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Rice and Bean Targets for Biofortification Combined with High Carotenoid Content Crops Regulate Transcriptional Mechanisms Increasing Iron Bioavailability.

Dias DM, de Castro Moreira ME, Gomes MJ, Lopes Toledo RC, Nutti MR, Pinheiro Sant'Ana HM, Martino HS - Nutrients (2015)

Bottom Line: Iron bioavailability was measured by the depletion/repletion method.The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency (HRE), gene expression of divalente metal transporter 1 (DMT-1), duodenal citocromo B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC).The PB group presented lower (p < 0.05) TAC than the other groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-000, Minas Gerais, Brazil. desirremorais@hotmail.com.

ABSTRACT
Iron deficiency affects thousands of people worldwide. Biofortification of staple food crops aims to support the reduction of this deficiency. This study evaluates the effect of combinations of common beans and rice, targets for biofortification, with high carotenoid content crops on the iron bioavailability, protein gene expression, and antioxidant effect. Iron bioavailability was measured by the depletion/repletion method. Seven groups were tested (n = 7): Pontal bean (PB); rice + Pontal bean (R + BP); Pontal bean + sweet potato (PB + SP); Pontal bean + pumpkin (PB + P); Pontal bean + rice + sweet potato (PB + R + P); Pontal bean + rice + sweet potato (PB + R + SP); positive control (Ferrous Sulfate). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency (HRE), gene expression of divalente metal transporter 1 (DMT-1), duodenal citocromo B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The test groups, except the PB, showed higher HRE (p < 0.05) than the control. Gene expression of DMT-1, DcytB and ferroportin increased (p < 0.05) in the groups fed with high content carotenoid crops (sweet potato or pumpkin). The PB group presented lower (p < 0.05) TAC than the other groups. The combination of rice and common beans, and those with high carotenoid content crops increased protein gene expression, increasing the iron bioavailability and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus

Effect of the ingestion of different combinations of staple food crops from micronutrients biofortification program and ferrous sulfate on the gene expression of proteins in duodenal tissue. RT-PCR Analysis. (A) Ferroportin; (B) Hephaestin; (C) DMT-1; (D) Dcytb. FS: Sulfate Ferrous; PB: Pontal Bean; PB + R: Pontal Bean + Rice; PB + P: Pontal Bean + Pumpkin; PB + SP: Pontal Bean + Sweet Potato; PB + R + P: Pontal Bean + Rice + Pumpkin; PB + R + SP: Pontal Bean + Rice + Sweet Potato. Different letters indicate statistical differences at 5% probability by Duncan test.
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nutrients-07-05488-f002: Effect of the ingestion of different combinations of staple food crops from micronutrients biofortification program and ferrous sulfate on the gene expression of proteins in duodenal tissue. RT-PCR Analysis. (A) Ferroportin; (B) Hephaestin; (C) DMT-1; (D) Dcytb. FS: Sulfate Ferrous; PB: Pontal Bean; PB + R: Pontal Bean + Rice; PB + P: Pontal Bean + Pumpkin; PB + SP: Pontal Bean + Sweet Potato; PB + R + P: Pontal Bean + Rice + Pumpkin; PB + R + SP: Pontal Bean + Rice + Sweet Potato. Different letters indicate statistical differences at 5% probability by Duncan test.

Mentions: The animals fed diets containing only Pontal bean had lower gene expression of proteins involved in iron metabolism, which may explain the lower HRE (p < 0.05). Additionally, groups containing Pontal bean and high pro-vitamin A carotenoids crops (PB and PB + R + SP) had increased mRNA expression of DcytB (Figure 2D) and ferroportin (Figure 2A).


Rice and Bean Targets for Biofortification Combined with High Carotenoid Content Crops Regulate Transcriptional Mechanisms Increasing Iron Bioavailability.

Dias DM, de Castro Moreira ME, Gomes MJ, Lopes Toledo RC, Nutti MR, Pinheiro Sant'Ana HM, Martino HS - Nutrients (2015)

Effect of the ingestion of different combinations of staple food crops from micronutrients biofortification program and ferrous sulfate on the gene expression of proteins in duodenal tissue. RT-PCR Analysis. (A) Ferroportin; (B) Hephaestin; (C) DMT-1; (D) Dcytb. FS: Sulfate Ferrous; PB: Pontal Bean; PB + R: Pontal Bean + Rice; PB + P: Pontal Bean + Pumpkin; PB + SP: Pontal Bean + Sweet Potato; PB + R + P: Pontal Bean + Rice + Pumpkin; PB + R + SP: Pontal Bean + Rice + Sweet Potato. Different letters indicate statistical differences at 5% probability by Duncan test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663616&req=5

nutrients-07-05488-f002: Effect of the ingestion of different combinations of staple food crops from micronutrients biofortification program and ferrous sulfate on the gene expression of proteins in duodenal tissue. RT-PCR Analysis. (A) Ferroportin; (B) Hephaestin; (C) DMT-1; (D) Dcytb. FS: Sulfate Ferrous; PB: Pontal Bean; PB + R: Pontal Bean + Rice; PB + P: Pontal Bean + Pumpkin; PB + SP: Pontal Bean + Sweet Potato; PB + R + P: Pontal Bean + Rice + Pumpkin; PB + R + SP: Pontal Bean + Rice + Sweet Potato. Different letters indicate statistical differences at 5% probability by Duncan test.
Mentions: The animals fed diets containing only Pontal bean had lower gene expression of proteins involved in iron metabolism, which may explain the lower HRE (p < 0.05). Additionally, groups containing Pontal bean and high pro-vitamin A carotenoids crops (PB and PB + R + SP) had increased mRNA expression of DcytB (Figure 2D) and ferroportin (Figure 2A).

Bottom Line: Iron bioavailability was measured by the depletion/repletion method.The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency (HRE), gene expression of divalente metal transporter 1 (DMT-1), duodenal citocromo B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC).The PB group presented lower (p < 0.05) TAC than the other groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-000, Minas Gerais, Brazil. desirremorais@hotmail.com.

ABSTRACT
Iron deficiency affects thousands of people worldwide. Biofortification of staple food crops aims to support the reduction of this deficiency. This study evaluates the effect of combinations of common beans and rice, targets for biofortification, with high carotenoid content crops on the iron bioavailability, protein gene expression, and antioxidant effect. Iron bioavailability was measured by the depletion/repletion method. Seven groups were tested (n = 7): Pontal bean (PB); rice + Pontal bean (R + BP); Pontal bean + sweet potato (PB + SP); Pontal bean + pumpkin (PB + P); Pontal bean + rice + sweet potato (PB + R + P); Pontal bean + rice + sweet potato (PB + R + SP); positive control (Ferrous Sulfate). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency (HRE), gene expression of divalente metal transporter 1 (DMT-1), duodenal citocromo B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The test groups, except the PB, showed higher HRE (p < 0.05) than the control. Gene expression of DMT-1, DcytB and ferroportin increased (p < 0.05) in the groups fed with high content carotenoid crops (sweet potato or pumpkin). The PB group presented lower (p < 0.05) TAC than the other groups. The combination of rice and common beans, and those with high carotenoid content crops increased protein gene expression, increasing the iron bioavailability and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus