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Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice.

Park EY, Choi H, Yoon JY, Lee IY, Seo Y, Moon HS, Hwang JH, Jun HS - Mar Drugs (2015)

Bottom Line: Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue.Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism.The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. parkey@mokpo.ac.kr.

ABSTRACT
Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.

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Effect of G-CA on hepatic lipid accumulation. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily for 10 weeks. (A) Hepatic triglyceride (TG) content was measured in liver tissue (n = 5–6); (B) Oil Red O staining was performed on frozen liver sections; (C) The right panels show typical liver 1H MR spectra from the selected region denoted as the white square in MRI in the left in PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p <0.01 vs. NC group; ##p <0.01 vs. PBS-HFD group.
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marinedrugs-13-06866-f003: Effect of G-CA on hepatic lipid accumulation. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily for 10 weeks. (A) Hepatic triglyceride (TG) content was measured in liver tissue (n = 5–6); (B) Oil Red O staining was performed on frozen liver sections; (C) The right panels show typical liver 1H MR spectra from the selected region denoted as the white square in MRI in the left in PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p <0.01 vs. NC group; ##p <0.01 vs. PBS-HFD group.

Mentions: To examine whether G-CA treatment can ameliorate hepatic steatosis, we measured TG levels in HFD-fed mice. The HFD feeding increased hepatic TG levels by about 550% (Figure 3A). The HFD-induced increases in hepatic TG levels were significantly decreased by G-CA treatment (Figure 3A; about 26% decrease). Measurement of hepatic lipid contents in frozen section showed that lipid droplet accumulation reduced in G-CA-HFD mice compared with that in PBS-HFD mice (Figure 3B). MRS measurements were also performed to quantify hepatic lipid content; the results showed that the fat contents of the liver were reduced by about 22% in G-CA-HFD mice compared with PBS-HFD mice (Figure 3C).


Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice.

Park EY, Choi H, Yoon JY, Lee IY, Seo Y, Moon HS, Hwang JH, Jun HS - Mar Drugs (2015)

Effect of G-CA on hepatic lipid accumulation. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily for 10 weeks. (A) Hepatic triglyceride (TG) content was measured in liver tissue (n = 5–6); (B) Oil Red O staining was performed on frozen liver sections; (C) The right panels show typical liver 1H MR spectra from the selected region denoted as the white square in MRI in the left in PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p <0.01 vs. NC group; ##p <0.01 vs. PBS-HFD group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4663557&req=5

marinedrugs-13-06866-f003: Effect of G-CA on hepatic lipid accumulation. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily for 10 weeks. (A) Hepatic triglyceride (TG) content was measured in liver tissue (n = 5–6); (B) Oil Red O staining was performed on frozen liver sections; (C) The right panels show typical liver 1H MR spectra from the selected region denoted as the white square in MRI in the left in PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p <0.01 vs. NC group; ##p <0.01 vs. PBS-HFD group.
Mentions: To examine whether G-CA treatment can ameliorate hepatic steatosis, we measured TG levels in HFD-fed mice. The HFD feeding increased hepatic TG levels by about 550% (Figure 3A). The HFD-induced increases in hepatic TG levels were significantly decreased by G-CA treatment (Figure 3A; about 26% decrease). Measurement of hepatic lipid contents in frozen section showed that lipid droplet accumulation reduced in G-CA-HFD mice compared with that in PBS-HFD mice (Figure 3B). MRS measurements were also performed to quantify hepatic lipid content; the results showed that the fat contents of the liver were reduced by about 22% in G-CA-HFD mice compared with PBS-HFD mice (Figure 3C).

Bottom Line: Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue.Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism.The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. parkey@mokpo.ac.kr.

ABSTRACT
Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.

Show MeSH
Related in: MedlinePlus