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Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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(a) Energy dispersive X-ray spectrum of biosynthesized gold nanoparticles; (b) High resolution transmission electron microscopy (HRTEM) images of biosynthesized gold nanoparticles (20 nm and 5 nm scale) and selected area electron diffraction (SAED) pattern.
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marinedrugs-13-06818-f005: (a) Energy dispersive X-ray spectrum of biosynthesized gold nanoparticles; (b) High resolution transmission electron microscopy (HRTEM) images of biosynthesized gold nanoparticles (20 nm and 5 nm scale) and selected area electron diffraction (SAED) pattern.

Mentions: Field emission scanning electron microscopy (FESEM) clearly shows the presence of the biosynthesized gold nanoparticles, which were confirmed to be gold by energy dispersive X-ray analysis (EDXA). EDXA (Figure 5a) spectrum represents the signal from Au+ ions together with Cl and O. Signals appear from Cl and O because of the X-ray emission from the biological macromolecules like proteins/enzymes bound to the nanoparticles or in the vicinity of the particles. The size and morphology of the biosynthesized gold nanoparticles was confirmed by high-resolution transmission electron microscopy (HRTEM) analysis. Figure 5b shows the mixture of gold nanospheres and gold nanoprisms (mostly truncated triangles). Most of the nanoparticles formed by this green method are well separated, and have a diameter in the range of 10–50 nm with an average particle size of 39 ± 2 nm. According to the face-centered cubic structure of biosynthesized gold nanoparticles, the spots in the selected area electron diffraction (SAED) pattern are indexed and reveal that the particles are single, and crystalline in nature. Previous studies report that HRTEM images for the biosynthesized gold nanoparticles are spherical in shape with an average size ranging from 10 to 100 nm [29,30].


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

(a) Energy dispersive X-ray spectrum of biosynthesized gold nanoparticles; (b) High resolution transmission electron microscopy (HRTEM) images of biosynthesized gold nanoparticles (20 nm and 5 nm scale) and selected area electron diffraction (SAED) pattern.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f005: (a) Energy dispersive X-ray spectrum of biosynthesized gold nanoparticles; (b) High resolution transmission electron microscopy (HRTEM) images of biosynthesized gold nanoparticles (20 nm and 5 nm scale) and selected area electron diffraction (SAED) pattern.
Mentions: Field emission scanning electron microscopy (FESEM) clearly shows the presence of the biosynthesized gold nanoparticles, which were confirmed to be gold by energy dispersive X-ray analysis (EDXA). EDXA (Figure 5a) spectrum represents the signal from Au+ ions together with Cl and O. Signals appear from Cl and O because of the X-ray emission from the biological macromolecules like proteins/enzymes bound to the nanoparticles or in the vicinity of the particles. The size and morphology of the biosynthesized gold nanoparticles was confirmed by high-resolution transmission electron microscopy (HRTEM) analysis. Figure 5b shows the mixture of gold nanospheres and gold nanoprisms (mostly truncated triangles). Most of the nanoparticles formed by this green method are well separated, and have a diameter in the range of 10–50 nm with an average particle size of 39 ± 2 nm. According to the face-centered cubic structure of biosynthesized gold nanoparticles, the spots in the selected area electron diffraction (SAED) pattern are indexed and reveal that the particles are single, and crystalline in nature. Previous studies report that HRTEM images for the biosynthesized gold nanoparticles are spherical in shape with an average size ranging from 10 to 100 nm [29,30].

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

Show MeSH
Related in: MedlinePlus