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Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus

The antioxidant properties of AQFE. Radical scavenging activity was determined by using different concentrations of AQFE (25–500 μg/mL) or vitamin C and trolox through DPPH (A) and ABTS+ (B) assays. The effect of AQFE was confirmed by measurement of reducing capacity (C). Effect of AQFE on intracellular ROS production in AGE-BSA treated human dermal fibroblast cells (D). Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group, #p < 0.05 compared with the AGE-BSA treated group, ##p < 0.01 compared with the AGE-BSA treated group (n = 5). The results were confirmed by eight independent experiments.
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nutrients-07-05478-f004: The antioxidant properties of AQFE. Radical scavenging activity was determined by using different concentrations of AQFE (25–500 μg/mL) or vitamin C and trolox through DPPH (A) and ABTS+ (B) assays. The effect of AQFE was confirmed by measurement of reducing capacity (C). Effect of AQFE on intracellular ROS production in AGE-BSA treated human dermal fibroblast cells (D). Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group, #p < 0.05 compared with the AGE-BSA treated group, ##p < 0.01 compared with the AGE-BSA treated group (n = 5). The results were confirmed by eight independent experiments.

Mentions: In the present work, several assays were employed to evaluate the antioxidant activity of AQFE (Figure 4). Radical-scavenging activity was assessed by the DPPH assay using vitamin C (50 μg/mL) and trolox (50 μg/mL) as comparative controls. DPPH scavenging activity of the various concentrations of AQFE (25–500 μg/mL) ranged from 22.10% ± 0.76% to 97.86% ± 0.00%, with an IC50 of 112.04 ± 0.40 μg/mL (Figure 4A). The results confirmed free radical-scavenging activity by AQFE in a dose-dependent manner. The ABTS+ scavenging capacity assay is another indicator of the antioxidant activity. AQFE at 500 μg/mL showed similar ABTS+ scavenging capacity as vitamin C (50 μg/mL) and trolox (50 μg/mL) (Figure 4B). The IC50 of AQFE was 160.79 ± 4.63 μg/mL. The results clearly demonstrated antioxidant capability of AQFE. To measure the reducing activity of AQFE, different concentrations of AQFE (25–500 μg/mL) or trolox (50 μg/mL) were used. AQFE at 500 μg/mL exhibited similar reducing capacity as trolox (Figure 4C), indicating its reducing power. The IC50 value of AQFE was compared to some pure antioxidant chemicals used commercially, including ascorbic acid and trolox. As shown in Figure 4, although the antioxidant capacity of AQFE was weaker than those of the pure antioxidants, it was still relatively strong compared to many edible vegetables and components of Chinese medicine [33].


Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

The antioxidant properties of AQFE. Radical scavenging activity was determined by using different concentrations of AQFE (25–500 μg/mL) or vitamin C and trolox through DPPH (A) and ABTS+ (B) assays. The effect of AQFE was confirmed by measurement of reducing capacity (C). Effect of AQFE on intracellular ROS production in AGE-BSA treated human dermal fibroblast cells (D). Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group, #p < 0.05 compared with the AGE-BSA treated group, ##p < 0.01 compared with the AGE-BSA treated group (n = 5). The results were confirmed by eight independent experiments.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4663606&req=5

nutrients-07-05478-f004: The antioxidant properties of AQFE. Radical scavenging activity was determined by using different concentrations of AQFE (25–500 μg/mL) or vitamin C and trolox through DPPH (A) and ABTS+ (B) assays. The effect of AQFE was confirmed by measurement of reducing capacity (C). Effect of AQFE on intracellular ROS production in AGE-BSA treated human dermal fibroblast cells (D). Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group, #p < 0.05 compared with the AGE-BSA treated group, ##p < 0.01 compared with the AGE-BSA treated group (n = 5). The results were confirmed by eight independent experiments.
Mentions: In the present work, several assays were employed to evaluate the antioxidant activity of AQFE (Figure 4). Radical-scavenging activity was assessed by the DPPH assay using vitamin C (50 μg/mL) and trolox (50 μg/mL) as comparative controls. DPPH scavenging activity of the various concentrations of AQFE (25–500 μg/mL) ranged from 22.10% ± 0.76% to 97.86% ± 0.00%, with an IC50 of 112.04 ± 0.40 μg/mL (Figure 4A). The results confirmed free radical-scavenging activity by AQFE in a dose-dependent manner. The ABTS+ scavenging capacity assay is another indicator of the antioxidant activity. AQFE at 500 μg/mL showed similar ABTS+ scavenging capacity as vitamin C (50 μg/mL) and trolox (50 μg/mL) (Figure 4B). The IC50 of AQFE was 160.79 ± 4.63 μg/mL. The results clearly demonstrated antioxidant capability of AQFE. To measure the reducing activity of AQFE, different concentrations of AQFE (25–500 μg/mL) or trolox (50 μg/mL) were used. AQFE at 500 μg/mL exhibited similar reducing capacity as trolox (Figure 4C), indicating its reducing power. The IC50 value of AQFE was compared to some pure antioxidant chemicals used commercially, including ascorbic acid and trolox. As shown in Figure 4, although the antioxidant capacity of AQFE was weaker than those of the pure antioxidants, it was still relatively strong compared to many edible vegetables and components of Chinese medicine [33].

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus