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Fat Quality Influences the Obesogenic Effect of High Fat Diets.

Crescenzo R, Bianco F, Mazzoli A, Giacco A, Cancelliere R, di Fabio G, Zarrelli A, Liverini G, Iossa S - Nutrients (2015)

Bottom Line: Hepatic functionality, plasma parameters, and oxidative status were also measured.The results show that feeding on safflower/linseed oil diet attenuates the obesogenic effect of high fat diets and ameliorates the blood lipid profile.Conversely, hepatic steatosis and mitochondrial oxidative stress appear to be negatively affected by a diet rich in unsaturated fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Federico II University, Via Cinthia, 80138 Naples, Italy. rcrescen@unina.it.

ABSTRACT
High fat and/or carbohydrate intake are associated with an elevated risk for obesity and chronic diseases such as diabetes and cardiovascular diseases. The harmful effects of a high fat diet could be different, depending on dietary fat quality. In fact, high fat diets rich in unsaturated fatty acids are considered less deleterious for human health than those rich in saturated fat. In our previous studies, we have shown that rats fed a high fat diet developed obesity and exhibited a decrease in oxidative capacity and an increase in oxidative stress in liver mitochondria. To investigate whether polyunsaturated fats could attenuate the above deleterious effects of high fat diets, energy balance and body composition were assessed after two weeks in rats fed isocaloric amounts of a high-fat diet (58.2% by energy) rich either in lard or safflower/linseed oil. Hepatic functionality, plasma parameters, and oxidative status were also measured. The results show that feeding on safflower/linseed oil diet attenuates the obesogenic effect of high fat diets and ameliorates the blood lipid profile. Conversely, hepatic steatosis and mitochondrial oxidative stress appear to be negatively affected by a diet rich in unsaturated fatty acids.

No MeSH data available.


Related in: MedlinePlus

Basal (A) and fatty acid-induced (B) proton leak in rats fed lard or safflower-linseed high fat diet. Values are reported as means with their standard errors. n = 8 different rats. Non-linear regression analysis show that curves for rats fed lard were significantly different than those of rats fed safflower-linseed (p < 0.05). In addition, at the same membrane potential (180 mV for basal leak) the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 37.5 ± 2.1; safflower-linseed = 10.5 ± 0.9 ngatoms oxygen/min × mg protein). Similarly, at the same membrane potential of 150 mV for fatty acid-induced proton leak, the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 41.9 ± 2.2; safflower-linseed = 22.2 ± 1.1 ngatoms oxygen/min × mg protein).
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nutrients-07-05480-f004: Basal (A) and fatty acid-induced (B) proton leak in rats fed lard or safflower-linseed high fat diet. Values are reported as means with their standard errors. n = 8 different rats. Non-linear regression analysis show that curves for rats fed lard were significantly different than those of rats fed safflower-linseed (p < 0.05). In addition, at the same membrane potential (180 mV for basal leak) the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 37.5 ± 2.1; safflower-linseed = 10.5 ± 0.9 ngatoms oxygen/min × mg protein). Similarly, at the same membrane potential of 150 mV for fatty acid-induced proton leak, the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 41.9 ± 2.2; safflower-linseed = 22.2 ± 1.1 ngatoms oxygen/min × mg protein).

Mentions: Liver mitochondria from S rats displayed a significant decrease in basal and fatty acid-induced proton leak compared to L rats (Figure 4). In fact, at the same membrane potential (180 mV for basal leak) the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 37.5 ± 2.1; safflower-linseed = 10.5 ± 0.9 ngatoms oxygen/min × mg protein). Similarly, at the same membrane potential of 150 mV for a fatty acid-induced proton leak, the oxygen used to balance the proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 41.9 ± 2.2; safflower-linseed = 22.2 ± 1.1 ngatoms oxygen/min × mg protein).


Fat Quality Influences the Obesogenic Effect of High Fat Diets.

Crescenzo R, Bianco F, Mazzoli A, Giacco A, Cancelliere R, di Fabio G, Zarrelli A, Liverini G, Iossa S - Nutrients (2015)

Basal (A) and fatty acid-induced (B) proton leak in rats fed lard or safflower-linseed high fat diet. Values are reported as means with their standard errors. n = 8 different rats. Non-linear regression analysis show that curves for rats fed lard were significantly different than those of rats fed safflower-linseed (p < 0.05). In addition, at the same membrane potential (180 mV for basal leak) the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 37.5 ± 2.1; safflower-linseed = 10.5 ± 0.9 ngatoms oxygen/min × mg protein). Similarly, at the same membrane potential of 150 mV for fatty acid-induced proton leak, the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 41.9 ± 2.2; safflower-linseed = 22.2 ± 1.1 ngatoms oxygen/min × mg protein).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663608&req=5

nutrients-07-05480-f004: Basal (A) and fatty acid-induced (B) proton leak in rats fed lard or safflower-linseed high fat diet. Values are reported as means with their standard errors. n = 8 different rats. Non-linear regression analysis show that curves for rats fed lard were significantly different than those of rats fed safflower-linseed (p < 0.05). In addition, at the same membrane potential (180 mV for basal leak) the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 37.5 ± 2.1; safflower-linseed = 10.5 ± 0.9 ngatoms oxygen/min × mg protein). Similarly, at the same membrane potential of 150 mV for fatty acid-induced proton leak, the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 41.9 ± 2.2; safflower-linseed = 22.2 ± 1.1 ngatoms oxygen/min × mg protein).
Mentions: Liver mitochondria from S rats displayed a significant decrease in basal and fatty acid-induced proton leak compared to L rats (Figure 4). In fact, at the same membrane potential (180 mV for basal leak) the oxygen used to balance proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 37.5 ± 2.1; safflower-linseed = 10.5 ± 0.9 ngatoms oxygen/min × mg protein). Similarly, at the same membrane potential of 150 mV for a fatty acid-induced proton leak, the oxygen used to balance the proton leak is significantly (p < 0.05) higher in lard compared to safflower-linseed (lard = 41.9 ± 2.2; safflower-linseed = 22.2 ± 1.1 ngatoms oxygen/min × mg protein).

Bottom Line: Hepatic functionality, plasma parameters, and oxidative status were also measured.The results show that feeding on safflower/linseed oil diet attenuates the obesogenic effect of high fat diets and ameliorates the blood lipid profile.Conversely, hepatic steatosis and mitochondrial oxidative stress appear to be negatively affected by a diet rich in unsaturated fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Federico II University, Via Cinthia, 80138 Naples, Italy. rcrescen@unina.it.

ABSTRACT
High fat and/or carbohydrate intake are associated with an elevated risk for obesity and chronic diseases such as diabetes and cardiovascular diseases. The harmful effects of a high fat diet could be different, depending on dietary fat quality. In fact, high fat diets rich in unsaturated fatty acids are considered less deleterious for human health than those rich in saturated fat. In our previous studies, we have shown that rats fed a high fat diet developed obesity and exhibited a decrease in oxidative capacity and an increase in oxidative stress in liver mitochondria. To investigate whether polyunsaturated fats could attenuate the above deleterious effects of high fat diets, energy balance and body composition were assessed after two weeks in rats fed isocaloric amounts of a high-fat diet (58.2% by energy) rich either in lard or safflower/linseed oil. Hepatic functionality, plasma parameters, and oxidative status were also measured. The results show that feeding on safflower/linseed oil diet attenuates the obesogenic effect of high fat diets and ameliorates the blood lipid profile. Conversely, hepatic steatosis and mitochondrial oxidative stress appear to be negatively affected by a diet rich in unsaturated fatty acids.

No MeSH data available.


Related in: MedlinePlus