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Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice.

Park EY, Choi H, Yoon JY, Lee IY, Seo Y, Moon HS, Hwang JH, Jun HS - Mar Drugs (2015)

Bottom Line: Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue.Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism.The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. parkey@mokpo.ac.kr.

ABSTRACT
Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.

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Effect of G-CA on serum biochemical parameters. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. After 10 weeks, blood samples were collected and measured biochemical parameters (n = 5–6): (A) alanine aminotransferase (ALT); (B) aspartate aminotransferase (AST); (C) triglycerides; (D) cholesterol; (E) high density lipoprotein (HDL)-cholesterol; (F) low density lipoprotein (LDL)-cholesterol; and (G) atherogenic index. NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. * p < 0.05, ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.
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marinedrugs-13-06866-f004: Effect of G-CA on serum biochemical parameters. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. After 10 weeks, blood samples were collected and measured biochemical parameters (n = 5–6): (A) alanine aminotransferase (ALT); (B) aspartate aminotransferase (AST); (C) triglycerides; (D) cholesterol; (E) high density lipoprotein (HDL)-cholesterol; (F) low density lipoprotein (LDL)-cholesterol; and (G) atherogenic index. NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. * p < 0.05, ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.

Mentions: Excessive fat accumulation in the liver causes hepatocellular injury [24]. To evaluate whether G-CA treatment improves liver injury, serum ALT and AST levels were measured. Serum ALT and AST levels in the HFD-fed mice were significantly increased compared with NC mice, (444% and 175%, respectively; Figure 4A,B). Both ALT and AST levels were decreased in G-CA-HFD mice, but only serum ALT levels were statistically significant (Figure 4A,B; about 40.2% decrease). Next, to determine whether treatment with G-CA extract improves lipid metabolism, we measured the serum TG and cholesterol levels. The serum TG, total cholesterol, HDL-cholesterol and LDL-cholesterol levels were significantly higher in HFD mice compared with NC mice, whereas these serum lipid levels were significantly lower in G-CA-HFD mice compared with PBS-HFD mice (Figure 4C–F). Analysis of the atherogenic index showed that dyslipidemia caused by obesity was also improved by G-CA treatment (Figure 4G). These results suggest that G-CA extract treatment ameliorated liver injury and reduced lipid profiles.


Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice.

Park EY, Choi H, Yoon JY, Lee IY, Seo Y, Moon HS, Hwang JH, Jun HS - Mar Drugs (2015)

Effect of G-CA on serum biochemical parameters. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. After 10 weeks, blood samples were collected and measured biochemical parameters (n = 5–6): (A) alanine aminotransferase (ALT); (B) aspartate aminotransferase (AST); (C) triglycerides; (D) cholesterol; (E) high density lipoprotein (HDL)-cholesterol; (F) low density lipoprotein (LDL)-cholesterol; and (G) atherogenic index. NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. * p < 0.05, ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663557&req=5

marinedrugs-13-06866-f004: Effect of G-CA on serum biochemical parameters. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. After 10 weeks, blood samples were collected and measured biochemical parameters (n = 5–6): (A) alanine aminotransferase (ALT); (B) aspartate aminotransferase (AST); (C) triglycerides; (D) cholesterol; (E) high density lipoprotein (HDL)-cholesterol; (F) low density lipoprotein (LDL)-cholesterol; and (G) atherogenic index. NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. * p < 0.05, ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.
Mentions: Excessive fat accumulation in the liver causes hepatocellular injury [24]. To evaluate whether G-CA treatment improves liver injury, serum ALT and AST levels were measured. Serum ALT and AST levels in the HFD-fed mice were significantly increased compared with NC mice, (444% and 175%, respectively; Figure 4A,B). Both ALT and AST levels were decreased in G-CA-HFD mice, but only serum ALT levels were statistically significant (Figure 4A,B; about 40.2% decrease). Next, to determine whether treatment with G-CA extract improves lipid metabolism, we measured the serum TG and cholesterol levels. The serum TG, total cholesterol, HDL-cholesterol and LDL-cholesterol levels were significantly higher in HFD mice compared with NC mice, whereas these serum lipid levels were significantly lower in G-CA-HFD mice compared with PBS-HFD mice (Figure 4C–F). Analysis of the atherogenic index showed that dyslipidemia caused by obesity was also improved by G-CA treatment (Figure 4G). These results suggest that G-CA extract treatment ameliorated liver injury and reduced lipid profiles.

Bottom Line: Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue.Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism.The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. parkey@mokpo.ac.kr.

ABSTRACT
Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.

Show MeSH
Related in: MedlinePlus