR-872 (p = 0.037) (Figure 3).

Bottom Line: Tomato intake was also associated with up-regulation of miR-107 and miR-486 and down-regulation of miR-350 and miR-872.In conclusion, tomato supplementation induces changes in miRNA expression and reduces oxidative stress.In addition, these alterations may be responsible for CSA reduction and diastolic function improvement.

View Article: PubMed Central - PubMed

Affiliation: Internal Medicine Department, Botucatu Medical School, Univ Estadual Paulista (UNESP), Botucatu 18618-970, Brazil. bruh_leticia@hotmail.com.

ABSTRACT
The aim of this study was to evaluate the effects of tomato supplementation on the normal rat heart and the role of oxidative stress in this scenario. Male Wistar rats were assigned to two groups: a control group (C; n = 16), in which animals received a control diet + 0.5 mL of corn oil/kg body weight/day, and a tomato group (T; n = 16), in which animals received a control diet supplemented with tomato +0.5 mL of corn oil/kg body weight/day. After three months, morphological, functional, and biochemical analyses were performed. Animals supplemented with tomato had a smaller left atrium diameter and myocyte cross-sectional area (CSA) compared to the control group (C group: 474 (415-539); T group: 273 (258-297) µm²; p = 0.004). Diastolic function was improved in rats supplemented with tomato. In addition, lipid hydroperoxide was lower (C group: 267 ± 46.7; T group: 219 ± 23.0 nmol/g; p = 0.039) in the myocardium of rats supplemented with tomato. Tomato intake was also associated with up-regulation of miR-107 and miR-486 and down-regulation of miR-350 and miR-872. In conclusion, tomato supplementation induces changes in miRNA expression and reduces oxidative stress. In addition, these alterations may be responsible for CSA reduction and diastolic function improvement.

No MeSH data available.


Related in: MedlinePlus