Limits...
Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

Show MeSH

Related in: MedlinePlus

MTT assay results confirming the in vitro cytotoxicity effect of gold nanoparticles against the HeLa cells for 24 h and 48 h respectively. Data is expressed as mean ± SD of three experiments. Percentage of cytotoxicity is expressed relative to untreated controls (* significant p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f006: MTT assay results confirming the in vitro cytotoxicity effect of gold nanoparticles against the HeLa cells for 24 h and 48 h respectively. Data is expressed as mean ± SD of three experiments. Percentage of cytotoxicity is expressed relative to untreated controls (* significant p < 0.05).

Mentions: Gold nanoparticles as novel agents for cancer therapy are gaining greater demand in medical applications. At the same time, there are only limited studies on the cytotoxic activity of biosynthesized gold nanoparticles against cancer cell lines. MTT assay was used to assess the effect of gold nanoparticles on the proliferation of HeLa cells. In the present study, we investigated that the induction of apoptosis could be the possible mechanism for the antiproliferative activity of biosynthesized gold nanoparticles. The dose-dependent cytotoxicity was observed in gold nanoparticle-treated HeLa cells. Fifty percent of cell death, determining the inhibitory concentration (IC50) value of biosynthesized gold nanoparticles against HeLa cells holds at 350 µg/mL in 24 h and 250 µg/mL in 48 h (Figure 6a). The experimental results clearly proved the excellent anticancer activity of gold nanoparticles against the HeLa cell line [31,32].


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

MTT assay results confirming the in vitro cytotoxicity effect of gold nanoparticles against the HeLa cells for 24 h and 48 h respectively. Data is expressed as mean ± SD of three experiments. Percentage of cytotoxicity is expressed relative to untreated controls (* significant p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f006: MTT assay results confirming the in vitro cytotoxicity effect of gold nanoparticles against the HeLa cells for 24 h and 48 h respectively. Data is expressed as mean ± SD of three experiments. Percentage of cytotoxicity is expressed relative to untreated controls (* significant p < 0.05).
Mentions: Gold nanoparticles as novel agents for cancer therapy are gaining greater demand in medical applications. At the same time, there are only limited studies on the cytotoxic activity of biosynthesized gold nanoparticles against cancer cell lines. MTT assay was used to assess the effect of gold nanoparticles on the proliferation of HeLa cells. In the present study, we investigated that the induction of apoptosis could be the possible mechanism for the antiproliferative activity of biosynthesized gold nanoparticles. The dose-dependent cytotoxicity was observed in gold nanoparticle-treated HeLa cells. Fifty percent of cell death, determining the inhibitory concentration (IC50) value of biosynthesized gold nanoparticles against HeLa cells holds at 350 µg/mL in 24 h and 250 µg/mL in 48 h (Figure 6a). The experimental results clearly proved the excellent anticancer activity of gold nanoparticles against the HeLa cell line [31,32].

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

Show MeSH
Related in: MedlinePlus