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Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus

The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. Fibrillin-1 immunostaining was conducted. Immunostaining of fibrillin-1 on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Image analysis of the surface percentage occupied by fibrillin-1 under the dermal-epidermal junction, as a function of the product applied. The average fluorescence intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.
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nutrients-07-05478-f002: The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. Fibrillin-1 immunostaining was conducted. Immunostaining of fibrillin-1 on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Image analysis of the surface percentage occupied by fibrillin-1 under the dermal-epidermal junction, as a function of the product applied. The average fluorescence intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.

Mentions: When applied topically to glycated explants, aminoguanidine showed the same protective effect on fibrillin-1 as when added to the culture medium in the second step. The fibrillin-1 network was unchanged compared to the control batch and was protected from methylglyoxal-induced glycation (Figure 2). The degradation of fibrillin-1 through methylglyoxal-induced glycation was clearly highlighted, with a decrease of 26.7% in the surface percentage occupied by fibrillin-1 under the dermal-epidermal junction (DEJ), compared to the untreated control batch. The AQFE applied topically also protected fibrillin-1 from glycation and the fibrillin-1 staining was similar to the control batch without induced glycation (Figure 2A–F). These findings were confirmed by the results obtained from the image analysis of fibrillin-1, presented in Figure 2G.


Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. Fibrillin-1 immunostaining was conducted. Immunostaining of fibrillin-1 on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Image analysis of the surface percentage occupied by fibrillin-1 under the dermal-epidermal junction, as a function of the product applied. The average fluorescence intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663606&req=5

nutrients-07-05478-f002: The explants were kept alive in a BEM culture medium for eight days with the vehicle and 1% AQFE. Fibrillin-1 immunostaining was conducted. Immunostaining of fibrillin-1 on untreated batch (A); treated with AG (B); treated with AQFE (C); treated with MG (D); treated with MG + AG (E) and treated with MG + AQFE (F). (G) Image analysis of the surface percentage occupied by fibrillin-1 under the dermal-epidermal junction, as a function of the product applied. The average fluorescence intensity values were calculated using Image J software. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the MG-treated group (n = 3). The results were confirmed by eight independent experiments.
Mentions: When applied topically to glycated explants, aminoguanidine showed the same protective effect on fibrillin-1 as when added to the culture medium in the second step. The fibrillin-1 network was unchanged compared to the control batch and was protected from methylglyoxal-induced glycation (Figure 2). The degradation of fibrillin-1 through methylglyoxal-induced glycation was clearly highlighted, with a decrease of 26.7% in the surface percentage occupied by fibrillin-1 under the dermal-epidermal junction (DEJ), compared to the untreated control batch. The AQFE applied topically also protected fibrillin-1 from glycation and the fibrillin-1 staining was similar to the control batch without induced glycation (Figure 2A–F). These findings were confirmed by the results obtained from the image analysis of fibrillin-1, presented in Figure 2G.

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus