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Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus

The effects of AQFE on formation of fluorescent advanced glycation end products in BSA incubated with glucose. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the BSA/glucose treated group; ##p < 0.01 compared with the BSA/glucose treated group (n = 5). The results were confirmed by eight independent experiments.
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nutrients-07-05478-f001: The effects of AQFE on formation of fluorescent advanced glycation end products in BSA incubated with glucose. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the BSA/glucose treated group; ##p < 0.01 compared with the BSA/glucose treated group (n = 5). The results were confirmed by eight independent experiments.

Mentions: In the present study, the AGE inhibitory capacities of AQFE were evaluated for the first time. The BSA-glucose system employed is commonly used in non-enzymatic glycation studies. In the BSA-glucose system, AQFE exhibited significant AGE inhibitory ability in a dose-dependent manner. As shown in Figure 1, the formation of AGEs was monitored at Week 3 by measuring the fluorescence intensity of the BSA-glucose solutions. When BSA was incubated with glucose, a significant increase in fluorescence intensity was observed at Week 3 of the experiment. After AQFE was added to the reaction media containing BSA/glucose, the fluorescence intensity decreased significantly in a concentration-dependent manner throughout the study period. At Week 3 of incubation, the percentage inhibition of AGEs formation by AQFE (10–500 µg/mL) was 3.0% to 37.4%, respectively.


Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products.

Shin S, Son D, Kim M, Lee S, Roh KB, Ryu D, Lee J, Jung E, Park D - Nutrients (2015)

The effects of AQFE on formation of fluorescent advanced glycation end products in BSA incubated with glucose. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the BSA/glucose treated group; ##p < 0.01 compared with the BSA/glucose treated group (n = 5). The results were confirmed by eight independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663606&req=5

nutrients-07-05478-f001: The effects of AQFE on formation of fluorescent advanced glycation end products in BSA incubated with glucose. Data are mean ± standard deviation. §p < 0.01 compared with the vehicle-treated group; #p < 0.05 compared with the BSA/glucose treated group; ##p < 0.01 compared with the BSA/glucose treated group (n = 5). The results were confirmed by eight independent experiments.
Mentions: In the present study, the AGE inhibitory capacities of AQFE were evaluated for the first time. The BSA-glucose system employed is commonly used in non-enzymatic glycation studies. In the BSA-glucose system, AQFE exhibited significant AGE inhibitory ability in a dose-dependent manner. As shown in Figure 1, the formation of AGEs was monitored at Week 3 by measuring the fluorescence intensity of the BSA-glucose solutions. When BSA was incubated with glucose, a significant increase in fluorescence intensity was observed at Week 3 of the experiment. After AQFE was added to the reaction media containing BSA/glucose, the fluorescence intensity decreased significantly in a concentration-dependent manner throughout the study period. At Week 3 of incubation, the percentage inhibition of AGEs formation by AQFE (10–500 µg/mL) was 3.0% to 37.4%, respectively.

Bottom Line: We also found that AQFE inhibits glycation reaction between BSA and glucose.AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment.The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

View Article: PubMed Central - PubMed

Affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.

ABSTRACT
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.

No MeSH data available.


Related in: MedlinePlus