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Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro.

Spilmont M, Léotoing L, Davicco MJ, Lebecque P, Miot-Noirault E, Pilet P, Rios L, Wittrant Y, Coxam V - Nutrients (2015)

Bottom Line: The nutritional benefits of pomegranate have attracted great scientific interest.Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia.In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers.

View Article: PubMed Central - PubMed

Affiliation: Unité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, France. mel.spilmont@gmail.com.

ABSTRACT
The nutritional benefits of pomegranate have attracted great scientific interest. The pomegranate, including the pomegranate peel, has been used worldwide for many years as a fruit with medicinal activity, mostly antioxidant properties. Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia. In this study, with in vivo and ex vivo experiments, we investigated whether the consumption of pomegranate peel extract (PGPE) could limit the process of osteopenia. We demonstrated that in ovariectomized (OVX) C57BL/6J mice, PGPE consumption was able to significantly prevent the decrease in bone mineral density (-31.9%; p < 0.001 vs. OVX mice) and bone microarchitecture impairment. Moreover, the exposure of RAW264.7 cells to serum harvested from mice that had been given a PGPE-enriched diet elicited reduced osteoclast differentiation and bone resorption, as shown by the inhibition of the major osteoclast markers. In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers. PGPE may be effective in preventing the bone loss associated with ovariectomy in mice, and offers a promising alternative for the nutritional management of this disease.

No MeSH data available.


Related in: MedlinePlus

Effect of ovariectomy and pomegranate peel extract consumption for 30 days on bone parameters in mice. (A) Bone mineral density analysis of left femur by X-ray radiation micro-CT. Mice were sham-operated, SH or ovariectomized, OVX and fed standard diet AIN-93 (Control) or standard diet enriched with 2g/kg of pomegranate peel extract, PGPE representing a dose of 10 mg polyphenols/kg body weight/day (ellagic acid equivalent) for 30 days. Each group contained 10 mice. Results are presented as means ± standard error of the mean, SEM. # p < 0.005 OVX Control versus SH Control group. * p < 0.05 OVX PGPE versus OVX Control. (B) Representative microCT images of the distal left femur for each group: SH Control, OVX Control and OVX PGPE. Scale bars, 1 mm.
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nutrients-07-05465-f004: Effect of ovariectomy and pomegranate peel extract consumption for 30 days on bone parameters in mice. (A) Bone mineral density analysis of left femur by X-ray radiation micro-CT. Mice were sham-operated, SH or ovariectomized, OVX and fed standard diet AIN-93 (Control) or standard diet enriched with 2g/kg of pomegranate peel extract, PGPE representing a dose of 10 mg polyphenols/kg body weight/day (ellagic acid equivalent) for 30 days. Each group contained 10 mice. Results are presented as means ± standard error of the mean, SEM. # p < 0.005 OVX Control versus SH Control group. * p < 0.05 OVX PGPE versus OVX Control. (B) Representative microCT images of the distal left femur for each group: SH Control, OVX Control and OVX PGPE. Scale bars, 1 mm.

Mentions: Bone morphological parameters using micro-CT analysis were first assessed to evaluate the respective effects of ovariectomy and PGPE consumption on mice. We confirmed the acknowledged effect of oestrogen deficiency on femoral BMD by comparing the OVX Control animals and the SH Control group. Indeed, the OVX Control mice had a significant lower BMD (p < 0.001) than the SH Control mice (−35.6%) (Figure 4A). PGPE consumption was associated with the prevention of such a bone decrease in OVX animals (+31.9%; p < 0.001 compared to the OVX Control animals).


Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro.

Spilmont M, Léotoing L, Davicco MJ, Lebecque P, Miot-Noirault E, Pilet P, Rios L, Wittrant Y, Coxam V - Nutrients (2015)

Effect of ovariectomy and pomegranate peel extract consumption for 30 days on bone parameters in mice. (A) Bone mineral density analysis of left femur by X-ray radiation micro-CT. Mice were sham-operated, SH or ovariectomized, OVX and fed standard diet AIN-93 (Control) or standard diet enriched with 2g/kg of pomegranate peel extract, PGPE representing a dose of 10 mg polyphenols/kg body weight/day (ellagic acid equivalent) for 30 days. Each group contained 10 mice. Results are presented as means ± standard error of the mean, SEM. # p < 0.005 OVX Control versus SH Control group. * p < 0.05 OVX PGPE versus OVX Control. (B) Representative microCT images of the distal left femur for each group: SH Control, OVX Control and OVX PGPE. Scale bars, 1 mm.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4663593&req=5

nutrients-07-05465-f004: Effect of ovariectomy and pomegranate peel extract consumption for 30 days on bone parameters in mice. (A) Bone mineral density analysis of left femur by X-ray radiation micro-CT. Mice were sham-operated, SH or ovariectomized, OVX and fed standard diet AIN-93 (Control) or standard diet enriched with 2g/kg of pomegranate peel extract, PGPE representing a dose of 10 mg polyphenols/kg body weight/day (ellagic acid equivalent) for 30 days. Each group contained 10 mice. Results are presented as means ± standard error of the mean, SEM. # p < 0.005 OVX Control versus SH Control group. * p < 0.05 OVX PGPE versus OVX Control. (B) Representative microCT images of the distal left femur for each group: SH Control, OVX Control and OVX PGPE. Scale bars, 1 mm.
Mentions: Bone morphological parameters using micro-CT analysis were first assessed to evaluate the respective effects of ovariectomy and PGPE consumption on mice. We confirmed the acknowledged effect of oestrogen deficiency on femoral BMD by comparing the OVX Control animals and the SH Control group. Indeed, the OVX Control mice had a significant lower BMD (p < 0.001) than the SH Control mice (−35.6%) (Figure 4A). PGPE consumption was associated with the prevention of such a bone decrease in OVX animals (+31.9%; p < 0.001 compared to the OVX Control animals).

Bottom Line: The nutritional benefits of pomegranate have attracted great scientific interest.Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia.In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers.

View Article: PubMed Central - PubMed

Affiliation: Unité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, France. mel.spilmont@gmail.com.

ABSTRACT
The nutritional benefits of pomegranate have attracted great scientific interest. The pomegranate, including the pomegranate peel, has been used worldwide for many years as a fruit with medicinal activity, mostly antioxidant properties. Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia. In this study, with in vivo and ex vivo experiments, we investigated whether the consumption of pomegranate peel extract (PGPE) could limit the process of osteopenia. We demonstrated that in ovariectomized (OVX) C57BL/6J mice, PGPE consumption was able to significantly prevent the decrease in bone mineral density (-31.9%; p < 0.001 vs. OVX mice) and bone microarchitecture impairment. Moreover, the exposure of RAW264.7 cells to serum harvested from mice that had been given a PGPE-enriched diet elicited reduced osteoclast differentiation and bone resorption, as shown by the inhibition of the major osteoclast markers. In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers. PGPE may be effective in preventing the bone loss associated with ovariectomy in mice, and offers a promising alternative for the nutritional management of this disease.

No MeSH data available.


Related in: MedlinePlus