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Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.

Boets E, Deroover L, Houben E, Vermeulen K, Gomand SV, Delcour JA, Verbeke K - Nutrients (2015)

Bottom Line: Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation.In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate.Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

View Article: PubMed Central - PubMed

Affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. eef.boets@med.kuleuven.be.

ABSTRACT
Short chain fatty acids (SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [1-(13)C]acetate, [1-(13)C]propionate and [1-(13)C]butyrate (12, 1.2 and 0.6 μmol·kg(-1)·min(-1), respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 ± 4.8, 0.27 ± 0.09, and 0.28 ± 0.12 μmol·kg(-1)·min(-1), respectively. Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

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Correlation between acetate, propionate and butyrate endogenous rate of appearance (Ra) and body mass index (BMI). n = 12.
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nutrients-07-05440-f001: Correlation between acetate, propionate and butyrate endogenous rate of appearance (Ra) and body mass index (BMI). n = 12.

Mentions: Endogenous Ra of propionate showed a significantly positive correlation with acetate (r = 0.652, p = 0.022). No correlation was observed between the endogenous Ra of butyrate and acetate (r = 0.478, p = 0.116) and between Ra of butyrate and propionate (r = 0.551, p = 0.063). Propionate (r = −0.644, p = 0.024) and butyrate (r = −0.729, p = 0.007) endogenous Ra were significantly negatively correlated with body mass index (BMI) (Figure 1).


Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.

Boets E, Deroover L, Houben E, Vermeulen K, Gomand SV, Delcour JA, Verbeke K - Nutrients (2015)

Correlation between acetate, propionate and butyrate endogenous rate of appearance (Ra) and body mass index (BMI). n = 12.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663568&req=5

nutrients-07-05440-f001: Correlation between acetate, propionate and butyrate endogenous rate of appearance (Ra) and body mass index (BMI). n = 12.
Mentions: Endogenous Ra of propionate showed a significantly positive correlation with acetate (r = 0.652, p = 0.022). No correlation was observed between the endogenous Ra of butyrate and acetate (r = 0.478, p = 0.116) and between Ra of butyrate and propionate (r = 0.551, p = 0.063). Propionate (r = −0.644, p = 0.024) and butyrate (r = −0.729, p = 0.007) endogenous Ra were significantly negatively correlated with body mass index (BMI) (Figure 1).

Bottom Line: Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation.In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate.Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

View Article: PubMed Central - PubMed

Affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. eef.boets@med.kuleuven.be.

ABSTRACT
Short chain fatty acids (SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [1-(13)C]acetate, [1-(13)C]propionate and [1-(13)C]butyrate (12, 1.2 and 0.6 μmol·kg(-1)·min(-1), respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 ± 4.8, 0.27 ± 0.09, and 0.28 ± 0.12 μmol·kg(-1)·min(-1), respectively. Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.

Show MeSH