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Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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(a) Phase contrast microscopic images of gold nanoparticles induced gross cytomorphological changes and growth inhibition at different time (24 h and 48 h) intervals on the HeLa cells; (b) 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining shows apoptotic and necrotic cell death due to the cytotoxicity of biosynthesized gold nanoparticles (24 h and 48 h).
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marinedrugs-13-06818-f007: (a) Phase contrast microscopic images of gold nanoparticles induced gross cytomorphological changes and growth inhibition at different time (24 h and 48 h) intervals on the HeLa cells; (b) 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining shows apoptotic and necrotic cell death due to the cytotoxicity of biosynthesized gold nanoparticles (24 h and 48 h).

Mentions: The diverse morphological alteration was observed in gold nanoparticles-treated HeLa cells; however, no such effects were seen in untreated cells. It was shown that the morphological variations were observed such as the loss of membrane integrity, inhibition of cell growth, cytoplasmic condensation, and cell clumping, indicating that the gold nanoparticles treated HeLa cells undergone cell death, whereas the non-treated cell were active (Figure 7a).


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

(a) Phase contrast microscopic images of gold nanoparticles induced gross cytomorphological changes and growth inhibition at different time (24 h and 48 h) intervals on the HeLa cells; (b) 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining shows apoptotic and necrotic cell death due to the cytotoxicity of biosynthesized gold nanoparticles (24 h and 48 h).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f007: (a) Phase contrast microscopic images of gold nanoparticles induced gross cytomorphological changes and growth inhibition at different time (24 h and 48 h) intervals on the HeLa cells; (b) 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining shows apoptotic and necrotic cell death due to the cytotoxicity of biosynthesized gold nanoparticles (24 h and 48 h).
Mentions: The diverse morphological alteration was observed in gold nanoparticles-treated HeLa cells; however, no such effects were seen in untreated cells. It was shown that the morphological variations were observed such as the loss of membrane integrity, inhibition of cell growth, cytoplasmic condensation, and cell clumping, indicating that the gold nanoparticles treated HeLa cells undergone cell death, whereas the non-treated cell were active (Figure 7a).

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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