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Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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(a) UV-vis spectral analysis of biosynthesized gold nanoparticles; (b) Fourier transform infrared spectroscopy (FTIR) spectra of biosynthesized gold nanoparticles; (c) XRD pattern of biosynthesized gold nanoparticles.
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marinedrugs-13-06818-f004: (a) UV-vis spectral analysis of biosynthesized gold nanoparticles; (b) Fourier transform infrared spectroscopy (FTIR) spectra of biosynthesized gold nanoparticles; (c) XRD pattern of biosynthesized gold nanoparticles.

Mentions: The purified fucoidanase was added to an aqueous solution of gold chloride at 80 °C for 30 min, resulting in a color changing of solution that indicates the formation of gold nanoparticles observed by UV-vis spectroscopy. The biosynthesis of gold nanoparticles was evidenced through the appearance of an intense pinkish ruby red color because of the reduction of Au+ and formation of stable gold nanoparticles. The results indicated that the reaction solution showed an absorption peak at 531 nm attributed to the surface plasmon resonance band of the gold nanoparticles (Figure 4a). Various research groups have reported a change in the color from yellowish to pinkish ruby red after biotransformation during the biosynthetic process [19,23,24]. Although the common underlying mechanism involved in biosynthesis is the reduction of gold ions (Au+3) to form gold nanoparticles, it has been postulated that the enzymes secreted by microorganisms play an important role in the bioreduction of metal ions, leading to nanoparticle nucleation and growth [23]. Despite the enormous number of reports on microbially mediated gold nanoparticles synthesis, the mechanistic characteristics have not been established and need to be reported in depth [18,19,23].


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

(a) UV-vis spectral analysis of biosynthesized gold nanoparticles; (b) Fourier transform infrared spectroscopy (FTIR) spectra of biosynthesized gold nanoparticles; (c) XRD pattern of biosynthesized gold nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f004: (a) UV-vis spectral analysis of biosynthesized gold nanoparticles; (b) Fourier transform infrared spectroscopy (FTIR) spectra of biosynthesized gold nanoparticles; (c) XRD pattern of biosynthesized gold nanoparticles.
Mentions: The purified fucoidanase was added to an aqueous solution of gold chloride at 80 °C for 30 min, resulting in a color changing of solution that indicates the formation of gold nanoparticles observed by UV-vis spectroscopy. The biosynthesis of gold nanoparticles was evidenced through the appearance of an intense pinkish ruby red color because of the reduction of Au+ and formation of stable gold nanoparticles. The results indicated that the reaction solution showed an absorption peak at 531 nm attributed to the surface plasmon resonance band of the gold nanoparticles (Figure 4a). Various research groups have reported a change in the color from yellowish to pinkish ruby red after biotransformation during the biosynthetic process [19,23,24]. Although the common underlying mechanism involved in biosynthesis is the reduction of gold ions (Au+3) to form gold nanoparticles, it has been postulated that the enzymes secreted by microorganisms play an important role in the bioreduction of metal ions, leading to nanoparticle nucleation and growth [23]. Despite the enormous number of reports on microbially mediated gold nanoparticles synthesis, the mechanistic characteristics have not been established and need to be reported in depth [18,19,23].

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

Show MeSH