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Interactions between Carotenoids from Marine Bacteria and Other Micronutrients: Impact on Stability and Antioxidant Activity.

Sy C, Dangles O, Borel P, Caris-Veyrat C - Mar Drugs (2015)

Bottom Line: For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually.Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron.Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR408 SQPOV, F-84000 Avignon, France. sy_charlotte@hotmail.com.

ABSTRACT
Recently isolated spore-forming pigmented marine bacteria Bacillus indicus HU36 are sources of oxygenated carotenoids with original structures (about fifteen distinct yellow and orange pigments with acylated d-glucosyl groups). In this study, we evaluated the stability (sensitivity to iron-induced autoxidation) and antioxidant activity (inhibition of iron-induced lipid peroxidation) of combinations of bacterial HU36 carotenoids with the bacterial vitamin menaquinone MQ-7 and with phenolic antioxidants (vitamin E, chlorogenic acid, rutin). Unexpectedly, MQ-7 strongly improves the ability of HU36 carotenoids to inhibit Fe(II)-induced lipid peroxidation, although MQ-7 was not consumed in the medium. We propose that their interaction modifies the carotenoid antioxidant mechanism(s), possibly by allowing carotenoids to scavenge the initiating radicals. For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually. HU36 carotenoids and phenolic antioxidants displayed synergistic activities in the inhibition of linoleic acid peroxidation induced by heme iron, but not by free iron. Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron. Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail.

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Iron-induced autoxidation of β-carotene (10 μmol·L−1) without and with MQ-7 (10 and 100 μmol·L−1). Kinetic monitoring at pH 4 after addition of (A) 50 μmol·L−1 FeII; and (B) 100 μmol·L−1 FeIII and (C) 0.5 μmol·L−1 MbFeIII as oxidation initiators. Percentages represent the final concentration of β-carotene remaining at the end of the experiment relative to t = 0 min.
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marinedrugs-13-07020-f001: Iron-induced autoxidation of β-carotene (10 μmol·L−1) without and with MQ-7 (10 and 100 μmol·L−1). Kinetic monitoring at pH 4 after addition of (A) 50 μmol·L−1 FeII; and (B) 100 μmol·L−1 FeIII and (C) 0.5 μmol·L−1 MbFeIII as oxidation initiators. Percentages represent the final concentration of β-carotene remaining at the end of the experiment relative to t = 0 min.

Mentions: Influence of MQ-7 on the stability of β-carotene in the presence of iron. The stability of β-carotene was compared in the presence and absence of purified MQ-7 at two concentrations (10 and 100 μmol·L−1). The kinetic profiles of the curves were similar, but MQ-7 lowered β-carotene degradation, especially in the presence of heme iron (Figure 1A–C). Stability of β-carotene toward heme-induced autoxidation was increased two-fold with 1 equiv. MQ-7 and three- to four-fold with 10 equiv. MQ-7.


Interactions between Carotenoids from Marine Bacteria and Other Micronutrients: Impact on Stability and Antioxidant Activity.

Sy C, Dangles O, Borel P, Caris-Veyrat C - Mar Drugs (2015)

Iron-induced autoxidation of β-carotene (10 μmol·L−1) without and with MQ-7 (10 and 100 μmol·L−1). Kinetic monitoring at pH 4 after addition of (A) 50 μmol·L−1 FeII; and (B) 100 μmol·L−1 FeIII and (C) 0.5 μmol·L−1 MbFeIII as oxidation initiators. Percentages represent the final concentration of β-carotene remaining at the end of the experiment relative to t = 0 min.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663564&req=5

marinedrugs-13-07020-f001: Iron-induced autoxidation of β-carotene (10 μmol·L−1) without and with MQ-7 (10 and 100 μmol·L−1). Kinetic monitoring at pH 4 after addition of (A) 50 μmol·L−1 FeII; and (B) 100 μmol·L−1 FeIII and (C) 0.5 μmol·L−1 MbFeIII as oxidation initiators. Percentages represent the final concentration of β-carotene remaining at the end of the experiment relative to t = 0 min.
Mentions: Influence of MQ-7 on the stability of β-carotene in the presence of iron. The stability of β-carotene was compared in the presence and absence of purified MQ-7 at two concentrations (10 and 100 μmol·L−1). The kinetic profiles of the curves were similar, but MQ-7 lowered β-carotene degradation, especially in the presence of heme iron (Figure 1A–C). Stability of β-carotene toward heme-induced autoxidation was increased two-fold with 1 equiv. MQ-7 and three- to four-fold with 10 equiv. MQ-7.

Bottom Line: For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually.Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron.Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR408 SQPOV, F-84000 Avignon, France. sy_charlotte@hotmail.com.

ABSTRACT
Recently isolated spore-forming pigmented marine bacteria Bacillus indicus HU36 are sources of oxygenated carotenoids with original structures (about fifteen distinct yellow and orange pigments with acylated d-glucosyl groups). In this study, we evaluated the stability (sensitivity to iron-induced autoxidation) and antioxidant activity (inhibition of iron-induced lipid peroxidation) of combinations of bacterial HU36 carotenoids with the bacterial vitamin menaquinone MQ-7 and with phenolic antioxidants (vitamin E, chlorogenic acid, rutin). Unexpectedly, MQ-7 strongly improves the ability of HU36 carotenoids to inhibit Fe(II)-induced lipid peroxidation, although MQ-7 was not consumed in the medium. We propose that their interaction modifies the carotenoid antioxidant mechanism(s), possibly by allowing carotenoids to scavenge the initiating radicals. For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually. HU36 carotenoids and phenolic antioxidants displayed synergistic activities in the inhibition of linoleic acid peroxidation induced by heme iron, but not by free iron. Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron. Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail.

Show MeSH