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Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice.

Park EY, Choi H, Yoon JY, Lee IY, Seo Y, Moon HS, Hwang JH, Jun HS - Mar Drugs (2015)

Bottom Line: Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue.Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism.The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. parkey@mokpo.ac.kr.

ABSTRACT
Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.

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Effect of G-CA on liver volume and liver tissue weight. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. (A) After 10 weeks of G-CA treatment, figure shows typical MRIs of liver of PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). The left panels show T1-weighted MRI including the liver. The sections in orange-brown represent liver in the segmented MRIs. (B) The graph depicts the difference in the total liver volumes in PBS-HFD and G-CA-HFD groups. (C) After 10 weeks of G-CA treatment, liver tissue was collected and weighed (NC: n = 5, PBS-HFD: n = 9, G-CA-HFD: n = 7). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.
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marinedrugs-13-06866-f002: Effect of G-CA on liver volume and liver tissue weight. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. (A) After 10 weeks of G-CA treatment, figure shows typical MRIs of liver of PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). The left panels show T1-weighted MRI including the liver. The sections in orange-brown represent liver in the segmented MRIs. (B) The graph depicts the difference in the total liver volumes in PBS-HFD and G-CA-HFD groups. (C) After 10 weeks of G-CA treatment, liver tissue was collected and weighed (NC: n = 5, PBS-HFD: n = 9, G-CA-HFD: n = 7). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.

Mentions: Numerous studies had reported increased liver weight in HFD-induced NAFLD as a consequence of hepatic lipid accumulation [22,23]. To examine whether treatment with G-CA extract affects liver content in HFD-induced NAFLD, we measured the volume of liver using MRI (Figure 2A). As shown in Figure 2B, liver volume significantly decreased in the G-CA-HFD group compared with that of the PBS-HFD group (35.56% decrease). When we measured liver weight after sacrifice, the increase in liver weight observed in the HFD groups was significantly attenuated by G-CA treatment (Figure 2C).


Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice.

Park EY, Choi H, Yoon JY, Lee IY, Seo Y, Moon HS, Hwang JH, Jun HS - Mar Drugs (2015)

Effect of G-CA on liver volume and liver tissue weight. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. (A) After 10 weeks of G-CA treatment, figure shows typical MRIs of liver of PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). The left panels show T1-weighted MRI including the liver. The sections in orange-brown represent liver in the segmented MRIs. (B) The graph depicts the difference in the total liver volumes in PBS-HFD and G-CA-HFD groups. (C) After 10 weeks of G-CA treatment, liver tissue was collected and weighed (NC: n = 5, PBS-HFD: n = 9, G-CA-HFD: n = 7). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663557&req=5

marinedrugs-13-06866-f002: Effect of G-CA on liver volume and liver tissue weight. Six weeks after beginning a high fat diet, C57BL6 mice were orally administered G-CA (300 mg/kg body weight) or PBS daily. (A) After 10 weeks of G-CA treatment, figure shows typical MRIs of liver of PBS-HFD (n = 5) and G-CA-HFD groups (n = 5). The left panels show T1-weighted MRI including the liver. The sections in orange-brown represent liver in the segmented MRIs. (B) The graph depicts the difference in the total liver volumes in PBS-HFD and G-CA-HFD groups. (C) After 10 weeks of G-CA treatment, liver tissue was collected and weighed (NC: n = 5, PBS-HFD: n = 9, G-CA-HFD: n = 7). NC: untreated, normal chow diet; PBS-HFD: PBS-treated, high fat diet (HFD); and G-CA-HFD: G-CA-treated, HFD. Values are mean ± SE. ** p < 0.01 vs. NC group; #p < 0.05, ##p < 0.01 vs. PBS-HFD group.
Mentions: Numerous studies had reported increased liver weight in HFD-induced NAFLD as a consequence of hepatic lipid accumulation [22,23]. To examine whether treatment with G-CA extract affects liver content in HFD-induced NAFLD, we measured the volume of liver using MRI (Figure 2A). As shown in Figure 2B, liver volume significantly decreased in the G-CA-HFD group compared with that of the PBS-HFD group (35.56% decrease). When we measured liver weight after sacrifice, the increase in liver weight observed in the HFD groups was significantly attenuated by G-CA treatment (Figure 2C).

Bottom Line: Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue.Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism.The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. parkey@mokpo.ac.kr.

ABSTRACT
Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.

Show MeSH
Related in: MedlinePlus