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Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.

Boets E, Deroover L, Houben E, Vermeulen K, Gomand SV, Delcour JA, Verbeke K - Nutrients (2015)

Bottom Line: Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation.In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate.Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

View Article: PubMed Central - PubMed

Affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. eef.boets@med.kuleuven.be.

ABSTRACT
Short chain fatty acids (SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [1-(13)C]acetate, [1-(13)C]propionate and [1-(13)C]butyrate (12, 1.2 and 0.6 μmol·kg(-1)·min(-1), respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 ± 4.8, 0.27 ± 0.09, and 0.28 ± 0.12 μmol·kg(-1)·min(-1), respectively. Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

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Typical example that shows the 13C enrichment (APE) of butyrate in plasma over time and the whole-body rate of appearance (Ra) of butyrate over time. n = 1.
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nutrients-07-05440-f002: Typical example that shows the 13C enrichment (APE) of butyrate in plasma over time and the whole-body rate of appearance (Ra) of butyrate over time. n = 1.

Mentions: The Ra of SCFA increased significantly during fermentation of inulin (Table 3). A representative example of the 13C enrichment of butyrate and whole body Ra of butyrate in one subject is shown in Figure 2. A positive correlation was observed between whole-body Ra of propionate and butyrate (r = 0.657, p = 0.020). No correlations were observed between butyrate and acetate whole-body Ra and between propionate and acetate whole-body Ra.


Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.

Boets E, Deroover L, Houben E, Vermeulen K, Gomand SV, Delcour JA, Verbeke K - Nutrients (2015)

Typical example that shows the 13C enrichment (APE) of butyrate in plasma over time and the whole-body rate of appearance (Ra) of butyrate over time. n = 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663568&req=5

nutrients-07-05440-f002: Typical example that shows the 13C enrichment (APE) of butyrate in plasma over time and the whole-body rate of appearance (Ra) of butyrate over time. n = 1.
Mentions: The Ra of SCFA increased significantly during fermentation of inulin (Table 3). A representative example of the 13C enrichment of butyrate and whole body Ra of butyrate in one subject is shown in Figure 2. A positive correlation was observed between whole-body Ra of propionate and butyrate (r = 0.657, p = 0.020). No correlations were observed between butyrate and acetate whole-body Ra and between propionate and acetate whole-body Ra.

Bottom Line: Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation.In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate.Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

View Article: PubMed Central - PubMed

Affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. eef.boets@med.kuleuven.be.

ABSTRACT
Short chain fatty acids (SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [1-(13)C]acetate, [1-(13)C]propionate and [1-(13)C]butyrate (12, 1.2 and 0.6 μmol·kg(-1)·min(-1), respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 ± 4.8, 0.27 ± 0.09, and 0.28 ± 0.12 μmol·kg(-1)·min(-1), respectively. Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

Show MeSH