Limits...
Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus

Activity of AQFE against methylglyoxal-induced glycation, revealed by the immunostaining of CML. The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. CML immunostaining was conducted. Immunostaining of CML on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Staining intensity on batches treated with active ingredients on CML immunostaining. The average staining intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group; ##p < 0.01 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4663606&req=5

nutrients-07-05478-f003: Activity of AQFE against methylglyoxal-induced glycation, revealed by the immunostaining of CML. The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. CML immunostaining was conducted. Immunostaining of CML on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Staining intensity on batches treated with active ingredients on CML immunostaining. The average staining intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group; ##p < 0.01 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.

Mentions: A decrease in the staining of CML (Figure 3A–F) was also observed in the glycated explants treated with AG and AQFE unlike the explants treated with MG. MG induced a clear increase in CML expression on Day 8. The AQFE-treated batch (not stimulated with MG) showed complete inhibition of physiological CML expression. The AQFE-treated batch induced by MG showed complete inhibition of CML expression induced by MG. AQFE showed a clear inhibition of CML expression in both the MG-treated and non-MG-treated batches. These findings were confirmed by the results obtained from the image analysis of CML, presented in Figure 3G.


Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

Activity of AQFE against methylglyoxal-induced glycation, revealed by the immunostaining of CML. The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. CML immunostaining was conducted. Immunostaining of CML on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Staining intensity on batches treated with active ingredients on CML immunostaining. The average staining intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group; ##p < 0.01 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663606&req=5

nutrients-07-05478-f003: Activity of AQFE against methylglyoxal-induced glycation, revealed by the immunostaining of CML. The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. CML immunostaining was conducted. Immunostaining of CML on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Staining intensity on batches treated with active ingredients on CML immunostaining. The average staining intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group; ##p < 0.01 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.
Mentions: A decrease in the staining of CML (Figure 3A–F) was also observed in the glycated explants treated with AG and AQFE unlike the explants treated with MG. MG induced a clear increase in CML expression on Day 8. The AQFE-treated batch (not stimulated with MG) showed complete inhibition of physiological CML expression. The AQFE-treated batch induced by MG showed complete inhibition of CML expression induced by MG. AQFE showed a clear inhibition of CML expression in both the MG-treated and non-MG-treated batches. These findings were confirmed by the results obtained from the image analysis of CML, presented in Figure 3G.

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus